CDYL2 antibody
Principal name
CDYL2 antibody
Alternative names for CDYL2 antibody
Chromodomain Y-like protein 2, CDY-like 2
SwissProt ID
Q8N8U2 (Human)
Gene ID
124359 (CDYL2)
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of CDYL2 antibody
CDYL2 contains 1 chromo domain. The exact function of CDYL2 remains unknown.
General readings
Dorus,S., (2003) Hum. Mol. Genet. 12 (14), 1643-1650
Immunofluorescence of purified MaxPab antibody to CDYL2 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of CDYL2 expression in transfected 293T cell line (H00124359-T01) by CDYL2 MaxPab polyclonal antibody.Lane 1: CDYL2 transfected lysate(55.66 KDa).Lane 2: Non-transfected lysate.
Human 721_B; WB Suggested Anti-CDYL2 Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysate; CDYL2 antibody - N-terminal region (ARP49048_P050) in Human 721_B cells using Western Blot
Immunohistochemical analysis of CDYL2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CDYL2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of CDYL2 expression in A431 (A); HepG2 (B); HeLa (C) whole cell lysates.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using CDYL2 antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-CDYL2 Antibody Titration: 0.2-1 ug/ml; Positive Control: 721_B cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA316072 | CDYL2 antibody |
||||||
| Rabbit polyclonal anti-CDYLy2 Antibod | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY407592 | CDYL2 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |