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CDK6 antibody

Principal name

CDK6 antibody

Alternative names for CDK6 antibody

PLSTIRE, Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE

SwissProt ID

Q00534 (Human), Q64261 (Mouse)

Gene ID

1021 (CDK6), 12571 (Cdk6)

Ncbi ID

NP_001138778.1, 9606, NP_001250, NP_001138778, NP_034003.1, NM_001259 (Gene ID : 1021), NP_001250.1 (Gene ID : 1021), NM_001259, NM_001145306, NM_009873

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection))

Background of CDK6 antibody

CDK6 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb.The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb.

General readings

1. Chilosi, M.; Doglioni, C.; Yan, Z.; Lestani, M.; Menestrina, F.; Sorio, C.; Benedetti, A.; Vinante, F.; Pizzolo, G.; Inghirami, G. : Differential expression of cyclin-dependent kinase 6 in cortical thymocytes and T-cell lymphoblastic lymphoma/leukemia. Am. J. Path. 152: 209-217, 1998.
2. Shennan, M. G.; Badin, A.-C.; Walsh, S.; Summers, A.; From, L.; McKenzie, M.; Goldstein, A. M.; Tucker, M. A.; Hogg, D.; Lassam, N. : Lack of germline CDK6 mutations in familial melanoma. Oncogene 19: 1849-1852, 2000.
3 Lien, H.-C.; Lin, C.-W.; Huang, P.-H.; Chang, M.-L.; Hsu, S.-M. : Expression of cyclin-dependent kinase 6 (cdk6) and frequent loss of CD44 in nasal-nasopharyngeal NK/T-cell lymphomas: comparison with CD56-negative peripheral T-cell lymphomas. Lab. Invest. 80: 893-900, 2000.

Immunoperoxidase of monoclonal antibody to CDK6 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]

H00001021-M01

Immunofluorescence of monoclonal antibody to CDK6 on HeLa cell. [antibody concentration 10 ug/ml]

H00001021-M01

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDK2 and CDK6 HeLa cells were stained with anti-CDK2 rabbit purified polyclonal 1:1200 and anti-CDK6 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

H00001021-M01

Western Blot analysis of CDK6 expression in transfected 293T cell line by CDK6 monoclonal antibody (M01), clone 8H4.Lane 1: CDK6 transfected lysate(36.9 KDa).Lane 2: Non-transfected lysate.

H00001021-M01

CDK6 monoclonal antibody (M01), clone 8H4 Western Blot analysis of CDK6 expression in Jurkat ( Cat # L017V1 ).

H00001021-M01

CDK6 monoclonal antibody (M01), clone 8H4. Western Blot analysis of CDK6 expression in PC-12 ( Cat # L012V1 ).

H00001021-M01

Human Jurkat; WB Suggested Anti-CDK6 Antibody Titration: 0.625ug/ml. Positive Control: Jurkat cell lysate. CDK6 is supported by BioGPS gene expression data to be expressed in Jurkat.; CDK6 antibody - C-terminal region (AVARP03005_T100) in Human Jurkat cells using Western Blot

AVARP03005_T100

Western Blot analysis of CDK6 expression in transfected 293T cell line (H00001021-T01) by CDK6 MaxPab polyclonal antibody.Lane 1: CDK6 transfected lysate(35.86 KDa).Lane 2: Non-transfected lysate.

H00001021-B01

Figure 1. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CDK6 (phospho-Tyr24) Antibody AP08040PU.

Figure 1. Immunofluorescence staining of methanol-fixed HeLa cells using CDK6 (phospho-Tyr24) Antibody (#AP08040PU, Red).

Western blot analysis of extracts from 293 cells untreated (lane1) or treated with starvation (lane2) using CDK6 (pTyr24) antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CDK6 Antibody AP08101PU.

Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using CDK6 antibody AP08101PU (Red).

Figure 1. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CDK6 Antibody AP08102PU.

Immunoblot analysis of Hela cell lysate.

