CHK2 antibody
Principal name
CHK2 antibody
Alternative names for CHK2 antibody
CHEK2, CHEK-2, CHK-2, RAD53, Cds1, Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog
Ncbi ID
NP_001005735.1, NP_001005735, 9606, O96017, NM_001005735, NP_009125, NM_007194, NP_057890.1, NM_016681, NM_145862
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F), Luminex (LMNX), Frozen Sections (C)
Background of CHK2 antibody
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, CHEK2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in its gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in its gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
General readings
Clone DCS-273 is used in these references:
1. Syljuäsen, R.G., et al., Cancer Res. 66, 10253-10257 (2006)
2. Berger, M., et al., Mol. Cell Biol. 25, 5380-5388 (2005)
3. Latella, L., et al., Mol. Cell Biol. 24, 6350-6361 (2004)
4. Louria-Hayon, I., et al., J. Biol. Chem. 278, 33134-33141 (2003)
5. Lukas, C., et al., Cancer Res. 61, 4990-4993 (2001)
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Chk2 (Phospho-Thr68) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from Jurkat cells treated with UV, using Chk2 (Phospho-Thr68) Antibody. The lane on the right is treated with the synthesized peptide.
AM06003PU-N CHEK2 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Spleen at 10 µg/ml followed by Biotin anti-Mouse IgG secondary antibody, Alkaline Phosphatase-streptavidin and Chromogen.
Western blot (WB) analysis of p-Chk2 (pSer516) antibody Cat.-No.: AP01555PU-N in extracts from HeLa cells treated with UV
Figure 2. Immunohistochemistry (IHC) analyzes of p-Chk2 antibody (pThr68) in paraffin-embedded human breast carcinoma tissue. (Cat.-No.: AP01556PU-N)
Figure 1. Western blot (WB) analysis of p-Chk2 antibody (pThr68) in extracts from NIH/3T3 cells treated with UV. (Cat.-No.: AP01556PU-N)
Western blot (WB) analysis of Chk2 antibody (Cat.-No.: AP06051PU-N) at 1/500 dilution
Lane1:Hela cell lysate
Lane2:Mouse liver tissue lysate
Lane3:Rat spleen tissue lysate
Lane1:Hela cell lysate
Lane2:Mouse liver tissue lysate
Lane3:Rat spleen tissue lysate
Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using CHK2 antibody (Cat.-No. AP06052PU-N).
Western blot (WB) analysis of Chk2 antibody (Cat.-No.: AP06052PU-N) at 1/500 dilution
Lane1:MCF-7 cell lysate
Lane2:Mouse liver tissue lysate
Lane3:Rat liver tissue lysate
Lane1:MCF-7 cell lysate
Lane2:Mouse liver tissue lysate
Lane3:Rat liver tissue lysate
Immunohistochemistry (IHC) analyzes of Chk2 antibody (Cat.-No.: AP06380PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of Chk2 antibody (Cat.-No.: AP06380PU-N) in extracts from Jurkat cells.
Western blot (WB) analysis of Chk2 antibody (Cat.-No.: AP06512PU-N) in extracts from COS7 cells treated with UV 30'.
Immunohistochemistry (IHC) analyzes of p-CHK2 (pThr387) antibody (Cat.-No.: AP20931PU-N) in paraffin-embedded human lung adenocarcinoma tissue.
Immunohistochemistry analyzes of p-Chk2 antibody (pThr68) in paraffin-embedded human lung adenocarcinoma tissue
Immunohistochemistry (IHC) analyzes of CHK2 pAb in paraffin-embedded human lung adenocarcinoma tissue.
Immunohistochemistry (IHC) analyzes of CHK2 pAb in paraffin-embedded human lung adenocarcinoma tissue.
CHK2 Polyclonal Antibody
Western blot analysis of extract from HL60 cells using Chk2 Antibody AP26045PU-N.
