CDKL1 antibody
Principal name
CDKL1 antibody
Alternative names for CDKL1 antibody
KKIALRE, Cyclin-dependent kinase-like 1, Serine/threonine-protein kinase KKIALRE, Protein kinase p42 KKIALRE
Ncbi ID
9606, NP_004187.2, NP_004187, NM_004196, NP_899117.1, NM_183294, NM_001025121
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of CDKL1 antibody
- Kit Component:
- - KN303053G1, Cdkl1 gRNA vector 1 in pCas-Guide vector
- KN303053G2, Cdkl1 gRNA vector 2 in pCas-Guide vector
- KN303053D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunoperoxidase of monoclonal antibody to CDKL1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 5 ug/ml]
CDKL1 monoclonal antibody (M01), clone 5B11. Western Blot analysis of CDKL1 expression in human stomach.
Detection limit for recombinant GST tagged CDKL1 is approximately 0.03ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to CDKL1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 5 ug/ml]
Detection limit for recombinant GST tagged CDKL1 is approximately 0.03ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to CDKL1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 5 ug/ml]
Detection limit for recombinant GST tagged CDKL1 is approximately 0.1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CDKL1 is approximately 0.1ng/ml as a capture antibody.
Western blot analysis of KKIALRE antibody (Cat.-No. AP06708PU-N) in extracts from COLO205 cells.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
The anti-CDKL1 Pab is used in Western blot to detect CDKL1 in mouse kidney tissue lysate.
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection limit for recombinant GST tagged CDKL1 is approximately 0.1 ng/ml as a capture antibody.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CDKL1 mouse monoclonal antibody. (TA805781; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CDKL1 mouse monoclonal antibody. (TA805781; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKL1 (RC211223, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKL1.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CDKL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805785)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKL1 (RC211223, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKL1 (RC211223, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKL1 (RC211223, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKL1.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CDKL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805953)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKL1 (RC211223, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKL1 (RC211223, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKL1.
Immunohistochemical analysis of CDKL1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of CDKL1 expression in HEK293T (A); NIH3T3 (B) whole cell lysates.
Western blot analysis of extracts from COLO205 cells, using CDKL1 antibody.The lane on the right is treated with the synthesized peptide.
Host: Rabbit; Target Name: CDKL1; Sample Tissue: U937 Whole Cell lysates; Antibody Dilution: 1.0ug/ml
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314812 | CDKL1 antibody |
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| Rabbit polyclonal anti-CDKL1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA805781 | CDKL1 antibody |
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| CDKL1 mouse monoclonal antibody, clone OTI1D9 (formerly 1D9) | |||||||
 |
Mouse | IgG1 | OTI1D9 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|
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| TA805785 | CDKL1 antibody |
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| CDKL1 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4) | |||||||
 |
Mouse | IgG2b | OTI1H4 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA805786 | CDKL1 antibody |
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| CDKL1 mouse monoclonal antibody, clone OTI3E8 (formerly 3E8) | |||||||
 |
Mouse | IgG1 | OTI3E8 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA805952 | CDKL1 antibody |
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| CDKL1 mouse monoclonal antibody, clone OTI5B10 (formerly 5B10) | |||||||
 |
Mouse | IgG1 | OTI5B10 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA805953 | CDKL1 antibody |
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| CDKL1 mouse monoclonal antibody, clone OTI7F10 (formerly 7F10) | |||||||
 |
Mouse | IgG1 | OTI7F10 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA806106 | CDKL1 antibody |
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| CDKL1 mouse monoclonal antibody, clone OTI7B7 (formerly 7B7) | |||||||
 |
Mouse | IgG2b | OTI7B7 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AM20963PU-N | CDKL1 antibody |
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 |
Mouse | IgG2a | 2H6 | Hu | E, P, WB |
50 µg /
€475.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AP06708PU-N | CDKL1 antibody |
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 |
Rabbit | Aff - Purified | Hu, Rt | P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| AP14156PU-N | CDKL1 (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY418154 | CDKL1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR213588L2V | Lenti ORF particles, Cdkl1 (GFP-tagged) - Mouse cyclin-dependent kinase-like 1 (CDC2-related kinase) (Cdkl1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR213588L1V | Lenti ORF particles, Cdkl1 (Myc-DDK-tagged) - Mouse cyclin-dependent kinase-like 1 (CDC2-related kinase) (Cdkl1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR201311L2V | Lenti ORF particles, Cdkl1 (GFP-tagged ORF) - Rat cyclin-dependent kinase-like 1 (CDC2-related kinase) (Cdkl1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR201311L1V | Lenti ORF particles, Cdkl1 (Myc-DDK-tagged ORF) - Rat cyclin-dependent kinase-like 1 (CDC2-related kinase) (Cdkl1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||