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CGK2 antibody

Principal name

CGK2 antibody

Alternative names for CGK2 antibody

PRKG2, PRKGR2, cGKII, cGMP-dependent protein kinase 2, Type II cGMP-dependent protein kinase

SwissProt ID

Q13237 (Human), Q61410 (Mouse), Q64595 (Rat)

Gene ID

5593 (PRKG2), 25523 (Prkg2)

Ncbi ID

9606, NP_006250, NP_006250.1, NM_006259, NM_013012

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of CGK2 antibody

cGKII is thought to play a key role in a diverse set of physiological pathway. cGKII may mediate intestinal secretion of water and electrolytes induced by the E. coli toxin STa and the intestinal peptide guanylin. Edentification of the pathway that mediates intestinal fluid secretion by E. coli STa has potential medical implications because STa causes traveler's diarrhea and about 50% of infant mortality in developing countries. Transfection experiments in human cells disclose that cGKII phosphorylates SOX9 and attenuates SOX9 function by inhibiting its nuclear entry. Impaired differentiation of cultured KMI chondrocytes can be restored by silencing Sox9 by RNA interference. cGKII is postulated to be a molecular switch that couples the cessation of proliferation and the start of hypertrophic chondrocyte differentiation through attenuating SOX9 function.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKG2 polyclonal antibody ( Cat # PAB3193 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.

PAB3193

(LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. (RIGHT)Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with cGKII antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

AP14907PU-N

Western blot analysis of cGKII (arrow) using cGKII Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKG2 gene (Lane 2).

AP14907PU-N

Western blot (WB) analysis of cGKii antibody (Cat.-No.: AP2483PU-N) in extracts from hela cells.

AP20532PU-N

Western blot analysis of extracts from 293 cells, treated with anisomycin 25ug/ml 30', using PKG2 (Ab-126) Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HuvEc cells, using CGK 2 Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of cGK 2 expression in HeLa (A); Hs67 (B) whole cell lysates.

TP315879

Western blot analysis of extracts from HUVEC cells, using CGK 2 antibody.The lane on the right is treated with the synthesized peptide.

TA314390

Western blot analysis of extracts from 293 cells, treated with anisomycin (25ug/ml, 30mins), using PKG2 (Ab-126) antibody.The lane on the right is treated with the synthesized peptide.

TA314391

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA314390	CGK2 antibody
	Rabbit polyclonal anti-CGK 2 antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
TA314391	CGK2 antibody
	Rabbit polyclonal PKG2 (Ab-126) antibody
	Rabbit	IgG	Purified	Hu, Ms	E, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
AP14907PU-N	CGK2 (C-term) antibody
	Rabbit	Ig	Purified	Hu	P, WB	0.4 ml / €415.00
						OriGene Technologies GmbH
+1 additional image
AP20532PU-N	CGK2 antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	WB	0.1 mg / €370.00
						OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP315879	CGK2
	Recombinant protein of human protein kinase, cGMP-dependent, type II (PRKG2)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY401884	PRKG2 overexpression lysate
		0.1 mg / €495.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR206197L2V	Lenti ORF particles, Prkg2 (GFP-tagged ORF) - Rat protein kinase, cGMP-dependent, type II (Prkg2), 200 uL, >10^7 TU/mL
		200 µl / €1,550.00
		OriGene Technologies, Inc.

RR206197L1V	Lenti ORF particles, Prkg2 (Myc-DDK-tagged ORF) - Rat protein kinase, cGMP-dependent, type II (Prkg2), 200 uL, >10^7 TU/mL
		200 µl / €1,400.00
		OriGene Technologies, Inc.

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