CHD5 antibody
Principal name
CHD5 antibody
Alternative names for CHD5 antibody
CHD-5, KIAA0444, Chromodomain-helicase-DNA-binding protein 5, ATP-dependent helicase CHD5
SwissProt ID
Q8TDI0 (Human)
Gene ID
26038 (CHD5)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of CHD5 antibody
- Kit Component:
- - KN217757G1, CHD5 gRNA vector 1 in pCas-Guide vector
- KN217757G2, CHD5 gRNA vector 2 in pCas-Guide vector
- KN217757D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
HeLa cells were transfected with CHD5 and analysed by Western blot.Western blot was performed using 15 to 30 µg nuclear extracts and CHD5 polyclonal antibody ( Cat # PAB14086 ) ( Dilution at 1 : 1,000 ).Lane 1 shows the result of the untransfected cells, lane 2 of the transfected cells.Western blot performed by Sonia Mulero, Spanish National Cancer Research Centre ( CNIO ), Madrid, Spain.
ELISA was performed using a serial dilution of CHD5 polyclonal antibody ( Cat # PAB14086 ).The plates were coated with the peptides used for immunization of the rabbit.By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 93,500.
Western blot analysis of CHD5 expression in Hela (A); HepG2 (B); NIH3T3 (C); rat brain (D) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CHD5 rabbit polyclonal antibody. (TA590515)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CHD5 rabbit polyclonal antibody. (TA590515)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHD5 (RC217757, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHD5.
ELISA: CHD5 Antibody
WB. WB performed by Sonia Mulero, Spanish National Cancer Research Centre (CNIO), Madrid , Spain .
Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against human CHD5. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:93,500.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA347103 | CHD5 antibody |
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| Rabbit Polyclonal CHD5 Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E |
0.1 ml /
€285.00
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC217757L1V | Lenti ORF particles, CHD5 (Myc-DDK tagged) - Human chromodomain helicase DNA binding protein 5 (CHD5), 200 uL, >10^7 TU/mL |
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200 µl /
€2,420.00
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| OriGene Technologies, Inc. | ||