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CHGN antibody

Principal name

CHGN antibody

Alternative names for CHGN antibody

CsGalNAcT-1, CSGALNACT1, GALNACT1, Beta4GalNAcT-1, Chondroitin sulfate N-acetylgalactosaminyltransferase 1, Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 1

SwissProt ID

Q8BJQ9 (Mouse), Q8TDX6 (Human)

Gene ID

55790 (CSGALNACT1), 234356 (Csgalnact1)

Ncbi ID

9606, NP_060841, NP_001123990.1, NM_001130518, NP_766341.4

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of CHGN antibody

Kit Component:: - KN303893G1, 4732435N03Rik gRNA vector 1 in pCas-Guide vector
- KN303893G2, 4732435N03Rik gRNA vector 2 in pCas-Guide vector
- KN303893D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:: CRISPR/CAS9 Genome Editing Manual

General readings

Sakai,K., (2007) J. Biol. Chem. 282 (6), 4152-4161

Immunoperoxidase of purified MaxPab antibody to ChGn on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml]

H00055790-B02

Western Blot analysis of CSGALNACT1 expression in transfected 293T cell line (H00055790-T02) by CSGALNACT1 MaxPab polyclonal antibody.Lane 1: ChGn transfected lysate(32.67 KDa).Lane 2: Non-transfected lysate.

H00055790-B02

Western Blot analysis of CSGALNACT1 expression in transfected 293T cell line (H00055790-T01) by CSGALNACT1 MaxPab polyclonal antibody.Lane 1: ChGn transfected lysate(32.78 KDa).Lane 2: Non-transfected lysate.

H00055790-B01P

Immunoperoxidase of purified MaxPab antibody to ChGn on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml]

H00055790-B02P

H00055790-B02P

Human Jurkat; WB Suggested Anti-CSGALNACT1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; CSGALNACT1 antibody - N-terminal region (ARP47434_P050) in Human Jurkat cells using Western Blot

ARP47434_P050

Hum. Fetal Heart; Host:Rabbit. Target Name:CSGALNACT1. Sample Tissue:Human Fetal Heart. Antibody Dilution: 1.0ug/ml.; ChGn antibody - C-terminal region (ARP47435_P050) in Hum. Fetal Heart cells using Western Blot

ARP47435_P050

Human MCF-7; WB Suggested Anti-ChGn Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; ChGn antibody - C-terminal region (ARP47435_P050) in Human MCF-7 cells using Western Blot

ARP47435_P050

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CSGALNACT1 Antibody. The picture on the right is treated with the synthesized peptide.

Immunohistochemical analysis of CSGALNACT1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of CSGALNACT1 expression in MCF7 (A); K562 (B) whole cell lysates.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using CSGALNACT1 antibody.The picture on the right is treated with the synthesized peptide.