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CD82 antibody

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Principal name

CD82 antibody

Alternative names for CD82 antibody

KAI1, SAR2, ST6, TSPAN27, C33 antigen, IA4, Kangai-1, Tetraspanin-27, Inducible membrane protein R2, Metastasis suppressor Kangai-1, Suppressor of tumorigenicity protein 6

SwissProt ID

O70352 (Rat), P27701 (Human), P40237 (Mouse)

Gene ID

3732 (CD82), 12521 (Cd82)

Ncbi ID

9606, NP_002222, NP_001020015.1, NP_001020015, NM_002231, NP_001129527.1

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of CD82 antibody

KAI1 was initially identified from a T-cell activation study as a four-transmembrane protein that plays an accessory role in T-cell activation, and was later determined to act as a cancer metastasis suppressor gene. This protein is ubiquitously expressed at moderate to high levels in most tissues, but its expression is downregulated during tumor progression. The loss of KAI1 and p53 is associated with poor survival for prostate and other cancer patients. Recently, KAI1 was found to interact with DARC, the Duffy antigen for chemokines using a yeast two hybrid screen. It is thought that tumor cells dislodged from the primary tumor and expressing KAI1 interact with DARC proteins expressed on vascular cells, transmitting a senescent signal to the tumor cells, while tumor cells that have lost KAI1 expression can proliferate and potentially give rise to metastases. At least two isoforms of KAI1 are known to exist.

H00003732-M01 - CD82
Proximity Ligation Analysis (PLA) of protein-protein interactions between CD19 and CD82. HeLa cells were stained with anti-CD19 rabbit purified polyclonal 1:1200 and anti-CD82 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
H00003732-M01 - CD82
Detection limit for recombinant GST tagged CD82 is approximately 0.3ng/ml as a capture antibody.
PAB4830 - CD82
The CD82 polyclonal antibody ( Cat # PAB4830 ) is used in Western blot to detect CD82 in mouse liver tissue lysate.
PAB4830 - CD82
Formalin-fixed and paraffin-embedded human cancer tissue reacted with CD82 polyclonal antibody ( Cat # PAB4830 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
AP13224PU-N - CD82
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
AP13224PU-N - CD82
(LEFT)The anti-ST6 C-term Antibody is used in Western blot to detect ST6 in mouse liver lysate. (RIGHT)Western blot analysis of ST6 (arrow) using ST6 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD82 gene (Lane 2)
PAB12987 - CD82
Immunohistochemistry of CD82 in human colon tissue with CD82 polyclonal antibody ( Cat # PAB12987 ) at 2.5 µg/ml.
PAB12987 - CD82
Western blot analysis of CD82 in A-549 cell lysate with CD82 polyclonal antibody ( Cat # PAB12987 ) at ( A ) 0.5 and ( B ) 1 µg/ml.
PAB13682 - CD82
Western blot analysis of whole cell lysates with CD82 polyclonal antibody ( Cat # PAB13682 ).Lane 1 : MCF-7.Lane 2 : HeLa.Lane 3 : Jurkat.Lane 4 : Hmy2.
AP23396PU-N - CD82
Western blot with CD82 Polyclonal Antibody
AM31359PU-N - CD82
A typical staining pattern with the B-L2 monoclonal antibody of lymphocytes.
AM31359RP-N - CD82
A typical staining pattern with the B-L2 monoclonal antibody of lymphocytes.
AM31359AF-N - CD82
A typical staining pattern with the B-L2 monoclonal antibody of lymphocytes.
TA306407 - CD82
Western blot analysis of KAI1 in A549 cell lysate with KAI1 antibody at (A) 0.5 and (B) 1 ug/ml.
TA306407 - CD82
Immunohistochemistry of KAI1 in human colon tissue with KAI1 antibody at 2.5 ug/ml.
TA306407 - CD82
Immunofluorescence of KAI1 in Human Colon cells with KAI1 antibody at 20 ug/mL.
TA332430 - CD82
Western blot analysis of extracts of various cell lines, using CD82 antibody.
TA343300 - CD82
WB Suggested Anti-CD82 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Brain
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD82 antibody
CD82 Mouse IgG2a C33 Aff - Purified Hu F, FN, ICC/IF, IP, P, WB
0.1 mg / €230.00
  OriGene Technologies GmbH
 

Published recently!

CD82 (C-term) antibody
  Rabbit Polyclonal KAI1 Antibody  
Western blot analysis of KAI1 in A549 cell lysate with KAI1 antibody at (A) 0.5 and (B) 1 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

CD82 antibody
  Rabbit anti-CD82 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using CD82 antibody. Rabbit IgG Purified Hu
0.1 mg / €335.00
  OriGene Technologies, Inc.

CD82 antibody
Surface staining of CD82 on human peripheral blood cells with anti-CD82 (C33) APC. Mouse IgG2a C33 APC Hu F
100 Tests / €290.00
  OriGene Technologies GmbH

CD82 antibody
CD82 Mouse IgG2a C33 Biotin Hu F
0.1 mg / €260.00
  OriGene Technologies GmbH

CD82 antibody
CD82 Mouse IgG2a C33 Hu F
100 Tests / €260.00
  OriGene Technologies GmbH

CD82 antibody
CD82 Mouse IgG2a C33 PE Hu F
100 Tests / €290.00
  OriGene Technologies GmbH

CD82 antibody
A typical staining pattern with the B-L2 monoclonal antibody of lymphocytes. Mouse IgG1 B-L2 Azide Free Hu C, F
0.2 mg / €350.00
  OriGene Technologies GmbH

CD82 antibody
A typical staining pattern with the B-L2 monoclonal antibody of lymphocytes. Mouse IgG1 B-L2 Purified Hu C, F
2ml (200T) / €320.00
  OriGene Technologies GmbH

CD82 antibody
A typical staining pattern with the B-L2 monoclonal antibody of lymphocytes. Mouse IgG1 B-L2 PE F
1ml (100T) / €390.00
  OriGene Technologies GmbH

CD82 (C-term) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

CD82 (C-term) antibody
Western blot with CD82 Polyclonal Antibody Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €390.00
  OriGene Technologies GmbH

Lysates

Catalog No.    

CD82 overexpression lysate
CD82 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

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  1. Lysate (1)
  2. Primary Antibody (12)
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Property
  1. C-term (3)
Reactivity
  1. Human (Hu) (11)
  2. Mouse (Ms) (3)
  3. Rat (Rt) (2)
Applications
  1. Flow cytometry (F) (8)
  2. Western blot / Immunoblot (WB) (4)
  3. Paraffin Sections (P) (3)
  4. Frozen sections (C) (2)
  5. Enzyme Immunoassay (E) (1)
  6. Functional assay (FN) (1)
  7. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  8. Immunoprecipitation (IP) (1)
Presentation
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  2. 3+ images (1)
Images: Applications
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  2. Western blot / Immunoblot (WB) (4)
  3. Immunofluorescence (IF) (1)
  4. IHC (1)
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Manufacturer
  1. OriGene Technologies (13)
Service
  • OriGene Technologies GmbH
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