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CEBPB antibody

Principal name

CEBPB antibody

Alternative names for CEBPB antibody

C/EBP beta, TCF5, LAP, NF-IL6, CCAAT/enhancer-binding protein beta, Liver activator protein, Nuclear factor NF-IL6, Transcription factor 5

SwissProt ID

O02755 (Bovin), P17676 (Human), P21272 (Rat), P28033 (Mouse), Q05826 (Chick)

Gene ID

1051 (CEBPB), 12608 (Cebpb)

Ncbi ID

NP_005185.2, NP_005185, 9606, NP_034013.1, AAH21931.1, NM_005194

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Por (Porcine), Rt (Rat), Can (Canine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), ChIP assay (ChIP)

Background of CEBPB antibody

C/EBP β (also known as CCAAT/enhancer-binding protein beta, interleukin 6-dependent DNA-binding protein, and nuclear factor of interleukin 6 [NF-IL6]) is a member of the basic leucine zipper C/EBP transcription factor family. C/EBP has three isoforms that include C/EBPβ, LAP, and LIP (35 kD, 32 kD, and 20 kD, respectively). This protein is localized in the nucleus and functions as a transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I gene expression. It is upregulated by growth hormones and has been shown to interact with CEBP-α, CEBP-δ, CEBP-γ, CEBP-ε, and CEBP-ζ. The C/EBP β protein can be modified by phosphorylation and ubiquitination.
Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display different function in gene regulation. From a neurological standpoint, C/EBPs have been implicated in the mediation of nerve growth factor, altering synaptic plasticity, and mediation of inflammatory response in AD patients.
Structure: C/EBP transcription factor family, basic leucine zipper, dimer. Isoforms C/EBPβ, LAP, LIP, approximately 35 kD, 32 kD, 20 kD, respectively.
Distribution: Nuclear.
Function: Transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I genes.
Regulation: Growth hormoneModification:Phosphorylation, Ubiquitination.
Interaction: CEBP-α, CEBP-β, CEBP-δ, CEBP-γ, CEBP-ε, CEBP-ζ

General readings

1. Mir RA & Chauhan SS Down Regulation of a Matrix Degrading Cysteine Protease Cathepsin L, by Acetaldehyde: Role of C/EBPa. PLoS One 6:e20768 (2011). WB ; Human .
2. Younce CW et al. MCP-1 (monocyte chemotactic protein-1)-induced protein, a recently identified zinc finger protein, induces adipogenesis in 3T3-L1 pre-adipocytes without peroxisome proliferator-activated receptor gamma. J Biol Chem 284:27620-8 (2009). WB ; Mouse .

Immunohistochemistry: SM6009 CEBPB antibody staining of Formalin-Fixed, Paraffin-Embedded Human Tonsil at 10 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.

Immunohistochemistry: SM6009 CEBPB antibody staining of Formalin-Fixed, Paraffin-Embedded Human Skin at 10 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.

Western blot analysis: NIH 3T3 cell lysates (30 μg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with SM6009 CEBPB antibody (1/1,000). Protein was visualized using a Goat anti-Mouse secondary antibody conjugated to HRP and an ECL detection system.

Human HepG2; WB Suggested Anti-CEBPB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; CEBPB antibody - C-terminal region (ARP31259_P050) in Human HepG2 cells using Western Blot

ARP31259_P050

Immunohistochemistry (IHC) analyzes of C7EBP-beta antibody (Cat.-No.: AP06034PU-N) in paraffin-embedded human lung carcinoma tissue.

AP06034PU-N

Western blot (WB) analysis of C/EBP-beta antibody (Cat.-No.: AP06034PU-N) at 1/500 dilution
Lane1: Hela cell lysate
Lane2: Mouse brain tissue lysate
Lane3: Rat kidney tissue lysate

AP06034PU-N

Western blot (WB) analysis of C/EBP-β antibody (Cat.-No.: AP06676PU-N) at 1/500 dilution
Lane1:HEK293T whole cell lysate
Lane2:NIH-3T3 whole cell lysate
Lane3:H9C2 whole cell lysate

AP06676PU-N

AM06039PU-N CEBPB Antibody staining of Formalin-Fixed Paraffin-Embedded Human Kidney at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AM06039PU-N

Western blot analysis of NIH/3T3 cell lysates ( 30 µg ) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with CEBPB monoclonal antibody , clone 47A1 ( 1 : 1000 ) ( Cat # MAB1069 ) . Protein was visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.

MAB1069

Western Blot analysis of CEBPB expression in transfected 293T cell line (H00001051-T01) by CEBPB MaxPab polyclonal antibody.Lane 1: CEBPB transfected lysate(37.95 KDa).Lane 2: Non-transfected lysate.

H00001051-B01P

Western blot analysis of CEBPB (arrow) using rabbit polyclonal CEBPB Antibody (C-term) (AP17939PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CEBPB gene (Lane 2) .

