CD8 antibody
Principal name
CD8 antibody
Alternative names for CD8 antibody
CD8 alpha chain, CD8A, MAL, T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain
SwissProt ID
P01731 (Mouse), P01732 (Human), P07725 (Rat), P31783 (Bovin), P33706 (Canfa), P41688 (Felca), Q0R4I4 (Chick), Q6R2U9 (Pig)
Ncbi ID
NP_113726.1, NP_001074579, NP_001074579.1, NP_001759, NP_001139345.1, 9606, NP_776440, NP_001009843.1, NP_001009843, NP_001002935, NP_001001907.1, NP_776440.1, NP_001001907, 9031, 10090, NP_001139345, AAH25715, NP_741969, BC030679, NM_001081110, NM_031538, NM_001768
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Flow Cytometry (F), Cytotoxicity Assay (CT), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Functional assay (FN)
Background of CD8 antibody
The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either α and β or γ and δ chains. The vast majority of circulating T cells (95%) express the α/β heterodimer while roughly 2-5% express the γ/δ heterodimer.
CD3 chains and the CD4 or CD8 coreceptors are also required for efficient signal transduction through the TCR. The TCR is expressed on T helper and T cytotoxic cells that can be distinguished by their expression of CD4 and CD8. T helper cells express CD4 proteins and T cytotoxic cells display CD8. CD4 is also expressed on cortical cells, mature medullary thymocytes, microglial cells and dendritic cells. CD4, also designated T4 and Leu 3, is a 55 kDa membrane glycoprotein that contains four extracellular immunoglobinlike domains. The TCR, in association with CD4, can bind class II MHC molecules presented by the antigen-presenting cells. The CD4 protein functions by increasing the avidity of the interaction between the TCR and an antigen-class II MHC complex.
General readings
1. Zarkesh-Esfahani, H. et al. (2001) High-dose Leptin activates human leukocytes via receptor expression on monocytes. J. Immunol. 167: 4593 - 4599.
2. Manninen, A. et al. (2002) HIV-1 Nef interacts with inositol trisphosphate receptor to activate calcium signaling in T cells. J. Exp.Med. 195 (8): 1023 -1032.
3. Parnes, J.R. (1989) Molecular biology and function of CD4 and CD8. Adv. Immunol. 44: 265-311.
4. Kap, Y.S. et al. (2009) A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates. J Histochem Cytochem. 57: 1159-67.
5. Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses. BMC Immunol. 12: 2.
6. Nelson, M. et al. (2010) Characterization of lethal inhalational infection with Francisella tularensis in the common marmoset (Callithrix jacchus). J Med Microbiol. 59: 1107-13.
7. Gibbings, D.J. et al. (2007) CD8 alpha is expressed by human monocytes and enhances Fc gamma R-dependent responses.
BMC Immunol. 8: 12.
8. Junker, A. et al. (2007) Multiple sclerosis: T-cell receptor expression in distinct brain regions. Brain. 130: 2789-99.
9. Held, K. et al. (2011) Expression of HSV-1 encoded miRNAs in human trigeminal ganglia and their relation to local T-cell infiltrates. J Virol. Jul 27. [Epub ahead of print]
Comparison of CD4 and CD8 Monoclonal antibodies performed by flow cytometry
CYTOTOXICITY ANALYSIS - Antibody Titration
FLOW CYTOMETRY ANALYSIS - Tissue Distribution
Cell Source: CD3+ Spleen cells Percentage of cells stained above control: 13.1%
Representative Dot Plot
Representative Dot Plot
Representative Histogram - Isotypic Control: PE Rat IgG2a Percentage of cells stained above control: 13.04%
Representative Histogram - Isotypic Control: PE Rat IgG2a - Percentage of cells stained above control: 13.04%
Antibody Titration by Cytotoxicity Analysis
STRAIN DISTRIBUTION - Antibody Concentration: 1:500
Cytotoxic Index (C. I.) can be calculated as follows:
Flow Cytometry Analysis - Cell Source: Thymus - Percentage of cells stained above control: 85.2%
CYTOTOXICITY ANALYSIS - Antibody Titration
Cytotoxic Index
Surface staining of human peripheral blood cells with anti-human CD8 (MEM-31) FITC.
Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD8 (MEM-31) Sm152. Gated on singlets.
Surface staining of human peripheral blood lymphocytes using anti-human CD8 (clone MEM-31) PE.
Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) biotin.
Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) FITC.
Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) PerCP.
