CDK8 antibody
Principal name
CDK8 antibody
Alternative names for CDK8 antibody
Protein kinase K35, Cell division protein kinase 8, Cyclin-dependent kinase 8, Mediator of RNA polymerase II transcription subunit CDK8, Mediator complex subunit CDK8
Ncbi ID
9606, NP_001251, NP_001251.1, NM_001260 (Gene ID : 1024), NP_001251.1 (Gene ID : 1024), NM_001260, NP_705827.2, NM_153599
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of CDK8 antibody
- Kit Component:
- - KN303050G1, Cdk8 gRNA vector 1 in pCas-Guide vector
- KN303050G2, Cdk8 gRNA vector 2 in pCas-Guide vector
- KN303050D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Akoulitchev,S., et al., (2000) Nature 407 (6800), 102-106
CDK8 monoclonal antibody (M01), clone 6H5. Western Blot analysis of CDK8 expression in NIH/3T3 ( Cat # L018V1 ).
CDK8 monoclonal antibody (M01), clone 6H5. Western Blot analysis of CDK8 expression in Jurkat ( Cat # L017V1 ).
CDK8 monoclonal antibody (M01), clone 6H5. Western Blot analysis of CDK8 expression in Raw 264.7 ( Cat # L024V1 ).
CDK8 monoclonal antibody (M01), clone 6H5. Western Blot analysis of CDK8 expression in PC-12 ( Cat # L012V1 ).
CDK8 monoclonal antibody (M01), clone 6H5 Western Blot analysis of CDK8 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged CDK8 is approximately 0.03ng/ml as a capture antibody.
CDK8 monoclonal antibody (M02), clone 6E5. Western Blot analysis of CDK8 expression in NIH/3T3 ( Cat # L018V1 ).
CDK8 monoclonal antibody (M02), clone 6E5. Western Blot analysis of CDK8 expression in Jurkat ( Cat # L017V1 ).
CDK8 monoclonal antibody (M02), clone 6E5. Western Blot analysis of CDK8 expression in Raw 264.7 ( Cat # L024V1 ).
CDK8 monoclonal antibody (M02), clone 6E5. Western Blot analysis of CDK8 expression in PC-12 ( Cat # L012V1 ).
CDK8 monoclonal antibody (M02), clone 6E5 Western Blot analysis of CDK8 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged CDK8 is approximately 0.03ng/ml as a capture antibody.
CDK8 monoclonal antibody (M03), clone 5H4. Western Blot analysis of CDK8 expression in NIH/3T3 ( Cat # L018V1 ).
CDK8 monoclonal antibody (M03), clone 5H4. Western Blot analysis of CDK8 expression in K-562 ( Cat # L009V1 ).
CDK8 monoclonal antibody (M03), clone 5H4. Western Blot analysis of CDK8 expression in PC-12 ( Cat # L012V1 ).
CDK8 monoclonal antibody (M03), clone 5H4. Western Blot analysis of CDK8 expression in Jurkat ( Cat # L017V1 ).
CDK8 monoclonal antibody (M03), clone 5H4. Western Blot analysis of CDK8 expression in Raw 264.7 ( Cat # L024V1 ).
CDK8 monoclonal antibody (M03), clone 5H4 Western Blot analysis of CDK8 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged CDK8 is approximately 0.03ng/ml as a capture antibody.
CDK8 monoclonal antibody (M04), clone 2E6. Western Blot analysis of CDK8 expression in NIH/3T3 ( Cat # L018V1 ).
CDK8 monoclonal antibody (M04), clone 2E6. Western Blot analysis of CDK8 expression in PC-12 ( Cat # L012V1 ).
CDK8 monoclonal antibody (M04), clone 2E6. Western Blot analysis of CDK8 expression in Raw 264.7 ( Cat # L024V1 ).
CDK8 monoclonal antibody (M04), clone 2E6 Western Blot analysis of CDK8 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged CDK8 is approximately 0.1ng/ml as a capture antibody.
Human HepG2; WB Suggested Anti-CDK8 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate; CDK8 antibody - C-terminal region (AVARP03050_T100) in Human HepG2 cells using Western Blot
Western blot (WB) analysis of CDK8 antibody (Cat.-No.: AP06697PU-N) at 1/500 dilution
Lane1:HEK293T whole cell lysate
Lane2:sp2/0 whole cell lysate
Lane3:H9C2 whole cell lysate
Lane1:HEK293T whole cell lysate
Lane2:sp2/0 whole cell lysate
Lane3:H9C2 whole cell lysate
Western blot analysis of CDK8 polyclonal antibody ( Cat # PAB2918 ) in HL-60 cell lysate. CDK8 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot analysis of CDK8 antibody (N-term) in Jurkat cell line lysates (35ug/lane). CDK8 (arrow) was detected using the purified Pab
Immunohistochemical analysis of CDK8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of CDK8 expression in HepG2 (A); A549 (B); Raw264.7 (C) whole cell lysates.
WB Suggested Anti-CDK8 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:12500; Positive Control: HepG2 cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP06697PU-N | CDK8 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms | P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| AP14153PU-N | CDK8 (N-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN212592 | CDK8 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN212592BN | CDK8 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN212592LP | CDK8 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN212592RB | CDK8 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP312592 | CDK8 |
||||
| Recombinant protein of human cyclin-dependent kinase 8 (CDK8) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY400507 | CDK8 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR218219L2V | Lenti ORF particles, Cdk8 (GFP-tagged) - Mouse cyclin-dependent kinase 8 (Cdk8), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,140.00
|
|
| OriGene Technologies, Inc. | ||
| MR218219L1V | Lenti ORF particles, Cdk8 (Myc-DDK-tagged) - Mouse cyclin-dependent kinase 8 (Cdk8), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,020.00
|
|
| OriGene Technologies, Inc. | ||