MAB0773

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDK6 and CDKN1B HeLa cells were stained with anti-CDK6 rabbit purified polyclonal 1:1200 and anti-CDKN1B mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

H00001021-D01P

Western Blot analysis of CDK6 expression in transfected 293T cell line (H00001021-T02) by CDK6 MaxPab polyclonal antibody.Lane 1: CDK6 transfected lysate(36.90 KDa).Lane 2: Non-transfected lysate.

H00001021-D01P

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDK6 pTyr13 Antibody (#AP09488PU).

Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using CDK6 pTyr13 Antibody (#AP09488PU, Red).

Figure 3. Western blot analysis of extracts from 293 cells untreated or treated with HU using CDK6 pTyr13 Antibody (#AP09488PU).

Western Blot analysis of CDK6 expression in transfected 293T cell line (H00001021-T03) by CDK6 MaxPab polyclonal antibody.Lane 1: CDK6 transfected lysate(35.86 KDa).Lane 2: Non-transfected lysate.

H00001021-D03

Immunoprecipitation of CDK6 transfected lysate using anti-CDK6 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CDK6 MaxPab mouse polyclonal antibody (B01) (H00001021-B01).

H00001021-D03

Figure 1. Western blot analysis of CDK6 expression in HeLa cells (A), Rat-1 cells (B-1) and NIH/3T3 cells (B-2) using CDK6 antibody (BM5021A).

BM5021A

Figure 2. Immunoprecipitation of CDK6 from NIH/3T3 cells with normal Mouse IgG (Lane 1) or BM5021A (Lane 2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with BM5021A.

BM5021A

Figure 1. Immunoprecipitation of CDK6 from Jurkat cells with AM20024AF-N (Lane 1) or Mouse IgG2a (Lane 2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM20024AF-N.

AM20024AF-N

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

AM31156PU-N

Immunofluorescence of monoclonal antibody to CDK6 on HeLa cell. [antibody concentration 10 µg/ml]

AM31156PU-N

Western Blot analysis of CDK6 expression in transfected 293T cell line by CDK6 monoclonal antibody, clone 8H4.

AM31156PU-N

CDK6 monoclonal antibody, clone 8H4 Western Blot analysis of CDK6 expression in Jurkat.

AM31156PU-N

CDK6 monoclonal antibody, clone 8H4. Western Blot analysis of CDK6 expression in PC-12.

AM31156PU-N

TP308560

TP327978

Predicted band size: 37kDa.Positive control: Jurkat cell lysate.Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/300 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 10 seconds)

TA323938

Predicted cell location: Cytoplasm.Positive control: Human colon cancer tissue.Recommended dilution: 1/25-150. The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CDK6 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification: x200)

TA323938

Predicted band size: 37 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/150 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)

TA323939

Predicted band size: 37 kDa . Positive control: HT29 and293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2: HT29 and293 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA323941

Predicted cell location: Cytoplasm; Nucleus. Positive control: Human colon carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human colon carcinoma tissue using CDK6 (Ab-24) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification :x200)

TA323941

Western blot analysis of extracts of various cell lines, using CDK6 antibody.

TA327072

Immunofluorescence analysis of MCF7 cell using CDK6 antibody. Blue: DAPI for nuclear staining.

TA327072

WB Suggested Anti-CDK6 Antibody Titration: 0.625ug/ml; Positive Control: Jurkat cell lysateCDK6 is supported by BioGPS gene expression data to be expressed in Jurkat

TA330350

Western blot analysis of extracts from 293 cells using Phospho-CDK6-Y13 antibody.

TA333180

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using Phospho-CDK6-Y13 antibody.

TA333180

Immunofluorescence staining of methanol- fixed Hela cells using Phospho-CDK6-Y13 antibody.

