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CD8 beta antibody

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Principal name

CD8 beta antibody

Alternative names for CD8 beta antibody

CD8B, CD8B1, T-cell surface glycoprotein CD8 beta chain

SwissProt ID

P05541 (Rat), P10300 (Mouse), P10966 (Human), P79336 (Felca), Q90769 (Chick)

Gene ID

12526 (Cd8b1), 926 (CD8B), 396175, 24931 (Cd8b)

Ncbi ID

NP_033988.1, NP_001171571.1, NP_990578, NP_113727.1, 9606, NP_004922, NP_033988, NM_004931, NP_113727, NP_757362.1, NM_172213, NM_001178100, NP_001171571, NM_009858, NM_031539

Available reactivities

Ms (Mouse), Hu (Human), Mky (Monkey), Duck, Rt (Rat), Bov (Bovine), Av (Birds), Chk (Chicken), Gt (Goat), Sh (Sheep), Can (Canine), Rb (Rabbit), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rat, Rabbit

Available applications

Cytotoxicity Assay (CT), Flow Cytometry (F), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Frozen Sections (C), Paraffin Sections (P)

Background of CD8 beta antibody

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. CD8B is the CD8 beta chain isoforms.The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.

General readings

1. Terry, L.A. et al. (1990) Differential expression and regulation of the human CD8 alpha and CD8 beta chains. Tissue Antigens 35: 82-91.
2. DiSanto, J.P. et al. (1991) Generation of anti-human CD8 beta specific antibodies using transfectants expressing mixed species CD8 heterodimers. J. Immunol. Methods 141: 123-131.
3. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49: 97-110.
4. Fry, T.J. et al. (2002) IL-7 therapy dramatically alters peripheral T-cell homeostasis in normal and SIV-infected nonhuman primates.
Blood. 101: 2294-9.
5. Schmitz, J.E. et al (1998) Expression of the CD8alpha beta-heterodimer on CD8(+) T lymphocytes in peripheral blood lymphocytes of human immunodeficiency virus- and human immunodeficiency virus+ individuals.
Blood. 92: 198-206.

CL011 - CD8 beta
Tissue Distribution by Cytotoxicity Analysis
CL011 - CD8 beta
Strain Distribution by Cytotoxicity Analysis
CL011 - CD8 beta
Antibody Titration by Cytotoxicity Analysis
CL011 - CD8 beta
Cytotoxic index
CL012B - CD8 beta
Flow Cytometry - Representative histogram - Cell Source: Spleen - Percentage of cells stained above control: 9.2%
CL012BX - CD8 beta
Flow Cytometry - Cell Source: Spleen - Percentage of cells stained above control: 9.2%
CL012P - CD8 beta
Cell Source: Balb/c thymus labelled with CL012P including isotypic control. Percentage of cells stained above control: 90.9%
SM439R - CD8 beta
SM439R (MOUSE ANTI BOVINE CD8 BETA:RPE) staining of bovine peripheral blood lymphocytes.
H00000926-B01 - CD8 beta
Western Blot analysis of CD8B expression in transfected 293T cell line (H00000926-T01) by CD8B MaxPab polyclonal antibody.Lane 1: CD8B1 transfected lysate(26.73 KDa).Lane 2: Non-transfected lysate.
PAB3088 - CD8 beta
Western blot analysis of CD8B polyclonal antibody ( Cat # PAB3088 ) in K-562 cell line lysates ( 35 µg/lane ) . CD8B ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
ARP42204_P050 - CD8 beta
Human Jurkat; WB Suggested Anti-CD8B Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; CD8B antibody - N-terminal region (ARP42204_P050) in Human Jurkat cells using Western Blot
AP11413PU-N - CD8 beta
Western blot analysis of anti-CD8B1 Pab (AP11413PU-N) in K562 cell line lysates (35 µg/lane). CD8B1 (arrow) was detected using the purified Pab (1:60 dilution).
AM08123FC-N - CD8 beta
Immunofluorescent Staining: Chicken IEL were double stained with Mouse anti-Chicken CD8b-FITC and Mouse anti-Chicken CD8b-RPE. Small lymphocytes were then gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 µg/10e6 cells.
H00000926-B01P - CD8 beta
Western Blot analysis of CD8B expression in transfected 293T cell line (H00000926-T02) by CD8B MaxPab polyclonal antibody.Lane 1: CD8B1 transfected lysate(26.73 KDa).Lane 2: Non-transfected lysate.
H00000926-D01P - CD8 beta
Western Blot analysis of CD8B expression in transfected 293T cell line (H00000926-T02) by CD8B MaxPab polyclonal antibody.Lane 1: CD8B transfected lysate(27.20 KDa).Lane 2: Non-transfected lysate.
H00000926-D01 - CD8 beta
Western Blot analysis of CD8B expression in transfected 293T cell line (H00000926-T02) by CD8B MaxPab polyclonal antibody.Lane 1: CD8B transfected lysate(27.20 KDa).Lane 2: Non-transfected lysate.
H00000926-D01 - CD8 beta
Immunoprecipitation of CD8B transfected lysate using anti-CD8B MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CD8B MaxPab mouse polyclonal antibody (B01) (H00000926-B01).
ARP42715_P050 - CD8 beta
Human ACHN; WB Suggested Anti-CD8B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: ACHN cell lysate; CD8B antibody - middle region (ARP42715_P050) in Human ACHN cells using Western Blot
ARP42205_P050 - CD8 beta
Human HT1080; WB Suggested Anti-CD8B Antibody Titration: 0.2-1 ug/ml. Positive Control: HT1080 cell lysate; CD8B antibody - middle region (ARP42205_P050) in Human HT1080 cells using Western Blot
SM260B - CD8 beta
Cell Source: Thymus. Percentage of cells stained above control: 90.0%. Representative Histogram
SM260FX - CD8 beta
Cell Source: Thymus. Percentage of cells stained above control: 90.0%
SM260RX - CD8 beta
Cell Source: Thymus. Percentage of cells stained above control: 92.2%
AR51340PU-N - CD8 beta
CPA2941-100ul - CD8 beta
Western blot analysis of CD8b expression in Jurkat (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
TP720467 - CD8 beta
TA347884 - CD8 beta
Western blot analysis of CD8B expression in Jurkat cells
TA352395 - Cd8b
LOU rat splenocytes stained with purified 341, followed by anti-mouse IgG FITC
TA352381 - Cd8b1
Mouse OT-I primary CTL cells were stained with purified anti-mouse CD8b primary antibody at 1:50 dilution in PBS/1%NGS incubated O/N at 4 ?, detected with anti-rat IgG2b FITC at 1:2000 dilution for 1hr at room temperature (green), plus DAPI staining for nuclei (blue). Imaged with a Zeiss 200M deconvolution microscope (deconvolution applied). Negative control was anti-rat IgG2b isotype control plus secondary antibody, same conditions. Images courtesy of Dr. Javier Casas at The Scripps Research Institute.
TA352381 - Cd8b1
C57BL/6 mouse splenocytes stained with purified YTS156.7.7, followed by anti-rat IgG FITC
TA346387 - CD8 beta
WB Suggested Anti-CD8B Antibody Titration: 0.2-1 ug/ml; Positive Control: HT1080 cell lysate
TA346386 - CD8 beta
WB Suggested Anti-CD8B Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate
TA329512 - CD8 beta
WB Suggested Anti-CD8B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: ACHN cell lysate
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD8 beta antibody
CD8 beta Mouse IgG2a 2ST8.5H7 Purified Hu, Mky F, IP, WB
0.2 mg / €490.00
  OriGene Technologies GmbH
 