Human Jurkat; WB Suggested Anti-CHEK2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. CHEK2 is supported by BioGPS gene expression data to be expressed in Jurkat.; CHEK2 antibody - N-terminal region (ARP30290_P050) in Human Jurkat cells using Western Blot
Human Brain; WB Suggested Anti-CHEK2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human brain; CHEK2 antibody - N-terminal region (ARP30335_P050) in Human Brain cells using Western Blot
Hum. Adult Placenta; Host:Rabbit. Target Name:CHEK2. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; CHEK2 antibody - N-terminal region (ARP30335_P050) in Hum. Adult Placenta cells using Western Blot
Hum. Fetal Muscle; Host:Rabbit. Target Name:CHEK2. Sample Tissue:Human Fetal Muscle. Antibody Dilution: 1.0ug/ml.; CHEK2 antibody - N-terminal region (ARP30335_P050) in Hum. Fetal Muscle cells using Western Blot
Immunofluorescence of purified MaxPab antibody to CHEK2 on HeLa cell. [antibody concentration 10 ug/ml]
CHEK2 MaxPab polyclonal antibody. Western Blot analysis of CHEK2 expression in human liver.
Western Blot analysis of CHEK2 expression in transfected 293T cell line (H00011200-T01) by CHEK2 MaxPab polyclonal antibody.Lane 1: CHEK2 transfected lysate(59.73 KDa).Lane 2: Non-transfected lysate.
Immunofluorescence of purified MaxPab antibody to CHEK2 on HeLa cell. [antibody concentration 10 ug/ml]
CHEK2 MaxPab polyclonal antibody. Western Blot analysis of CHEK2 expression in human liver.
Western Blot analysis of CHEK2 expression in transfected 293T cell line (H00011200-T01) by CHEK2 MaxPab polyclonal antibody.Lane 1: CHEK2 transfected lysate(59.73 KDa).Lane 2: Non-transfected lysate.
CHEK2 MaxPab rabbit polyclonal antibody. Western Blot analysis of CHEK2 expression in mouse kidney.
Western Blot analysis of CHEK2 expression in transfected 293T cell line (H00011200-T02) by CHEK2 MaxPab polyclonal antibody.Lane 1: CHEK2 transfected lysate(60.90 KDa).Lane 2: Non-transfected lysate.
CHEK2 MaxPab rabbit polyclonal antibody. Western Blot analysis of CHEK2 expression in A-431.(CHEK2 is a phosphoprotein which increases the size of the protein. The actual band size observed may differ from that predicted.)
Immunoperoxidase of monoclonal antibody to CHEK2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to CHEK2 on HeLa cell. [antibody concentration 10 ug/ml]
CHEK2 monoclonal antibody (M01), clone 4B7. Western Blot analysis of CHEK2 expression in HeLa ( Cat # L013V1 ).
CHEK2 monoclonal antibody (M01), clone 4B7 Western Blot analysis of CHEK2 expression in HeLa NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged CHEK2 is approximately 3ng/ml as a capture antibody.
CHEK2 monoclonal antibody (M02), clone 6B8 Western Blot analysis of CHEK2 expression in Jurkat ( Cat # L017V1 ).
Immunoperoxidase of monoclonal antibody to CHEK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1.5 ug/ml]
CHEK2 monoclonal antibody (M03), clone 3E10 Western Blot analysis of CHEK2 expression in Hela NE ( Cat # L013V3 ).
CHEK2 monoclonal antibody (M03), clone 3E10 Western Blot analysis of CHEK2 expression in Hela NE ( Cat # L013V3 ).
CHEK2 monoclonal antibody (M04), clone 4F10 Western Blot analysis of CHEK2 expression in Hela NE ( Cat # L013V3 ).
Immunoperoxidase of monoclonal antibody to CHEK2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5 ug/ml]
Western blot using CHEK2 (phospho T68) polyclonal antibody ( Cat # PAB10034 ) shows detection of apredominant band at ~60 kDa corresponding to phosphorylated CHEK2 ( arrowhead ) in MCF-7 whole cell lysates after treatment with doxorubicin.CHEK2 phosphorylation was induced using increasing concentrations of the DNA damaging agent doxorubicin as indicated for 24 h prior to lysate production.Personal communication, Xiao HeYang, University of Oklahoma Health Sciences Center.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CHEK2 polyclonal antibody ( Cat # PAB5293 ) .
Western blot analysis of the extracts from Jurkat cells using CHEK2 polyclonal antibody ( Cat # PAB5293 ) .
Western blot analysis of CHEK2 expression in Jurkat cell lysate.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma (A), liver carcinoma (B), breast carcinoma (C) and kiney carcinoma (D), showing nuclear localization with DAB staining using CHK2 antibody Cat.-No AM06242SU-N
Confocal immunofluorescence analysis of Hela cells using CHK2 antibody Cat.-No AM06242SU-N (green), showing nuclear localization. Red: Actin filaments have been labeled with DY-554 phalloidin.