Surface staining of human peripheral blood lymphocytes using anti-human CD8 (clone MEM-31) PE
Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD8 (MEM-31) Sm152. Gated on singlets.
Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) biotin.
Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) FITC.
Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) PerCP.
Surface staining of human peripheral blood cells with anti-human CD8 (MEM-31) FITC.
Surface staining of human peripheral blood lymphocytes using anti-human CD8 (clone MEM-31) PE
Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD8 (MEM-31) Sm152. Gated on singlets.
Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) PerCP.
Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) biotin.
Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) FITC.
Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD8 antibody, clone YTS169.4 Cat.-No CL007P) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody. Medium power.
Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD8 antibody, clone YTS169.4 (Cat.-No CL007P) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody. Low power.
Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD8 antibody, clone YTS169.4 (Cat.-No CL007P) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody. High power.
CL007P (RAT ANTI MOUSE CD8) staining of mouse peripheral blood lymphocytes visualised with F(ab')2 RABBIT ANTI RATIgG: FITC.
Staining of mouse spleen with RAT ANTI MOUSE CD8 ALPHA: FITC
Staining of Mouse spleen cells with Rat anti Mouse CD8 antibody (SM011PS).
Staining of Mouse spleen cells with Rat Anti Mouse CD8 ALPHA-RPE
Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD8:FITC (SM1746F).
Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD8: AZIDE FREE (SM1784A).
CD8A polyclonal antibody (A01), Lot # 050912JC01. Western Blot analysis of CD8A expression in Daoy.
CD8A polyclonal antibody (A01), Lot # ABNOVA060619QCS1. Western Blot analysis of CD8A expression in SJCRH30.
Staining of sheep peripheral blood lymphocytes with MOUSE ANTI OVINE CD8:FITC (SM2080R).
Staining of human peripheral blood lymphocytes with Mouse Anti Human CD8-APC (SM1784APC).
Staining of canine peripheral blood lymphocytes with RAT ANTI CANINE CD8:FITC (SM411F).
Surface staining of human peripheral blood lymphocytes using anti-human CD8 (clone MEM-31) PE
Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD8 (MEM-31) Sm152. Gated on singlets.
Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) PerCP.
Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) biotin.
Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) FITC.
Surface staining of human peripheral blood lymphocytes using anti-human CD8 (clone MEM-31) PE
Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD8 (MEM-31) Sm152. Gated on singlets.
Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) PerCP.
Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) biotin.
Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) FITC.
Figure 1. Peripheral blood lymphocytes stained with AM05173RP-N antibody.
CD8 - AM05530SU-T - Tonsil. Formalin fixed, paraffin processed tissue. Mag. 40X.
AM05901FC-N staining of bovine peripheral blood lymphocytes.
Western blot analysis of CD8A ( arrow ) using rabbit CD8A polyclonal antibody ( Cat # PAB2495 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the CD8A gene ( Lane 2 ) ( Origene Technologies ) .
Western blot analysis of CD8A polyclonal antibody ( Cat # PAB2495 ) in Hela cell line lysates ( 35 µg/lane ) . CD8A ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD8A polyclonal antibody ( Cat # PAB2496 ) at a 1 : 50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of CD8A ( arrow ) using rabbit CD8A polyclonal antibody ( Cat # PAB2496 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the CD8A gene ( Lane 2 ) ( Origene Technologies ) .
Detection limit for recombinant GST tagged CD8A is approximately 3ng/ml as a capture antibody.
CD8A MaxPab polyclonal antibody. Western Blot analysis of CD8A expression in human spleen.
Western Blot analysis of CD8A expression in transfected 293T cell line (H00000925-T01) by CD8A MaxPab polyclonal antibody.Lane 1: CD8A transfected lysate(25.85 KDa).Lane 2: Non-transfected lysate.
Immunofluorescent Staining: Cells from BALB/c mesenteric lymph nodes were double-stained with anti-mouse CD8a-FITC and anti Mouse CD4-R-PE (clone GK1.5), gated on small lymphocytes and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount used: 1 µg/10e6 cells
Human tonsil stained with Anti-CD8 antibody (Cat# AP15454PU).
Human Spleen stained with anti-CD8 antibody
Human Cervix stained with anti-CD8 antibody
Human Endometrium stained with anti-CD8 antibody
Human tonsil stained with anti-CD8 antibody
Immunofluorescent Staining: Peripheral blood mononuclear cells were isolated from heparinized pig blood on Ficoll-Hypaque density gradients and double stained with Mouse anti-Pig CD8a-FITC and Mouse anti-Pig CD4a-PE. Lymphocytes were gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose,CA). Amount Used: 1 µg/10e6 cells.
