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Centractin alpha antibody

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Principal name

Centractin alpha antibody

Alternative names for Centractin alpha antibody

ACTR1A, CTRN1, ARP1, Centrosome-associated actin homolog, Actin-RPV

SwissProt ID

P61163 (Human), P61164 (Mouse), P85515 (Rat)

Gene ID

10121 (ACTR1A), 54130 (Actr1a), 294010

Ncbi ID

9606, NP_005727, NP_005727.1, NP_058556.1, NM_016860, NM_001106364, NM_005736

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Por (Porcine), Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of Centractin alpha antibody

ACTR1A is a 42.6 kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 8-13 copies per dynactin molecule, and is the most abundant molecule in the dynactin complex. It is an actin-related protein, and is approximately 60% identical at the amino acid level to conventional actin.This gene encodes a 42.6 kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 8-13 copies per dynactin molecule, and is the most abundant molecule in the dynactin complex. It is an actin-related protein, and is approximately 60% identical at the amino acid level to conventional actin. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Lees-Miller JP, Helfman DM, Schroer TA.
A vertebrate actin-related protein is a component of a multisubunit complex involved in microtubule-based vesicle motility.
Nature. 1992 Sep 17;359(6392):244-6.
PMID: 1528266

H00010121-A01 - Centractin alpha
ACTR1A polyclonal antibody (A01), Lot # 060125JC01. Western Blot analysis of ACTR1A expression in human ovary cancer.
PAB6617 - Centractin alpha
ACTR1A polyclonal antibody ( Cat # PAB6617 ) staining ( 1 µg/ml ) of human brain lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
H00010121-M08 - Centractin alpha
Detection limit for recombinant GST tagged ACTR1A is approximately 1ng/ml as a capture antibody.
H00010121-B01 - Centractin alpha
ACTR1A MaxPab polyclonal antibody. Western Blot analysis of ACTR1A expression in human liver.
H00010121-B01 - Centractin alpha
Western Blot analysis of ACTR1A expression in transfected 293T cell line (H00010121-T01) by ACTR1A MaxPab polyclonal antibody.Lane 1: ACTR1A transfected lysate(41.36 KDa).Lane 2: Non-transfected lysate.
ARP58414_P050 - Centractin alpha
Human MCF-7; WB Suggested Anti-ACTR1A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; ACTR1A antibody - middle region (ARP58414_P050) in Human MCF-7 cells using Western Blot
AP50073PU-N - Centractin alpha
ACTR1A Antibody (Center) (Cat. #AP50073PU-N) western blot analysis in mouse cerebellum tissue lysates (35µg/lane).This demonstrates the ACTR1A antibody detected the ACTR1A protein (arrow).
TP300738 - Centractin alpha
TA302419 - Centractin alpha
TA302419 staining (2µg/ml) of human kidney lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA321723 - Centractin alpha
Predicted band size: 43 kDa . Positive control: MCF7 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)
TA321724 - Centractin alpha
Predicted cell location: Cytoplasm. Positive control: Human colon and thyroid cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using ACTR1A antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification:x 200)
TA321724 - Centractin alpha
Predicted cell location: Cytoplasm. Positive control: Human colon and thyroid cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using ACTR1A antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification:x200)
TA344351 - Centractin alpha
WB Suggested Anti-ACTR1A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: MCF7 cell lysate

9 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Centractin alpha (C-term) antibody
  Goat Polyclonal Antibody against ARP1 homolog A  
TA302419 staining (2µg/ml) of human kidney lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Hu, Ms, Por, Rt WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

Centractin alpha antibody
  Anti-ACTR1A Rabbit Polyclonal Antibody  
Predicted band size: 43 kDa . Positive control: MCF7 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

Centractin alpha antibody
  Anti-ACTR1A Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human colon and thyroid cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using ACTR1A antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

Centractin alpha (Center) antibody
ACTR1A Antibody (Center) (Cat. #AP50073PU-N) western blot analysis in mouse cerebellum tissue lysates (35µg/lane).This demonstrates the ACTR1A antibody detected the ACTR1A protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Centractin alpha
  Recombinant protein of human ARP1 actin-related protein 1 homolog A, centractin alpha (yeast) (ACTR1A)  
Centractin alpha Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Actr1a (GFP-tagged) - Mouse ARP1 actin-related protein 1 homolog A, centractin alpha (yeast) (Actr1a), 200 uL, >10^7 TU/mL
Lenti ORF particles, Actr1a (GFP-tagged) - Mouse ARP1 actin-related protein 1 homolog A, centractin alpha (yeast) (Actr1a), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Actr1a (Myc-DDK-tagged) - Mouse ARP1 actin-related protein 1 homolog A, centractin alpha (yeast) (Actr1a), 200 uL, >10^7 TU/mL
Lenti ORF particles, Actr1a (Myc-DDK-tagged) - Mouse ARP1 actin-related protein 1 homolog A, centractin alpha (yeast) (Actr1a), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Actr1a (GFP-tagged ORF) - Rat ARP1 actin-related protein 1 homolog A, centractin alpha (yeast) (Actr1a), 200 uL, >10^7 TU/mL
Lenti ORF particles, Actr1a (GFP-tagged ORF) - Rat ARP1 actin-related protein 1 homolog A, centractin alpha (yeast) (Actr1a), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Actr1a (Myc-DDK-tagged ORF) - Rat ARP1 actin-related protein 1 homolog A, centractin alpha (yeast) (Actr1a), 200 uL, >10^7 TU/mL
Lenti ORF particles, Actr1a (Myc-DDK-tagged ORF) - Rat ARP1 actin-related protein 1 homolog A, centractin alpha (yeast) (Actr1a), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

9 Item(s)

per page

Available Filters

Product Type
  1. Mouse Lenti ORF Particle (2)
  2. Primary Antibody (4)
  3. Protein | Growth Factor (1)
  4. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (4)
Target
  1. Centractin alpha (5)
Property
  1. C-term (1)
  2. Center (1)
Reactivity
  1. Human (Hu) (4)
  2. Mouse (Ms) (4)
  3. Rat (Rt) (3)
  4. Porcine (Por) (1)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Enzyme Immunoassay (E) (2)
  3. Paraffin Sections (P) (1)
Presentation
  1. Aff - Purified (2)
  2. Purified (2)
Host
  1. Rabbit (3)
  2. Goat (1)
Images
  1. 1 image (5)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. IHC (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (9)
Service
  • OriGene Technologies GmbH
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  • Germany
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