Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » CD86 antibody

CD86 antibody

(reset)

Principal name

CD86 antibody

Alternative names for CD86 antibody

CD28LG2, Activation B7-2 antigen, B70, BU63, B7.2, FUN-1, T-lymphocyte activation antigen CD86, CTLA-4 counter-receptor B7.2

SwissProt ID

O35531 (Rat), P42071 (Rabit), P42081 (Human), P42082 (Mouse)

Gene ID

12524 (Cd86), 942 (CD86), 56822

Ncbi ID

NP_062261.3, NP_008820.3, NP_064466.1, 9606, NP_062261, 10090, NP_008820, AAH40261.1, NM_020081, NM_019388, NM_006889, NM_001206924

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat)

Available hosts

Rat, Mouse, Rabbit

Available applications

Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of CD86 antibody

B7-1 and B7-2 are transmembrane glycoproteins of the immunoglobulin superfamily that are expressed, along with the receptors CD28 and CTLA4, by antigen-presenting cells, and along with these receptors, constitute crucial co-stimulatory pathways for T and B cell regulatory responses. As members of the B7 family, B7-1 and B7-2 play principal roles in immunity, activating immune response and maintaining immune tolerance through engagement with CD28 and CTLA4. Co-stimulatory signals generated by B7-1 and B7-2 interactions with CD28 serve to stimulate T cell activation and prevent anergy through the amplification of T cell receptor (TCR) signaling. In contrast, interactions of the ligands with CTLA-4 serves to maintain T cell homeostasis and self-tolerance through the disruption of stimulatory signaling from B7 isoform bound CD28 complexes, and by inducing powerful inhibitory signals in T cells. B7-1 plays an important role in immune response through its amplification and regulation of T cell activity at peripheral inflammation sites. B7-1, like CTLA-4, is, however, only poorly expressed on resting dendritic cells, and its up-regulation is, therefore, considerably delayed upon immune activation. Conversely, B7-2 and CD28 are constitutively expressed by resting hematopoietic and T cells, respectively, and as a result are able to rapidly induce up-regulation upon immune activation, making them critical to the early co-stimulatory signaling of immune response. Both B7-1 and B7-2 have been shown to demonstrate co-stimulatory activity in T-cell proliferation in vitro and elicit enhanced antitumor immune response in vivo.

General readings

1. Hathcock, K., G. Laszlo, C. Pucello, O. Linsley, and R.J. Hodes. 1994. J. Exp. Med. 180:631.
2. Freeman, G.J., F. Borriello, R.J. Hodes, H. Reiser, K.S. Hathcock, G. Laszlo, A.J. McKnight, J. Kim, L. Du, D.B. Lombard, G.S. Gray, L.M. Nadler,
and A.H. Sharpe. 1993. Science 262:907.
3. Inaba, K. M. Witmer-Pack, M. Inaba, K.S. Hathcock, H. Sakuta, et al. 1994. J. Exp. Med. 180:1849.
4. Larsen, C.P., S.C. Ritchie, R. Hendrix, P.S. Linsley, K.S. Hathcock, R.J. Hodes, R.P. Lowry, and T.C. Pearson. 1994. J. Immunol. 152:5208.
5. Hathcock, K.S., G. Laszlo, C. Pucillo, P. Linsley, and R.J. Hodes. 1994. J. Exp. Med. 180:631.
6. Laszlo, G., K.S. Hathcock, H.B. Dickler, and R.J. Hodes. 1993. J. Immunol. 150:5252.
7. Hathcock, K., G. Laszlo, H. Dickler, J. Bradshaw, P. Linsley, and R Hodes. 1993. Science 262:905.
8. Thompson, C.B. 1995. Cell 978:982.
9. June, C.H., J.A. Bluestone, L.M. Nadler, and C.B. Thompson. 1994. Immuol. Today 15:321.
10. Han, S. et. al. 1995. J. Immunol. 155:556.