Immunofluorescent Staining: Peripheral blood mononuclear cells from a healthy domestic Cat were double-stained with Mouse anti-Feline CD5-FITC (Cat.AM08111FC-N) and Mouse anti-Feline CD8-PE. Small lymphocytes were then gated and analyzed by two-color flow cytometry on a FACScan(TM) flow cytometry (BDIS, San Jose, CA). Amount Used: 0.2 µg/10e6 cells.
Immunofluorescent Staining: Chicken PBMC were double stained with Mouse anti-Chicken CD4-FITC and mouse anti-chicken CD8α-R-PE. Small lymphocytes were then gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 0.2 µg/10e6 cells.
Immunofluorescent Staining: Chicken peripheral blood mononuclear cells were double stained with Mouse anti-Chicken CD8a-FITC and Mouse anti-Chicken CD4-PE. Small lymphocytes were gated using light scatter criteria and analyzed on a FACScan(TM) flow cytometer (BDB, San Jose, CA). Amount Used: 1 µg/10e6 cells.
Thymus: Formalin-Fixed Paraffin-Embedded (FFPE)
Western Blot analysis of CD8A expression in transfected 293T cell line (H00000925-T02) by CD8A MaxPab polyclonal antibody.Lane 1: CD8A transfected lysate(25.70 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical staining of CD8A on formalin fixed, paraffin embedded human tonsil with CD8A polyclonal antibody.
Surface staining of human peripheral blood cells with CD8 monoclonal antibody, clone MEM-31 ( Cat # MAB3844 ) conjugated with FITC.
Cells from BALB/c mesenteric lymph nodes were double-stained with Cd8a monoclonal antibody-FITC and anti-mouse CD4-R-PE, gated on small lymphocytes and analyzed on a FACScanTM flow cytometer ( BDIS, San Jose, CA ).
Human Lung; WB Suggested Anti-CD8A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Lung; CD8A antibody - middle region (ARP59114_P050) in Human Lung cells using Western Blot
Human 721_B; WB Suggested Anti-CD8A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate; CD8A antibody - middle region (ARP59113_P050) in Human 721_B cells using Western Blot
Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 μm) stained with anti-CD8 antibody (DB 085) shows strong membranous immunostaining of T-lymphocytes. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic.
Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 μm) stained with anti-CD8 antibody (DB 085) shows strong membranous immunostaining of T-lymphocytes. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic.
Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 μm) stained with anti-CD8 antibody (DB 085) shows strong membranous immunostaining of T-lymphocytes. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic.
Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 μm) stained with anti-CD8 antibody (DB 085) shows strong membranous immunostaining of T-lymphocytes. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic.
A typical staining pattern with the B-Z31 monoclonal antibody of lymphocytes
A typical staining pattern with the B-Z31 monoclonal antibody of lymphocytes
A typical staining pattern with the B-Z31 monoclonal antibody of lymphocytes
A typical staining pattern with the B-Z31 monoclonal antibody of lymphocytes
FACS staining: expression on JY transfected cells.
Cell Source: Thymus. Percentage of cells stained above control: 85.2%
Cell Source: Thymus. Percentage of cells stained above control: 66.0%
Staining with clone MCD8 (CD8) monoclonal antibody is illustrated by flow cytometry analysis of normal blood cells. Direct staining was performed using 10 µl of the FITC-conjugated antibody and 100 µl blood sample.Testing by flow cytometry using a 'lyse-wash' method on whole blood from healthy donors showed the following values expressed in terms of % of the total lymphocyte count:Product code: AM39011FC-N (anti-CD8 FITC)n: 10Mean % positive: 26,21S.D.: 4,55% CV: 17,36
Flow Cytometric analysis of blood T cells using CD8 antibody Cat.-No AM06078PU-N (M2) and Negative Control (M1).
Bovine lymph node stained with Mouse anti Bovine CD8 Antibody Cat.-No AM05901PU-S (Clone CC63).
10x. Image courtesy of Dr. J.B. Silvan, Moredun Research Institute, Roslin, UK
10x. Image courtesy of Dr. J.B. Silvan, Moredun Research Institute, Roslin, UK
Bovine lymph node stained with Mouse anti Bovine CD8 Antibody Cat.-No AM05901PU-S (Clone CC63).