Western blot analysis using CHK2 antibody Cat.-No AM06242SU-N against cell lysate from various cell types.
Figure 1. AM00536AF-N CHK2 antibody Immunohistochemical staining of Human Intestine Paraffin Embedded Section
Figure 2. Western blot analysis of Chk2 expression in HeLa cells (Lane 1), NIH/3T3 cells (Lane 2) and Rat-1 cells (Lane 3) using CHK2 antibody AM00536AF-N.
Figure 3. Immunoprecipitation of Chk2 from HeLa cells with normal Mouse IgG (Lane 1) or AM00536AF-N (Lane 2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with CHK2 antibody (AM00536AF-N).
Figure 1. AM20032AF-N CHK2 antibody Immunohistochemical staining of Human Intestine Paraffin Embedded Section.
Figure 2. Western blot analysis of Chk2 expression in HeLa cells (Lane 1), NIH/3T3 cells (Lane 2) and Rat-1 cells (Lane 3) using CHK2 antibody AM20032AF-N.
Figure 3. Immunoprecipitation of Chk2 from HeLa cells with normal Mouse IgG (Lane 1) or AM20032AF-N (Lane 2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with CHK2 antibody (AM20032AF-N).
Figure 1. Western blot analysis of extracts from HeLa cells using Chk2 (phospho-Ser516) antibody (AP02432PU).
Testis: Formalin-Fixed Paraffin-Embedded (FFPE)
Western blot analysis of extracts from COS7 cells treated with UV using Chk2 (Phospho-Thr383) Antibody AP55715PU-N.The lane on the right is treated with the antigen-specific peptide.
Immunofluorescence staining of methanol-fixed HeLa cells using Chk2 (Phospho-Thr383) Antibody AP55715PU-N.
Immunohistochemical analysis of CHK2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CHK2 staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of CHK2 expression in Jurkat (A); HL60 (B); HT29 (C) whole cell lysates.
Western blot analysis of CHK2 (pT432) expression in Jurkat (A); H9C2 (B) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500384)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500384)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500384)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500384)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CHEK2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunoprecipitation(IP) of CHEK2 by using TrueMab monoclonal anti-CHEK2 antibodies (Negative control: IP without adding anti-CHEK2 antibody.). For each experiment, 500ul of DDK tagged CHEK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CHEK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC201278 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CHEK2 antibody(TA500384), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CHEK2 antibody(TA500384),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500385)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500385)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500385)
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CHEK2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunoprecipitation(IP) of CHEK2 by using TrueMab monoclonal anti-CHEK2 antibodies (Negative control: IP without adding anti-CHEK2 antibody.). For each experiment, 500ul of DDK tagged CHEK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CHEK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
HEK293T cells transfected with either RC201278 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CHEK2 antibody(TA500385), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500390)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500390)
Immunofluorescent staining of HT29 cells using anti-CHEK2 mouse monoclonal antibody (TA500390).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CHEK2 monoclonal antibody.
Immunoprecipitation(IP) of CHEK2 by using TrueMab monoclonal anti-CHEK2 antibodies (Negative control: IP without adding anti-CHEK2 antibody.). For each experiment, 500ul of DDK tagged CHEK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CHEK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Flow cytometric Analysis of Jurkat cells, using anti-CHEK2 antibody(TA500390),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC201278 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CHEK2 antibody(TA500390), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500391)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500391)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500391)
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CHEK2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunoprecipitation(IP) of CHEK2 by using TrueMab monoclonal anti-CHEK2 antibodies (Negative control: IP without adding anti-CHEK2 antibody.). For each experiment, 500ul of DDK tagged CHEK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CHEK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
HEK293T cells transfected with either RC201278 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CHEK2 antibody(TA500391), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CHEK2 antibody(TA500391),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500398)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500398)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500398)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500398)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500398)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500398)
Anti-CHEK2 mouse monoclonal antibody (TA500398) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHEK2(RC201278).
