CDCA3 antibody
Principal name
CDCA3 antibody
Alternative names for CDCA3 antibody
C8, GRCC8, TOME1, TOME-1, Cell division cycle-associated protein 3, Trigger of mitotic entry protein 1, Gene-rich cluster protein C8
Ncbi ID
9606, NP_112589.1, NM_031299, NP_112589, NM_013538, NP_038566.1
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of CDCA3 antibody
- Kit Component:
- - KN302990G1, Cdca3 gRNA vector 1 in pCas-Guide vector
- KN302990G2, Cdca3 gRNA vector 2 in pCas-Guide vector
- KN302990D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Detection limit for recombinant GST tagged CDCA3 is approximately 3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CDCA3 is approximately 10ng/ml as a capture antibody.
Western Blot analysis of CDCA3 expression in transfected 293T cell line (H00083461-T01) by CDCA3 MaxPab polyclonal antibody.Lane 1: CDCA3 transfected lysate(29.48 KDa).Lane 2: Non-transfected lysate.
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of extracts from HepG2 cells, using CDCA3 Antibody Cat.-No AP32284PU-N. The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of CDCA3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CDCA3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of CDCA3 expression in MCF7 (A); Caco2 (B); HepG2 (C) whole cell lysates.
Western blot analysis of extracts from HepG2 cells, using CDCA3 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using CDCA3 antibody.The picture on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315871 | CDCA3 antibody |
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| Rabbit polyclonal anti-CDCA3 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| AP32284PU-N | CDCA3 (C-term) antibody |
||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
50 µl /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300821 | CDCA3 |
||||
| Recombinant protein of human cell division cycle associated 3 (CDCA3) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY410572 | CDCA3 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC200821L2V | Lenti ORF particles, CDCA3 (mGFP-tagged) - Human cell division cycle associated 3 (CDCA3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,020.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203531L2V | Lenti ORF particles, Cdca3 (GFP-tagged) - Mouse cell division cycle associated 3 (Cdca3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR203531L1V | Lenti ORF particles, Cdca3 (Myc-DDK-tagged) - Mouse cell division cycle associated 3 (Cdca3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||