CDK4 antibody
Principal name
CDK4 antibody
Alternative names for CDK4 antibody
PSK-J3, Cell division protein kinase 4, Cyclin-dependent kinase 4
SwissProt ID
P11802 (Human), P30285 (Mouse), P35426 (Rat), P79432 (Pig), Q32KY4 (Bovin), Q91727 (Xenla)
Ncbi ID
NP_000066.1, 9606, NP_000066, 10090, NM_000075 (Gene ID : 1019), NP_000066.1 (Gene ID : 1019), NP_446045.1, NP_034000.1, NM_009870, NM_053593, NM_000075
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))
Background of CDK4 antibody
p34cdk4 is a major catalytic subunit of mammalian D-type cyclins which is expressed by a gene found in human chromosome 12q13. The cdk4 protein is constitutively expressed throughout the cell cycle and it is the assembly and accumulation of functional cdk4/cyclin D complexes which is rate limiting for the progression through the G1 phase of the cell cycle. Once the complex is formed it must be activated by the phosphorylation of threonine 172 on the cdk4 subunit, a reaction mediated by a cdk-activating kinase (CAK). Complex formation and phosphorylation are independently regulated events. The phosphorylation of the cdk4 subunit is subject to regulation of both stimulatory and inhibitory cytokine signals, thereby allowing cdk4 to function as a mid G1 checkpoint. Independent of phosphorylation, cdk4 is also regulated by the binding of p16Ink4/mts1 while in complex with cyclin D.
General readings
Coleman KG, et al. (1997). J. Biol. Chem. 272 (30): 18869–74.Li J, Melvin WS, Tsai MD, Muscarella P (2004). Biochemistry 43 (14): 4394–9.Dai K, Kobayashi R, Beach D (1996). J. Biol. Chem. 271 (36): 22030–4.
Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell. [antibody concentration 10 ug/ml]
Western blot analysis of CDK4 over-expressed 293 cell line, cotransfected with CDK4 Validated Chimera RNAi ( Cat # H00001019-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CDK4 monoclonal antibody (M03) clone 4F11 (Cat # H00001019-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
CDK4 monoclonal antibody (M03), clone 4F11 Western Blot analysis of CDK4 expression in HeLa ( Cat # L013V1 ).
Western Blot analysis of CDK4 expression in transfected 293T cell line by CDK4 monoclonal antibody (M03), clone 4F11.Lane 1: CDK4 transfected lysate(33.7 KDa).Lane 2: Non-transfected lysate.
CDK4 monoclonal antibody (M03), clone 4F11. Western Blot analysis of CDK4 expression in NIH/3T3 ( Cat # L018V1 ).
CDK4 monoclonal antibody (M03), clone 4F11. Western Blot analysis of CDK4 expression in Raw 264.7 ( Cat # L024V1 ).
Detection limit for recombinant GST tagged CDK4 is approximately 1ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell. [antibody concentration 10 ug/ml]
CDK4 monoclonal antibody (M04), clone 2G7 Western Blot analysis of CDK4 expression in HeLa ( Cat # L013V1 ).
CDK4 monoclonal antibody (M04), clone 2G7. Western Blot analysis of CDK4 expression in NIH/3T3 ( Cat # L018V1 ).
CDK4 monoclonal antibody (M04), clone 2G7. Western Blot analysis of CDK4 expression in Raw 264.7 ( Cat # L024V1 ).
Detection limit for recombinant GST tagged CDK4 is approximately 0.1ng/ml as a capture antibody.
CDK4 monoclonal antibody (M05), clone 2A1. Western Blot analysis of CDK4 expression in NIH/3T3 ( Cat # L018V1 ).
CDK4 monoclonal antibody (M05), clone 2A1. Western Blot analysis of CDK4 expression in Raw 264.7 ( Cat # L024V1 ).
CDK4 monoclonal antibody (M05), clone 2A1 Western Blot analysis of CDK4 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged CDK4 is approximately 0.3ng/ml as a capture antibody.
CDK4 monoclonal antibody (M07), clone 8C3. Western Blot analysis of CDK4 expression in NIH/3T3 ( Cat # L018V1 ).
CDK4 monoclonal antibody (M07), clone 8C3. Western Blot analysis of CDK4 expression in Raw 264.7 ( Cat # L024V1 ).
CDK4 monoclonal antibody (M07), clone 8C3 Western Blot analysis of CDK4 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged CDK4 is approximately 0.3ng/ml as a capture antibody.