Immunofluorescent staining of HT29 cells using anti-CHEK2 mouse monoclonal antibody (TA500398).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
Immunoprecipitation(IP) of CHEK2 by using TrueMab monoclonal anti-CHEK2 antibodies (Negative control: IP without adding anti-CHEK2 antibody.). For each experiment, 500ul of DDK tagged CHEK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CHEK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CHEK2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Flow cytometric Analysis of Jurkat cells, using anti-CHEK2 antibody(TA500398),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500399)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CHEK2 monoclonal antibody.
Flow cytometric Analysis of Jurkat cells, using anti-CHEK2 antibody(TA500399),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC201278 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CHEK2 antibody(TA500399), and then analyzed by flow cytometry.
Immunofluorescent staining of HT29 cells using anti-CHEK2 mouse monoclonal antibody (TA500405).
Anti-CHEK2 mouse monoclonal antibody (TA500405) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHEK2(RC201278).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CHEK2 monoclonal antibody.
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-CHEK2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
HEK293T cells transfected with either RC201278 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CHEK2 antibody(TA500405), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CHEK2 Rabbit Polyclonal antibody. (TA590397)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CHEK2 Rabbit Polyclonal antibody. (TA590397)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-CHEK2 Rabbit Polyclonal antibody. (TA590397)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CHEK2 Rabbit Polyclonal antibody. (TA590397)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CHEK2 Rabbit Polyclonal antibody. (TA590397)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CHEK2 Rabbit Polyclonal antibody. (TA590397)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CHEK2 Rabbit Polyclonal antibody. (TA590397)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC215909, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
ELISA: Chk2 Antibody
CHEK2 ELISA with 2F4 Capture (TA600006) and 4C10 Detection (TA700006) Antibodies. Substrate used: Recombinant Human CHEK2 (TP301278)
CHEK2 Luminex ELISA with 2F4 Capture (TA600006) and 4C10 Detection (TA700006) Antibodies. Substrate used: Recombinant Human CHEK2 (TP301278)
CHEK2 ELISA with 2F4 Capture (TA600006) and 4C10 Detection (TA700006) Antibodies. Substrate used: Recombinant Human CHEK2 (TP301278)
CHEK2 Luminex ELISA with 2F4 Capture (TA600006) and 4C10 Detection (TA700006) Antibodies. Substrate used: Recombinant Human CHEK2 (TP301278)
Western blot analysis of CHK2 expression in MCF7 (A); Jurkat (B) whole cell lysates.
Immunohistochemical analysis of CHK2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of CHK2 (pT68) expression in HEK293T UV-treated (A); MCF7 UV-treated (B) whole cell lysates.
Immunohistochemical analysis of CHK2 (pT68) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of Chk2 expression in K562 (A), Jurkat (B), and HL-60 (C) whole cell lysates with Chk2 antibody at 1 ug /ml.
Immunocytochemistry of Chk2 in Jurkat cells with Chk2 antibody at 1 ug/mL.
Western blot analysis of extracts from Jurkat and HeLa cells treated with etoposide 25uM 24hours, using Chk2 (Ab-68) Antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Chk2 (Ab-68) Antibody.The picture on the right is treated with the synthesized peptide.
Western blot using Affinity Purified anti-Chk2 pT68 antibody shows detection of a predominant band at ~60 kDa corresponding to phosphorylated Chk2 (arrowhead) in MCF-7 whole cell lysates after treatment with doxorubicin. Chk2 phosphorylation was induced using increasing concentrations of the DNA damaging agent doxorubicin as indicated for 24 h prior to lysate production. Personal communication, Xiao HeYang, University of Oklahoma Health Sciences Center.
Predicted band size: 61kDa. Positive control: 293T , Hela and HT-29 cell lysate . Recommended dilution: 1/200-1000
Western blot analysis of Chk2 expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts of various cell lines, using CHEK2 antibody.
Immunohistochemistry of paraffin-embedded human stomach using CHEK2 antibody at dilution of 1:100 (400x lens).
Immunofluorescence analysis of MCF7 cell using CHEK2 antibody. Blue: DAPI for nuclear staining.
293 T cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with mouse anti-Chk2 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Western Blot: Chk2 Antibody (73C175.1.1) [TA336399] - analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) [TA336399] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Western blot analysis of extracts from A549 cells, using Phospho-CHEK2-S516 antibody.
