CIB1 antibody
Principal name
CIB1 antibody
Alternative names for CIB1 antibody
Calmyrin, KIP, PRKDCIP, SIP2-28, CIBP, Calcium and integrin-binding protein 1, DNA-PKcs-interacting protein, Kinase-interacting protein, SNK-interacting protein 2-28
Ncbi ID
9606, NP_006375.2, NP_006375, NM_006384, NP_036000.1, NM_011870, NM_031145, NP_112407.1
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of CIB1 antibody
- Kit Component:
- - KN303343G1, Cib1 gRNA vector 1 in pCas-Guide vector
- KN303343G2, Cib1 gRNA vector 2 in pCas-Guide vector
- KN303343D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Yuan W , et al , (2006). Mol Cell Biochem. 26(22) : 8507.
Yuan , et al , (2006). J Cell Biol. 16;172(2) :169-75.
Immunoperoxidase of monoclonal antibody to CIB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml]
Immunofluorescence of monoclonal antibody to CIB1 on HeLa cell . [antibody concentration 10 ug/ml]
Western blot analysisCell lysates of HT29 (40 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CIB1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence ICC/IF analysis of CIB1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CIB1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)
Western blot analysis: Cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CIB1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1: 293T cell lysate.
Lane 2: CIB1 Transfected 293T cell lysate.
Lane 1: 293T cell lysate.
Lane 2: CIB1 Transfected 293T cell lysate.
Western blot analysis of cell lysates of HT29 ( 40 µg ) were resolved by SDS - PAGE , transferred to NC membrane and probed with CIB1 monoclonal antibody , clone 1D1 ( 1 : 1000 ) ( Cat # MAB1113 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
CIB1: 15% SDS-PAGE (3 µg)
Western Blot analysis of CIB1 expression in transfected 293T cell line (H00010519-T01) by CIB1 MaxPab polyclonal antibody.Lane 1: CIB1 transfected lysate(21.01 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical analysis of paraffin-embedded human thalamus (left) and glioma (right) tissue, showing membrane localization using CIB1 antibody Cat.-No AM06136SU-N with DAB staining.
Western blot analysis using CIB1 antibody Cat.-No AM06136SU-N against truncated CIB1 recombinant protein (Lane 1) and A431 cell lysate (Lane 2).
Western blot analysis of CIB1 expression in HEK293T (A); H9C2 (B); rat brain (C) whole cell lysates.
Host: Rabbit; Target Name: Cib1; Sample Tissue: Rat Muscle lysates; Antibody Dilution: 1.0 ug/ml
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM03117PU-N | CIB1 (1-191) antibody |
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 |
Mouse | IgG2b | 1D1 | Aff - Purified | Hu | E, ICC/IF, WB |
0.1 ml /
€330.00
|
| OriGene Technologies GmbH | |||||||
 +2 additional images |
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| AM03117PU-S | CIB1 (1-191) antibody |
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|
Mouse | IgG2b | 1D1 | Aff - Purified | Hu | E, ICC/IF, WB |
50 µl /
€220.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AM06136SU-N | CIB1 antibody |
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 |
Mouse | IgG | 5A1F5E12 | Ascites | Hu | E, P, WB |
0.1 ml /
€380.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301591 | CIB1 |
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| Recombinant protein of human calcium and integrin binding 1 (calmyrin) (CIB1) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401918 | CIB1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201834L2V | Lenti ORF particles, Cib1 (GFP-tagged) - Mouse calcium and integrin binding 1 (calmyrin) (Cib1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR201834L1V | Lenti ORF particles, Cib1 (Myc-DDK-tagged) - Mouse calcium and integrin binding 1 (calmyrin) (Cib1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR215160L2V | Lenti ORF particles, Cib1 (GFP-tagged ORF) - Rat calcium and integrin binding 1 (calmyrin) (Cib1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR215160L1V | Lenti ORF particles, Cib1 (Myc-DDK-tagged ORF) - Rat calcium and integrin binding 1 (calmyrin) (Cib1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||