TA333180

Predicted band size: 37 kDa . Positive control: 293 cells treated with starvation lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: 293 cells untreated with starvation lysate Lane2: 293 cells treated with starvation lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA323940

Predicted cell location: Cytoplasm; Nucleus. Positive control: Human colon carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human colon carcinoma tissue using CDK6 (phospho-Tyr24) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)

TA323940

Predicted cell location: Cytoplasm; Nucleus. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using CDK6 (phospho-Tyr24) antibody at dilution 1/100. (Original magnification: ×200)

TA323940

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
AM31156PU-N	CDK6 antibody
	Mouse	IgG1	8H4	Purified	Hu, Rt	E, ICC/IF, P, WB	50 µg / €475.00
							OriGene Technologies GmbH
+4 additional images	Highly validated antibody!
TA323938	CDK6 antibody
	Anti-CDK6 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA323939	CDK6 antibody
	Anti-CDK6 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA323940	CDK6 antibody
	Anti-CDK6 (phospho-Tyr24) Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms	ICC/IF, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+2 additional images
TA323941	CDK6 antibody
	Anti-CDK6 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms	P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA327072	CDK6 antibody
	Rabbit anti-CDK6 Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt		0.1 mg / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA333180	CDK6 antibody
	Phospho-CDK6-Y13 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms		0.1 mg / €335.00
							OriGene Technologies, Inc.
+2 additional images
AM05226PU-N	CDK6 antibody
	Mouse	IgG1	DCS-83.1	Purified	Hu	C, IP, WB	0.1 mg / €400.00
							OriGene Technologies GmbH
AM20024AF-N	CDK6 antibody
	Mouse	IgG2a	DCS-130	Azide Free	Hu	ICC/IF, IP, P	0.1 mg / €320.00
							OriGene Technologies GmbH
AM20605PU-N	CDK6 antibody
	Mouse	IgG1	IML-6	Purified	Hu, Ms, Rt	ICC/IF, WB	0.1 mg / €390.00
							OriGene Technologies GmbH
AP08040PU-N	CDK6 pTyr24 antibody
	Rabbit			Aff - Purified	Hu, Ms	ICC/IF, P, WB	0.1 mg / €300.00
							OriGene Technologies GmbH
+2 additional images
AP08040PU-S	CDK6 pTyr24 antibody
	Rabbit			Aff - Purified	Hu, Ms	ICC/IF, P, WB	50 µg / €230.00
							OriGene Technologies GmbH
+2 additional images
AP08101PU-N	CDK6 antibody
	Rabbit			Aff - Purified	Hu, Ms	ICC/IF, P	0.1 mg / €300.00
							OriGene Technologies GmbH
+1 additional image
AP08101PU-S	CDK6 antibody
	Rabbit			Aff - Purified	Hu, Ms	ICC/IF, P	50 µg / €230.00
							OriGene Technologies GmbH
+1 additional image
AP08102PU-N	CDK6 antibody
	Rabbit			Aff - Purified	Hu, Ms	P	0.1 mg / €300.00
							OriGene Technologies GmbH
AP08102PU-S	CDK6 antibody
	Rabbit			Aff - Purified	Hu, Ms	P	50 µg / €230.00
							OriGene Technologies GmbH
AP09488PU-N	CDK6 pTyr13 antibody
	Rabbit			Aff - Purified	Hu, Ms	ICC/IF, P, WB	0.1 mg / €300.00
							OriGene Technologies GmbH
+2 additional images
AP09488PU-S	CDK6 pTyr13 antibody
	Rabbit			Aff - Purified	Hu, Ms	ICC/IF, P, WB	50 µg / €230.00
							OriGene Technologies GmbH
+2 additional images
BM5021A	CDK6 antibody
	Mouse	IgG1	DCS-83	Azide Free	Hu, Ms, Rt	IP, WB	0.1 mg / €350.00
							OriGene Technologies GmbH
+1 additional image
SM1568P	CDK6 antibody
	Mouse	IgG1	DCS-83.1	Purified	Hu	C, IP, WB	0.1 mg / €610.00
							OriGene Technologies GmbH