40x. Image courtesy of Dr. J.B. Silvan, Moredun Research Institute, Roslin, UK
40x. Image courtesy of Dr. J.B. Silvan, Moredun Research Institute, Roslin, UK
Flow Cytometry: BALB/c Mouse splenocytes were stained with a 1/8000 dilution of anti-Mouse CD8 Antibody Cat.-No AM32706SU-N (Clone 2.43) (purple) or negative control (green), followed by PE-conjugated Goat anti-Rat Ig (1/200 dilution).
Formalin-Fixed, Paraffin-Embedded Human tonsil (20X) stained with CD8 Antibody Cat.-No AM32847SU (Clone C8/468) at 1/200 dilution using peroxidase-conjugate and DAB chromogen.
Formalin-Fixed, Paraffin-Embedded Human tonsil (10X) stained with CD8 Antibody Cat.-No AM32847SU (Clone C8/468) at 1/200 dilution using peroxidase-conjugate and DAB chromogen.
Formalin-Fixed, Paraffin-Embedded Human tonsil tissue stained with CD8a Antibody Cat.-No AP32835PU using peroxidase-conjugate and DAB chromogen.
Surface staining of human peripheral blood lymphocytes using anti-human CD8 PE (SM3102R).
Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD8 (MEM-31) Sm152. Gated on singlets.
Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) PerCP.
Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) biotin.
Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) FITC.
Staining of Equine peripheral blood lymphocytes with Mouse anti Horse CD8 antibody Cat.-No SM2207P (Clone CVS8) followed by HRP conjugated Goat anti Mouse IgG HRP (Cat.-No AP05383FC-N).
Staining of Equine peripheral blood lymphocytes with Mouse anti Horse CD8 antibody PE conjugated Cat.-No SM2207R (Clone CVS8)
Staining of Equine peripheral blood lymphocytes with Mouse anti Horse CD8 antibody FITC conjugated Cat.-No SM2207F (Clone CVS8).
Formalin-Fixed, Paraffin-Embedded Human tonsil (20X) stained with CD8 Antibody Cat.-No AM50192PU (Clone SPM548). Note Cell surface staining.
Formalin-Fixed, Paraffin-Embedded Human tonsil (20X) stained with CD8 Antibody Cat.-No AM32847PU (Clone C8/468). Note Cell surface staining.
Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD8 Antibody Cat.-No AM10073PU (Clone C8/144B). Note Cell surface staining.
Formalin-Fixed, Paraffin-Embedded Human tonsil (20x) stained with CD8 Antibody Cat.-No AM33320PU (Clone C8/144B+C8/144B). Note Cell surface staining.
Western blot analysis of CD8a expression in A549 (A); Raw264.7 (B); PC12 (C) whole cell lysates.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD8 Antibody-Biotin labled; followed by Streptavidin PE.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD8 Antibody-APC labled.
Flow cytometric analysis of mouse spleen cells using Anti-CD8 Antibody-APC labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD8 Antibody-FITC labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD8 Antibody-FITC labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD8 Antibody-PE labled.
Flow cytometric analysis of mouse spleen cells using Anti-CD8 Antibody-PE labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD8 Antibody-PE-Cy5 labled.
Flow cytometric analysis of mouse spleen cells using Anti-CD8 Antibody-PE-Cy5 labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD8 Antibody; followed by anti-mouse IgG PE.
Flow cytometric analysis of mouse spleen cells using Anti-CD8 Antibody; followed by anti-mouse IgG PE.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-CD8A Rabbit Polyclonal antibody. (TA590306)
Western Blot: CD8 alpha Antibody - Western Blot was performed using affinity purified SEQer CD8a antibody, aa(71-170) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer CD8a antibody, aa(71-170) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD8A (RC213165, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD8A.
ELISA: CD8 alpha Antibody
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD8A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802079)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD8A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802079)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD8A (RC206608, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD8A.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD8A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802376)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD8A (RC206608, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD8A.
Western blot analysis of CD8A(arrow) using rabbit polyclonal CD8A Antibody (C-term) (Cat.#TA302179). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD8A gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD8A antibody (C-term) (Cat.#TA302179), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of NCI-H292 cells using CD8A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of anti-CD8A Antibody (N-term) (Cat.#TA302180) in Hela cell line lysates (35ug/lane). CD8A(arrow) was detected using the purified Pab.
Western blot analysis of CD8A(arrow) using rabbit polyclonal CD8A Antibody (N-term) (Cat.#TA302180). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD8A gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human tonsil tissue reacted with CD8A Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Staining of C57Bl/6 splenocytes with 0.125 ug of Rat IgG2a Isotype Control Purified (open histogram) or 0.125 ug of Anti-Mouse CD8 Purified (filled histogram) followed by Anti-Rat IgG FITC . Total viable cells were used for analysis.