Western blot analysis of Chk2 phosphorylation expression in UV treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of the extracts from Jurkat cells untreated or treated with UV using Chk2 (Ab-68) Antibody and Chk2 (Phospho-Thr68) Antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin- embedded lung carcinoma. Left: Using Chk2 (Phospho-Thr68) Antibody; Right: The same antibody preincubated with synthesized phosphopeptide.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from COS7 cells, treated with UV (30mins), using Chk2 (Phospho-Thr383) antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Chk2 (Phospho-Thr383) antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from Jurkat cells, using Chk2 (Phospho-Thr387) Antibody (#TA312767).The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Chk2 (Phospho-Thr387) Antibody (#TA312767).The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from HeLa cells using CHK2 (phospho-Ser516) antibody.
- Quick-Filters:
- Highly Validated (7)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500384 | CHK2 antibody |
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| CHEK2 (CHK2) mouse monoclonal antibody, clone OTI2H5 (formerly 2H5) | |||||||
 |
Mouse | IgG2b | OTI2H5 | Purified | Can, Hu | F, IP, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +8 additional images |
Highly validated antibody!
|
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| TA500385 | CHK2 antibody |
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| CHEK2 (CHK2) mouse monoclonal antibody, clone OTI4C10 (formerly 4C10) | |||||||
 |
Mouse | IgG2b | OTI4C10 | Purified | Can, Hu | F, IP, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
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| TA500390 | CHK2 antibody |
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| CHEK2 (CHK2) mouse monoclonal antibody, clone OTI10G7 (formerly 10G7) | |||||||
 |
Mouse | IgG1 | OTI10G7 | Purified | Can, Hu, Mky | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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| TA500391 | CHK2 antibody |
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| CHEK2 (CHK2) mouse monoclonal antibody, clone OTI5C7 (formerly 5C7) | |||||||
 |
Mouse | IgG1 | OTI5C7 | Purified | Can, Hu | F, IP, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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| TA500398 | CHK2 antibody |
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| CHEK2 (CHK2) mouse monoclonal antibody, clone OTI5C4 (formerly 5C4) | |||||||
 |
Mouse | IgG1 | OTI5C4 | Purified | Can, Hu | F, ICC/IF, IP, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +11 additional images |
Highly validated antibody!
|
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| TA500399 | CHK2 antibody |
||||||
| CHEK2 (CHK2) mouse monoclonal antibody, clone OTI8D11 (formerly 8D11) | |||||||
 |
Mouse | IgG1 | OTI8D11 | Purified | Can, Hu, Mky | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA500405 | CHK2 antibody |
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| CHEK2 (CHK2) mouse monoclonal antibody, clone OTI2F4 (formerly 2F4) | |||||||
 |
Mouse | IgG2b | OTI2F4 | Purified | Can, Hu, Mky, Rt | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA306063 | CHK2 (N-term) antibody |
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| Rabbit Polyclonal Chk2 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA309988 | CHK2 antibody |
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| Rabbit anti-CHK2 (Phospho-Ser516) polyclonal antibody(Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
€355.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA312767 | CHK2 antibody |
||||||
| Rabbit polyclonal Chk2 (Thr387) antibody(Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA312768 | CHK2 antibody |
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| Rabbit polyclonal Chk2 (Thr383) antibody(Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, ICC/IF, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA312769 | CHK2 antibody |
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| Rabbit polyclonal Chk2 (Thr68) antibody(Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA312770 | CHK2 antibody |
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| Rabbit polyclonal Chk2 (Ab-68) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA319237 | CHK2 pThr68 antibody |
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| Rabbit polyclonal CHK2 phospho T68 antibody | |||||||
 |
Rabbit | Purified | Hu | E, WB |
0.1 mg /
€515.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA323481 | CHK2 antibody |
||||||
| Anti-CHEK2 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA325373 | CHK2 antibody |
||||||
| Rabbit Polyclonal Chk2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA325374 | CHK2 antibody |
||||||
| Rabbit Polyclonal Chk2 (Ser516) Antibody (Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA327296 | CHK2 antibody |
||||||
| CHEK2 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA328023 | CHK2 antibody |
||||||
| Mouse Monoclonal anti-Chk2 Antibody | |||||||
 |
Mouse | IgG1 | CHK2909 | Aff - Purified | Hu | WB |
50 µl /
€130.00
|
| OriGene Technologies, Inc. | |||||||
| TA333183 | CHK2 antibody |
||||||
| Phospho-CHEK2-S516 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