CDK4 monoclonal antibody (M08), clone 3E7 Western Blot analysis of CDK4 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged CDK4 is approximately 0.3ng/ml as a capture antibody.
Human HepG2; WB Suggested Anti-CDK4 Antibody Titration: 1ug/ml. Positive Control: HepG2 cell lysate; CDK4 antibody - C-terminal region (AVARP03017_T100) in Human HepG2 cells using Western Blot
Human HepG2; WB Suggested Anti-CDK4 Antibody Titration: 1.0ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; CDK4 antibody - C-terminal region (AVARP00001_T100) in Human HepG2 cells using Western Blot
CDK4 monoclonal antibody (M06), clone 6D7 Western Blot analysis of CDK4 expression in HeLa ( Cat # L013V1 ).
CDK4 monoclonal antibody (M06), clone 6D7. Western Blot analysis of CDK4 expression in NIH/3T3 ( Cat # L018V1 ).
Detection limit for recombinant GST tagged CDK4 is approximately 3ng/ml as a capture antibody.
CDK4 MaxPab polyclonal antibody. Western Blot analysis of CDK4 expression in human pancreas.
Western Blot analysis of CDK4 expression in transfected 293T cell line (H00001019-T01) by CDK4 MaxPab polyclonal antibody.Lane 1: CDK4 transfected lysate(33.33 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with CDK4 polyclonal antibody ( Cat # PAB2914 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Western blot analysis of CDK4 polyclonal antibody ( Cat # PAB2914 ) in HL-60 cell lysate. CDK4 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
CDK4 monoclonal antibody (M08), clone 3E7 Western Blot analysis of CDK4 expression in HeLa ( Cat # L013V1 ).
Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell. [antibody concentration 10 ug/ml]
CDK4 monoclonal antibody (M09), clone 8A10 Western Blot analysis of CDK4 expression in HeLa ( Cat # L013V1 ).
CDK4 monoclonal antibody (M09), clone 8A10. Western Blot analysis of CDK4 expression in NIH/3T3 ( Cat # L018V1 ).
CDK4 monoclonal antibody (M09), clone 8A10. Western Blot analysis of CDK4 expression in Raw 264.7 ( Cat # L024V1 ).
Detection limit for recombinant GST tagged CDK4 is approximately 3ng/ml as a capture antibody.
Western Blot analysis of CDK4 expression in transfected 293T cell line by CDK4 monoclonal antibody (M10), clone 1E1.Lane 1: CDK4 transfected lysate(33.7 KDa).Lane 2: Non-transfected lysate.
CDK4 MaxPab polyclonal antibody. Western Blot analysis of CDK4 expression in human pancreas.
Western Blot analysis of CDK4 expression in transfected 293T cell line (H00001019-T01) by CDK4 MaxPab polyclonal antibody.Lane 1: CDK4 transfected lysate(33.33 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Analysis (PLA) of protein-protein interactions between CDK4 and CDKN1B HeLa cells were stained with anti-CDK4 rabbit purified polyclonal 1:1200 and anti-CDKN1B mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of CDK4 expression in transfected 293T cell line (H00001019-T02) by CDK4 MaxPab polyclonal antibody.Lane 1: CDK4 transfected lysate(33.70 KDa).Lane 2: Non-transfected lysate.
Breast: Formalin-Fixed Paraffin-Embedded (FFPE)
Immunoblot analysis of cell lysates.Lane 1 : Romas cell lysate. Lnae 2 : SK-N-MC cell lysate.Lane 3 : MCF-7 cell lysate.Lane 4 : Jurkat cell lysate.Lane 5 : PC-12 cell lysate.Lane 6 : NIH/3T3 cell lysate.
The cell lysate of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CDK4 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Predicted band size: 34kDa. Positive control: Hela and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Hela cell lysate Lane2: 293T cell lysate Lysates: 30 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CDK4 antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification: x200)
Western blot analysis of anti-CDK4 Pab (Cat. #TA324944) in HL-60 cell lysate. CDK4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot analysis of CDK4 (arrow) using rabbit polyclonal CDK4 Antibody (C-term) (Cat. #TA324944). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDK4 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human prostate carcinoma with CDK4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF image of Hela cell stained with CDK4 Antibody (C-term)(Cat#TA324944).Hela cells were incubated with CDK4 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).CDK4 immunoreactivity is localized to Cytoplasm significantly.
CDK4 Antibody (C-term) (Cat. #TA324944) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of extracts of various cell lines, using CDK4 antibody.