AP17939PU-N

Human MCF7; Host:Rabbit. Target Name:CEBPB. Sample Tissue:Human MCF7. Antibody Dilution: 1.0ug/ml. CEBPB is supported by BioGPS gene expression data to be expressed in MCF7.; CEBPB antibody - N-terminal region (ARP57940_P050) in Human MCF7 cells using Western Blot

ARP57940_P050

Human HepG2; WB Suggested Anti-CEBPB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; CEBPB antibody - N-terminal region (ARP57940_P050) in Human HepG2 cells using Western Blot

ARP57940_P050

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using C/EBP-beta (Phospho-Thr235/188) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HepG2 cells, using C/EBP-beta (Phospho-Thr235/188) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HepG2 cells treated with EGF 200ng/ml 30', using C/EBP-beta (Phospho-Thr235/188) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using C/EBP-beta (Ab-235/188) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from NIH-3T3 cells, using C/EBP-beta (Ab-235/188) Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from Jurkat cells, using CEBPB Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis using CEBPB mAb against human CEBPB (AA: 161-338) recombinant protein. (Expected MW is 44.5 kDa)

AM06669SU-N

Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)

AM06669SU-N

Flow cytometric analysis of MCF-7 cells using CEBPB mouse mAb (blue) and negative control (red).

AM06669SU-N

AP32952PU-N (1 µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AP32952PU-N

AP32952PU-N (1µg/ml) staining of Human Skin (A) and Pig Lung (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AP32952PU-N

Nuclear extracts from P388D1 (IL-1) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-C/EBP ß (clone A16). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system. As shown, this antibody specifically recognizes the LAP isoform of C/EBP ß.

P388D1 (IL-1) cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-C/EBP ß Antibody (Clone 1H7). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.

Immunohistochemical analysis of C/EBP beta staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of C/EBP beta expression in HeLa (A); mouse brain (B); rat kidney (C) whole cell lysates.

Western blot analysis of C/EBP beta expression in HeLa (A); NIH3T3 (B); Jurkat (C) whole cell lysates.

Immunohistochemical analysis of C/EBP beta (pT235) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of C/EBP beta (pT235) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of C/EBP beta (pT235) expression in HEK293T EGF-treated (A); COLO205 EGF-treated (B) whole cell lysates.

TP305882

Western blot analysis of extracts from Jurkat cells, using CEBPB antibody.The lane on the right is treated with the synthesized peptide.

TA312910

Western blot analysis of extracts from NIH/3T3 cells, using C/EBP-ÃŸ (Ab-235/188) antibody (#TA312911).The lane on the right is treated with the synthesized peptide.

TA312911

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using C/EBP-ÃŸ (Ab-235/188) antibody (#TA312911).The picture on the right is treated with the synthesized peptide.

TA312911

Predicted band size: 36 kDa . Positive control: Rat brain, Mouse brain tissue and 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2,3: Rat brain, Mouse brain tissue and 293 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA322657

Western blot analysis of C/EBP-beta expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325357

WB Suggested Anti-CEBPB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate

TA344533

Host:Rabbit; Target Name:CEBPB; Sample Tissue:Human MCF7; Antibody Dilution: 1.0 ug/mlCEBPB is supported by BioGPS gene expression data to be expressed in MCF7

TA344533

Western blot analysis on HepG2 cell lysate using CEBPB Antibody

TA347599

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (CEBPB Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: ×200)

TA351070

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (CEBPB Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: ×200)

TA351070

Western blot analysis of C/EBP- beta phosphorylation expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325358

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
SM6009	CEBPB antibody
	Mouse	IgG1	47A1	Purified	Hu, Ms	E, ICC/IF, P, WB	0.1 ml / €330.00
							OriGene Technologies GmbH
+3 additional images	Highly validated antibody!
SM6009S	CEBPB antibody
	Mouse	IgG1	47A1	Purified	Hu, Ms	E, ICC/IF, P, WB	50 µl / €220.00
							OriGene Technologies GmbH
+3 additional images	Highly validated antibody!
AP32952PU-N	CEBPB (68-81) antibody
	Goat			Aff - Purified	Bov, Can, Hu, Ms, Por, Rt	E, WB	0.1 mg / €380.00
							OriGene Technologies GmbH
+1 additional image	OriGene recommends this product for Western blotting.
TA312910	CEBPB antibody
	Rabbit polyclonal anti-CEBPB antibody
	Rabbit	IgG		Purified	Hu, Ms	E, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA312911	CEBPB antibody
	Rabbit polyclonal C/EBP-beta (Ab-235/188) antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA322657	CEBPB antibody
	Anti-CEBPB Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA325357	CEBPB antibody
	Rabbit Polyclonal C/EBP-beta Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA325358	CEBPB antibody
	Rabbit Polyclonal C/EBP- beta (Thr235/188) Antibody (Phospho-specific)
	Rabbit	IgG		Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA347599	CEBPB antibody
	Rabbit Polyclonal Anti-CEBPB Antibody
	Rabbit	IgG		Purified	Hu, Ms	WB	0.1 mg / €335.00
							OriGene Technologies, Inc.
TA351070	CEBPB antibody
	Rabbit Polyclonal Anti-CEBPB Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, P	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
AM06039PU-N	CEBPB (39-78) antibody
	Mouse	IgG	21A1	Azide Free	Hu	IP, P, WB	50 µl / €425.00
							OriGene Technologies GmbH
AM06669SU-N	CEBPB antibody
	Mouse	IgG1	3H9	Ascites	Hu	E, WB	0.1 ml / €410.00
							OriGene Technologies GmbH
+2 additional images
AP06034PU-N	CEBPB antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
+1 additional image
AP06676PU-N	CEBPB antibody
	Rabbit			Aff - Purified	Hu, Ms	P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
AP17939PU-N	CEBPB (C-term) antibody
	Rabbit				Hu	WB	0.4 ml / €415.00
							OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP305882	CEBPB
	Recombinant protein of human CCAAT/enhancer binding protein (C/EBP), beta (CEBPB)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY417451	CEBPB overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

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