CHD4 antibody
Principal name
CHD4 antibody
Alternative names for CHD4 antibody
Mi2-beta, Mi-2 beta, CHD-4, Chromodomain-helicase-DNA-binding protein 4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of CHD4 antibody
- Kit Component:
- - KN303248G1, Chd4 gRNA vector 1 in pCas-Guide vector
- KN303248G2, Chd4 gRNA vector 2 in pCas-Guide vector
- KN303248D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunoperoxidase of monoclonal antibody to CHD4 on formalin-fixed paraffin-embedded human transitional cell carcinoma. [antibody concentration 3ug/ml]
Western Blot analysis of CHD4 expression in transfected 293T cell line by CHD4 monoclonal antibody (M01), clone 4H4.Lane 1: CHD4 transfected lysate(220 KDa).Lane 2: Non-transfected lysate.
CHD4 monoclonal antibody (M01), clone 4H4 Western Blot analysis of CHD4 expression in HeLa NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged CHD4 is approximately 0.03ng/ml as a capture antibody.
Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE)
CHD4 monoclonal antibody (M01), clone 4H4 Western Blot analysis of CHD4 expression in HeLa NE ( Cat # L013V3 ).
Human OVCAR-3; WB Suggested Anti-CHD4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: OVCAR-3 cell lysate. There is BioGPS gene expression data showing that CHD4 is expressed in OVCAR3.; CHD4 antibody - N-terminal region (ARP34372_P050) in Human OVCAR-3 cells using Western Blot
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using CHD4 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HepG2 cells, using CHD4 antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-CHD4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: OVCAR-3 cell lysateThere is BioGPS gene expression data showing that CHD4 is expressed in OVCAR3
WB was performed on nuclear extracts from HeLa cells (HeLa NE, 20 ug) using the antibody against CHD4 diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
WB was performed on nuclear extracts from HeLa cells (HeLa NE, 20 ug) using the antibody against CHD4 diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA313619 | CHD4 antibody |
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| Rabbit polyclonal anti-CHD4 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, P |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA346917 | CHD4 antibody |
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| Mouse Monoclonal CHD4 Antibody | |||||||
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Mouse | IgG2b | 5F1-A11-C9 | Purified | WB |
0.1 ml /
€335.00
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|
| OriGene Technologies, Inc. | |||||||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC224232L1V | Lenti ORF particles, CHD4 (Myc-DDK tagged) - Human chromodomain helicase DNA binding protein 4 (CHD4), 200 uL, >10^7 TU/mL |
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200 µl /
€2,420.00
|
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| OriGene Technologies, Inc. | ||