Immunohistochemistry of paraffin-embedded human esophageal cancer using CDK4 antibody at dilution of 1:100 (400x lens).
Immunohistochemistry of paraffin-embedded human esophagus using CDK4 antibody at dilution of 1:100 (400x lens).
Immunohistochemistry of paraffin-embedded rat lung using CDK4 antibody at dilution of 1:100 (400x lens).
Immunohistochemistry of paraffin-embedded mouse lung using CDK4 antibody at dilution of 1:100 (400x lens).
Western blot analysis of extracts from Hela cells, using anti-Cdk4, Poly6333.
Rat Cdk4 Antibody (C-term) (Cat. #TA328198) western blot analysis in rat lung tissue lysates (35ug/lane).This demonstrates the rat Cdk4 antibody detected the rat Cdk4 protein (arrow).
WB Suggested Anti-CDK4 Antibody Titration: 1.0ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
Western blot detection of CDK4 in Hela,Lncap and F9 cell lysates using CDK4 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:33KDa.
Western blot detection of CDK4 in Hela,C6 and F9 cell lysates using CDK4 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:34KDa.
Western blot analysis of extracts from Hela cells, using anti-Cdk4, Poly6333.
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA324944 | CDK4 (C-term) antibody |
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| Rabbit polyclonal CDK4 Antibody (C-term) | |||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody! |
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| TA326832 | CDK4 antibody |
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| Rabbit anti-CDK4 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody! |
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| TA327917 | CDK4 antibody |
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| Rabbit Polyclonal anti-Cdk4 Antibody | |||||||
 |
Rabbit | IgG | Poly6333 | Purified | Hu, Ms, Rt | WB |
0.5 ml /
€335.00
|
| OriGene Technologies, Inc. | |||||||
| TA328198 | CDK4 antibody |
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| Rabbit polyclonal anti-R Cdk4 antibody (C-term) | |||||||
 |
Rabbit | Purified | Rt | WB |
0.4 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
| TA346909 | CDK4 antibody |
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| Mouse Monoclonal CDK4 Antibody | |||||||
 |
Mouse | IgG1 | 3F9-B12-C8 | Purified | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AM05224PU-N | CDK4 antibody |
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 |
Mouse | IgG1 | DCS-31.2 | Purified | Hu, Ms, Rt | ICC/IF, IP, WB |
0.1 mg /
€400.00
|
| OriGene Technologies GmbH | |||||||
| AM20604PU-N | CDK4 antibody |
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|
Mouse | IgG2a | IML-4 | Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 mg /
€390.00
|
| OriGene Technologies GmbH | |||||||
| AM50057PU-N | CDK4 antibody |
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 |
Mouse | IgG2a | AT6D10 | Purified | Hu | E, WB |
0.1 ml /
€330.00
|
| OriGene Technologies GmbH | |||||||
| AM50057PU-S | CDK4 antibody |
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|
Mouse | IgG2a | AT6D10 | Purified | Hu | E, WB |
50 µl /
€220.00
|
| OriGene Technologies GmbH | |||||||
| AP05117PU-N | CDK4 antibody |
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|
Rabbit | IgG | Purified | Hu | IP, WB |
0.1 mg /
€400.00
|
|
| OriGene Technologies GmbH | |||||||
| AP08391PU-N | CDK4 (C-term) antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | IP, P, WB |
0.1 ml /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| R1004 | CDK4 antibody |
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Rabbit | Serum | Hu, Ms, Rt | E, IP, WB |
0.1 ml /
€515.00
|
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| OriGene Technologies GmbH | |||||||
| SM1570P | CDK4 antibody |
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|
Mouse | IgG1 | DCS-31.2 | Purified | Hu, Ms, Rt | C, IP, WB |
0.1 mg /
€690.00
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301156 | CDK4 |
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| Recombinant protein of human cyclin-dependent kinase 4 (CDK4) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR204246L2V | Lenti ORF particles, Cdk4 (GFP-tagged) - Mouse cyclin-dependent kinase 4 (Cdk4), 200 uL, >10^7 TU/mL |
|
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200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR204246L1V | Lenti ORF particles, Cdk4 (Myc-DDK-tagged) - Mouse cyclin-dependent kinase 4 (Cdk4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR210857L2V | Lenti ORF particles, Cdk4 (GFP-tagged ORF) - Rat cyclin-dependent kinase 4 (Cdk4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR210857L1V | Lenti ORF particles, Cdk4 (Myc-DDK-tagged ORF) - Rat cyclin-dependent kinase 4 (Cdk4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