SM1167F - CD86
Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD86:FITC (SM1167F).
SM1167R - CD86
Staining of human peripheral blood monocytes with Mouse Anti Human CD86-RPE (SM1167R).
SM1167RT - CD86
Staining of human peripheral blood monocytes with Mouse Anti Human CD86-RPE (SM1167RT).
H00000942-D01P - CD86
Proximity Ligation Analysis (PLA) of protein-protein interactions between CD86 and CD80. HeLa cells were stained with anti-CD86 rabbit purified polyclonal 1:1200 and anti-CD80 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
H00000942-D01P - CD86
Western Blot analysis of CD86 expression in transfected 293T cell line (H00000942-T01) by CD86 MaxPab polyclonal antibody.Lane 1: CD86 transfected lysate(37.70 KDa).Lane 2: Non-transfected lysate.
AM08053FC-N - CD86
Immunofluorescent Staining: BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with Rat anti-Mouse CD86-FITC. A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 1 µg/10e6 cells.
AM08084FC-N - CD86
Immunofluorescent Staining: BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with anti-Mouse CD86-FITC. A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 1 µg/10e6 cells.
AM08084FC-N - CD86
BA/2 Mouse mastocytoma cell line P815 and mB7.2-Ig transfected P815 cells were stained with Rat anti-Mouse CD86 Antibody cat.-No AM08084FC-N
MAB3238 - CD86
BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with Cd86 monoclonal antibody, clone GL1 ( Cat # MAB3238 )-FITC. A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScanTM flow cytometer ( BDIS, San Jose, CA ).
MAB3846 - CD86
Surface staining of human peripheral blood cells with CD86 monoclonal antibody, clone BU63 ( Cat # MAB3846 ) - PerCP ( monocyte gate ).
MAB5112 - CD86
Surface staining of human peripheral blood cells with CD86 monoclonal antibody, clone BU63 (PerCP) ( Cat # MAB5112 ).
AM31241AF-N - CD86
A typical staining pattern with the B-T7 monoclonal antibody of monocytes
AM31241PU-N - CD86
A typical staining pattern with the B-T7 monoclonal antibody of monocytes
AM31241FC-N - CD86
A typical staining pattern with the B-T7 monoclonal antibody of monocytes
AM31241RP-N - CD86
A typical staining pattern with the B-T7 monoclonal antibody of monocytes
AM06519SU-N - CD86
Western blot analysis using CD86 antibody Cat.-No AM06519SU-N against L1210 (1) and MOLT-4 (2) cell lysate.
SM1167PP - CD86
Surface staining of human peripheral blood cells with anti-CD86 PerCP (monocyte gate), SM1167PP.
CEK1435 - Mouse CD86 ELISA Kit
Representative Standard Curve
CPA8395-50Assays - CD86
Flow cytometric analysis of Daudi cells using Anti-CD86 Antibody-FITC labled.
CPA8465-50Assays - CD86
Flow cytometric analysis of Daudi cells using Anti-CD86 Antibody-PE labled.
CPA8559-100ul - CD86
Flow cytometric analysis of Daudi cells using Anti-CD86 Antibody; followed by anti-mouse IgG PE.
TP720346 - CD86
TA320295 - CD86
Surface staining of LPS-stimulated rat splenocytes with Anti-Rat CD86 (B7-2) FITC (left) and PE (right). Autofluorescence is shown via open histogram. Total viable cells were used for analysis.
TA320296 - CD86
Staining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.25 ug of Purified Anti-Mouse CD86 (B7-2) (filled histogram) followed by Anti-Rat IgG FITC . Total viable cells were used for analysis.
TA320298 - CD86
Staining of 2-day unstimulated (left) or LPS-stimulated (right) C57BL/6 splenocytes with 0.25 ug of Rat IgG2b K Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD86 (B7-2) Purified (filled histogram) followed by Anti-Rat IgG FITC . Total viable cells were used for analysis.
TA326991 - CD86
Western blot analysis of extracts of various cell lines,using CD86 antibody.
TA326991 - CD86
Immunofluorescence analysis of MCF-7 cell using CD86 antibody. Blue: DAPI for nuclear staining.
TA352358 - CD86
LPS-stimulated (2 days) C57BL/6 mouse splenocytes stained with purified PO3, followed by anti-rat IgG FITC

Items 1 to 20 of 39 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD86 antibody
  Rat Anti-Mouse CD86 (B7-2) Purified (100 ug)  
Staining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.25 ug of Purified Anti-Mouse CD86 (B7-2) (filled histogram) followed by Anti-Rat IgG FITC . Total viable cells were used for analysis. Rat GL1 Purified Ms F, IP, P
0.1 mg / €195.00
  OriGene Technologies, Inc.

CD86 antibody
  Rat Anti-Mouse CD86 (B7-2) Purified (100 ug)  
Staining of 2-day unstimulated (left) or LPS-stimulated (right) C57BL/6 splenocytes with 0.25 ug of Rat IgG2b K Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD86 (B7-2) Purified (filled histogram) followed by Anti-Rat IgG FITC . Total viable cells were used for analysis. Rat PO3.1 Purified Ms F
0.1 mg / €195.00
  OriGene Technologies, Inc.