Published recently!

CD8 beta antibody
  Rabbit Polyclonal Anti-CD8B Antibody  
Western blot analysis of CD8B expression in Jurkat cells Rabbit IgG Purified Hu WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

CD8 beta antibody
CD8 beta Rat IgG2b YTS156.7.7 PE Ms F
0.5 ml / €850.00
  OriGene Technologies GmbH

CD8 beta antibody
CD8 beta Mouse IgG2a EP42 Biotin Chk F
0.5 mg / €450.00
  OriGene Technologies GmbH

CD8 beta antibody
Immunofluorescent Staining: Chicken IEL were double stained with Mouse anti-Chicken CD8b-FITC and Mouse anti-Chicken CD8b-RPE. Small lymphocytes were then gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 µg/10e6 cells. Mouse IgG2a EP42 FITC Chk F
0.5 mg / €500.00
  OriGene Technologies GmbH

CD8 beta antibody
CD8 beta Mouse IgG2a EP42 Purified Chk C, F, IP
0.5 mg / €370.00
  OriGene Technologies GmbH

CD8 beta antibody
CD8 beta Mouse IgG2a EP42 PE Chk F
0.1 mg / €340.00
  OriGene Technologies GmbH

CD8 beta (N-term) antibody
Western blot analysis of anti-CD8B1 Pab (AP11413PU-N) in K562 cell line lysates (35 µg/lane). CD8B1 (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

CD8 beta antibody
Tissue Distribution by Cytotoxicity Analysis Mouse IgM F9(1) Ascites Ms CT
0.5 ml / €520.00
  OriGene Technologies GmbH
+3 additional images

CD8 beta antibody
CD8 beta Mouse IgM F9(1) Supernatant Ms CT
1 ml / €190.00
  OriGene Technologies GmbH

CD8 beta antibody
Flow Cytometry - Representative histogram - Cell Source: Spleen - Percentage of cells stained above control: 9.2% Rat IgG2a CT-CD8b Biotin Ms F
0.1 mg / €300.00
  OriGene Technologies GmbH

CD8 beta antibody
Flow Cytometry - Cell Source: Spleen - Percentage of cells stained above control: 9.2% Rat IgG2a CT-CD8b Biotin Ms F
0.3 mg / €570.00
  OriGene Technologies GmbH

CD8 beta antibody
CD8 beta Rat IgG2a CT-CD8b FITC Ms F
0.1 mg / €300.00
  OriGene Technologies GmbH

CD8 beta antibody
CD8 beta Rat IgG2a CT-CD8b FITC Ms F
0.3 mg / €570.00
  OriGene Technologies GmbH

CD8 beta antibody
Cell Source: Balb/c thymus labelled with CL012P including isotypic control. Percentage of cells stained above control: 90.9% Rat IgG2a CT-CD8b Purified Ms F
0.2 mg / €380.00
  OriGene Technologies GmbH

CD8 beta antibody
CD8 beta Rat IgG2a CT-CD8b PE Ms F
50 µg / €320.00
  OriGene Technologies GmbH

CD8 beta antibody
CD8 beta Rat IgG2a CT-CD8b PE Ms F
0.3 mg / €890.00
  OriGene Technologies GmbH

CD8 beta antibody
Cell Source: Thymus. Percentage of cells stained above control: 90.0%. Representative Histogram Mouse IgG1 3.4.1 Biotin Rt F
0.1 mg / €260.00
  OriGene Technologies GmbH

CD8 beta antibody
Cell Source: Thymus. Percentage of cells stained above control: 90.0%. Representative Histogram Mouse IgG1 3.4.1 Biotin Rt F
0.5 mg / €490.00
  OriGene Technologies GmbH

CD8 beta antibody
CD8 beta Mouse IgG1 341 FITC Rt F
0.1 mg / €400.00
  OriGene Technologies GmbH

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