Staining of normal human peripheral blood cells with Anti-Human CD8a FITC (left) and PE (right). Autofluorescence is indicated by open histogram. Cells in the lymphocyte population were used for analysis.
Immunohistochemistry staining of Paraffin Tonsil tissue by CD8 antibody (dilution: 1:25 - 1:100; visualization of staining: Membranous)
Western blot analysis of CD8A expression in Jurkat cells
Human peripheral blood lymphocytes stained with purified HIT8a, followed by anti - mouse IgGs FITC
C57BL/6 mouse splenocytes were stained with purified CD8 (clone 53-6.7) (filled histogram) or rat IgG2a, kappa; isotype control (open histogram), followed by anti-rat IgG FITC.
Human peripheral blood lymphocytes stained with purified RPA-T8, followed by anti-mouse IgGs FITC
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM05901PU-S | CD8 antibody |
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Mouse | IgG2a | CC63 | Purified | Bov, Gt, Sh | C, F, ICC/IF, IP, P |
0.1 mg /
€430.00
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
Published recently! |
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| AM10073PU-S | CD8 (C-term) antibody |
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Mouse | IgG1 | C8/144B | Purified | Hu | C, F, ICC/IF, IP, P, WB |
0.1 mg /
€430.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
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| AM10073PU-T | CD8 (C-term) antibody |
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|
Mouse | IgG1 | C8/144B | Purified | Hu | C, F, ICC/IF, IP, P, WB |
20 µg /
€240.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
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| AM32847PU-S | CD8 antibody |
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 |
Mouse | IgG1 | C8/468 | Purified | Hu | C, E, F, ICC/IF, IP, P, WB |
0.1 mg /
€430.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
|||||||
| AM32847PU-T | CD8 antibody |
||||||
|
Mouse | IgG1 | C8/468 | Purified | Hu | C, F, ICC/IF, IP, P, WB |
20 µg /
€240.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
|||||||
| AM33320PU-S | CD8 antibody |
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 |
Mouse | IgG1 | C8/468 + C8/144B | Purified | Hu | C, F, ICC/IF, IP, P, WB |
0.1 mg /
€430.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
|||||||
| AM33320PU-T | CD8 antibody |
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|
Mouse | IgG1 | C8/468 + C8/144B | Purified | Hu | C, F, ICC/IF, IP, P, WB |
20 µg /
€240.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
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| AM50192PU-S | CD8 antibody |
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 |
Mouse | IgG1 | SPM548 | Purified | Hu | C, F, ICC/IF, IP, P, WB |
0.1 mg /
€430.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
|||||||
| AM50192PU-T | CD8 antibody |
||||||
|
Mouse | IgG1 | SPM548 | Purified | Hu | C, F, ICC/IF, IP, P, WB |
20 µg /
€240.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
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| AM32411SU-N | CD8 antibody |
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Mouse | IgG3 | FK18 | Liquid | Hu, Mky | C, ICC/IF |
1 ml /
€220.00
|
| OriGene Technologies GmbH | |||||||
| AM32706SU-N | CD8 antibody |
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Rat | IgG2b | 2.43 | Ascites | Ms | F, ICC/IF |
0.1 ml /
€210.00
|
| OriGene Technologies GmbH | |||||||
| SM011P | CD8 antibody |
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Rat | IgG2a | KT15 | Purified | Ms | C, F, ICC/IF |
0.25 mg /
€460.00
|
| OriGene Technologies GmbH | |||||||
| SM011PS | CD8 antibody |
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 |
Rat | IgG2a | KT15 | Purified | Ms | C, F, ICC/IF |
0.1 mg /
€330.00
|
| OriGene Technologies GmbH | |||||||
| SM011PT | CD8 antibody |
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|
Rat | IgG2a | KT15 | Purified | Ms | C, F, ICC/IF |
25 µg /
€180.00
|
| OriGene Technologies GmbH | |||||||
| SM259P | CD8 antibody |
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Mouse | IgG1 | OX-8 | Purified | Rt | C, F, ICC/IF, IP, P, WB |
0.25 mg /
€460.00
|
| OriGene Technologies GmbH | |||||||
| SM259PX | CD8 antibody |
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|
Mouse | IgG1 | OX-8 | Purified | Rt | C, F, ICC/IF, IP, P, WB |
1 mg /
€720.00
|
| OriGene Technologies GmbH | |||||||
