CDC6 antibody
Principal name
CDC6 antibody
Alternative names for CDC6 antibody
CDC18L, CDC6-related protein, p62(cdc6), HsCDC6, Cdc18-related protein, HsCdc18, Cell division control protein 6 homolog
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of CDC6 antibody
In yeasts, Cdc6 (Saccharomyces cerevisiae) and Cdc18 (Schizosaccharomyces pombe) associate with the origin recognition complex (ORC) proteins to render cells competent for DNA replication. Cdc6 is overexpressed in human cancers, where it has a critical regulatory role in addition to DNA replication. Yan et al. (1998) showed that Cdc6 is expressed selectively in proliferating but not quiescent mammalian cells, both in culture and within tissues in intact animals. During the transition from a growth-arrested to a proliferative state, transcription of mammalian Cdc6 is regulated by E2F proteins, as revealed by a functional analysis of the human Cdc6 promoter and by the ability of exogenously expressed E2F proteins to stimulate the endogenous Cdc6 gene. They conclude that expression of human Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using CDC6 antibody (Cat.-No. AP06045PU-N).
Western blot (WB) analysis of Cdc6 antibody (Cat.-No.: AP06045PU-N) at 1/500 dilution
Lane1:Hela cell lysate
Lane2:Raw264.7 cell lysate
Lane3:H9C2 cell lysate
Lane1:Hela cell lysate
Lane2:Raw264.7 cell lysate
Lane3:H9C2 cell lysate
Western blot (WB) analyzes of Cdc6 antibody (Cat.-No.: AP06778PU-N) in extracts from K562 cells..
Formalin-fixed and paraffin-embedded human cancer tissue reacted with CDC6 (phospho S54) polyclonal antibody ( Cat # PAB0435 ) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
The CDC6 (phospho S54) polyclonal antibody ( Cat # PAB0435 ) is used in Western blot to detect Phospho-CDC6-S54 in Ramos cell lysate
CDC6 (phospho S54) polyclonal antibody ( Cat # PAB0435 ). Western blot analysis of CDC6 expression in human colon.
CDC6 (phospho S54) polyclonal antibody ( Cat # PAB0435 ). Western blot analysis of CDC6 expression in HeLa.
Western blot analysis of CDC6 polyclonal antibody ( Cat # PAB4853 ) in Romas cell lysate ( 35 µg/lane ) . CDC6 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
The anti-Phospho-CDC6-S54 Pab (Cat. #AP12586PU-N) is used in Western blot to detect Phospho-CDC6-S54 in Ramos tissue lysate
Western blot analysis of Cdc6 Antibody (N-term) in Romas cell line lysates (35ug/lane). Cdc6 (arrow) was detected using the purified Pab (1:60 dilution).
Testis: Formalin-Fixed Paraffin-Embedded
Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC6 and PCNA. Mahlavu cells were stained with anti-CDC6 rabbit purified polyclonal 1:1200 and anti-PCNA mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC6 and MCM7. Huh7 cells were stained with anti-CDC6 rabbit purified polyclonal 1:600 and anti-MCM7 mouse monoclonal antibody 1:100. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC6 and CDKN1A. HeLa cells were stained with anti-CDC6 rabbit purified polyclonal 1:1200 and anti-CDKN1A mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of CDC6 expression in transfected 293T cell line (H00000990-T02) by CDC6 MaxPab polyclonal antibody.Lane 1: CDC6 transfected lysate(62.70 KDa).Lane 2: Non-transfected lysate.
Staining of nuclear Jurkat lysate using CDC6 ANtibody Cat.-No AP32719PU-N at 0.01 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CDC6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808380)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDC6 (RC206814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDC6.(1:2000)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CDC6 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808381)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CDC6 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808381)(1:150)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-CDC6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808381)(1:150)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CDC6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808381)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CDC6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808381)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CDC6 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808381)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDC6 (RC206814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDC6.(1:500)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDC6 (RC206814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDC6.(1:2000)
Immunohistochemical analysis of CDC6 (pS54) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of CDC6 (pS54) expression in HeLa serum starvation-treated (A); Raw264.7 serum starvation-treated (B); H9C2 serum starvation-treated (C) whole cell lysates.
Predicted band size: 63 kDa . Positive control: K562 and 293T cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: K562 cell lysate Lane2: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes)
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human breast cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using CDC6 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted band size: 63 kDa . Positive control: K562 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes)
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human lung cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using CDC6 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN206814 | CDC6 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN206814BN | CDC6 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN206814LP | CDC6 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN206814RB | CDC6 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