CD86 antibody
  CD86 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines,using CD86 antibody. Rabbit IgG Purified Hu, Ms WB
0.1 mg / €335.00
  OriGene Technologies, Inc.
+1 additional image

CD86 antibody
CD86 Rat IgG2b PO3 FITC Ms F
0.1 mg / €400.00
  OriGene Technologies GmbH

CD86 antibody
CD86 Rat IgG2b PO3 PE Ms F
100 Tests / €480.00
  OriGene Technologies GmbH

CD86 antibody
CD86 Rat IgG2b PO3 PE Ms F
25 Tests / €220.00
  OriGene Technologies GmbH

CD86 antibody
Western blot analysis using CD86 antibody Cat.-No AM06519SU-N against L1210 (1) and MOLT-4 (2) cell lysate. Mouse IgG1 1B3 Ascites Hu E, WB
0.1 ml / €380.00
  OriGene Technologies GmbH

CD86 antibody
CD86 Rat IgG2a GL1 APC Ms F
0.1 mg / €360.00
  OriGene Technologies GmbH

CD86 antibody
CD86 Rat IgG2a GL1 Biotin Ms F
0.5 mg / €330.00
  OriGene Technologies GmbH

CD86 antibody
Immunofluorescent Staining: BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with Rat anti-Mouse CD86-FITC. A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 1 µg/10e6 cells. Rat IgG2a GL1 FITC Ms F
0.5 mg / €360.00
  OriGene Technologies GmbH

CD86 antibody
CD86 Rat IgG2a GL1 Low Endotoxin Ms F
0.5 mg / €380.00
  OriGene Technologies GmbH

CD86 antibody
CD86 Rat IgG2a GL1 Purified Ms C, F, FN, IP
0.5 mg / €310.00
  OriGene Technologies GmbH

CD86 antibody
CD86 Rat IgG2a GL1 PE-Cy7 Ms F
0.1 mg / €360.00
  OriGene Technologies GmbH

CD86 antibody
CD86 Rat IgG2a GL1 PE Ms F
0.2 mg / €380.00
  OriGene Technologies GmbH

CD86 antibody
CD86 Rat IgG2a GL1 PE Ms F
0.1 mg / €300.00
  OriGene Technologies GmbH

CD86 antibody
CD86 Rat IgG2b 2D10 APC Ms F
0.1 mg / €360.00
  OriGene Technologies GmbH

CD86 antibody
CD86 Rat IgG2b 2D10 Biotin Ms C, F, IP
0.5 mg / €350.00
  OriGene Technologies GmbH

CD86 antibody
Immunofluorescent Staining: BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with anti-Mouse CD86-FITC. A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 1 µg/10e6 cells. Rat IgG2b 2D10 FITC Ms C, F
0.5 mg / €360.00
  OriGene Technologies GmbH
+1 additional image

CD86 antibody
CD86 Rat IgG2b 2D10 Low Endotoxin Ms C, F, FN, IP
0.5 mg / €380.00
  OriGene Technologies GmbH

CD86 antibody
CD86 Rat IgG2b 2D10 Purified Ms C, F, FN, IP
0.5 mg / €310.00
  OriGene Technologies GmbH

Items 1 to 20 of 39 total

per page
Page:
  1. 1
  2. 2
39 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. ELISA Kit (3)
  2. Primary Antibody (33)
  3. Protein | Growth Factor (1)
  4. Rat Lenti ORF Particle (2)
Clonality
  1. Monoclonal (32)
  2. Polyclonal (1)
Target
  1. CD86 (34)
Reactivity
  1. Mouse (Ms) (21)
  2. Human (Hu) (13)
  3. Rat (Rt) (1)
Applications
  1. Flow cytometry (F) (31)
  2. Frozen sections (C) (8)
  3. Immunoprecipitation (IP) (8)
  4. Functional assay (FN) (5)
  5. Western blot / Immunoblot (WB) (3)
  6. Enzyme Immunoassay (E) (1)
  7. Paraffin Sections (P) (1)
Presentation
  1. Purified (8)
  2. PE (7)
  3. FITC (6)
  4. Low Endotoxin (3)
  5. APC (2)
  6. Biotin (2)
  7. PE-Cy7 (2)
  8. Ascites (1)
  9. Azide Free (1)
  10. PerCP (1)
Host
  1. Rat (20)
  2. Mouse (12)
  3. Rabbit (1)
Images
  1. 1 image (15)
Images: Applications
  1. Flow cytometry (F) (10)
  2. Western blot / Immunoblot (WB) (2)
  3. Immunofluorescence (IF) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (39)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn