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CLCN4 antibody

Principal name

CLCN4 antibody

Alternative names for CLCN4 antibody

Chloride channel protein 4, ClC-4, ClC4, CLC4, H(+)/Cl(-) exchange transporter 4, Chloride transporter ClC-4

SwissProt ID

P51793 (Human), P51794 (Rat), Q61418 (Mouse)

Gene ID

1183 (CLCN4)

Ncbi ID

9606, NP_001821, NP_001821.2, NP_001821.1, NM_001830

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Mky (Monkey), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Frozen Sections (C)

Background of CLCN4 antibody

The family of voltage-dependent chloride channels (CLCs) regulate cellular trafficking of chloride ions, a critical component of all living cells. CLCs regulate excitability in muscle and nerve cells, aid in organic solute transport and maintain cellular volume. The genes encoding human CLC-1 through CLC-7 map to chromosomes 7, 3q26, 4q32, Xp22, Xp11, 1p36 and 16p13, respectively. CLC-1 is highly expressed in skeletal muscle. Mutations in the gene encoding CLC-1 lead to myotonia, an inheritable disorder characterized by muscle stiffness and renal salt wasting. CLC-2 is highly expressed in the epithelia of several organs including lung, which suggests CLC-2 may be a possible therapeutic target for cystic fibrosis. CLC-3 expression is particularly abundant in neuronal tissue, while CLC-4 expression is evident in skeletal and cardiac muscle as well as brain. Mutations in the gene encoding CLC-5 lead to Dent’s disease, a renal disorder characterized by proteinuria and hypercalciuria. CLC-6 and CLC-7 are broadly expressed in several tissues including testes, kidney, brain and muscle.

Immunofluorescence image of cultured chick retinal amacrine ( neuronal ) cells labeled with CLCN4 polyclonal antibody ( Cat # PAB4423 ) . Data courtesy of Emily McMains, Louisiana State University .

PAB4423

Western blot of chicken brain tissue incubated with CLCN4 polyclonal antibody ( Cat # PAB4423 ) . Data courtesy of Emily McMains, Louisiana State University .

PAB4423

Western blot (WB) analysis of CLC-4 antibody (Cat.-No.: AP01391PU-N) in extracts from MCF-7 cells.

AP01391PU-N

Immunofluorescence analysis of HuvEc cells, using CLCN4 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from MCF-7 cells, using CLCN4 Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of CLCN4 expression in MDAMB453 (A) whole cell lysates.

Adult rat brain immunohistochemistry

73-260

Adult rat brain immunohistochemistry

75-260

Western blot analysis of extracts from MCF-7 cells, using CLCN4 antibody.The lane on the right is treated with the synthesized peptide.

TA313628

Western blot of chicken brain tissue incubated with CLC4 Antibody (C-term) (Cat.# TA324834). Data courtesy of Emily McMains, Louisiana State University.

TA324834

Retinae were collected from adult White Leghorn chicken were incubated at RT with 1:250 (1%BSA/.1% saponin PBS) ClC4 antibody (Cat.# TA324834) for 1 hour. Sections were then washed in PBS (3X10minutes) and then treated with secondary antibody (1:500 Cy3) for one hour. After another PBS wash series, sections were coverslipped and antibody labeling was visualized at 20X with a Leica upright microscope using a TRITC filter set and Xenon lamp illumination. (Crousillac et al, 2003)

TA324834

Immunofluorescence image of cultured chick retinal amacrine (neuronal) cells labeled with CLC4 Antibody (C-term) (Cat # TA324834). Data courtesy of Emily McMains, Louisiana State University.

TA324834

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA313628	CLCN4 antibody
	Rabbit polyclonal anti-CLCN4 antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, ICC/IF, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA324834	CLCN4 (C-term) antibody
	Rabbit polyclonal CLC4 Antibody (C-term)
	Rabbit	Ig		Purified	Hu	ICC/IF, WB	0.4 ml / €415.00
							OriGene Technologies, Inc.
+2 additional images
73-260	CLCN4 antibody
	Mouse	IgG1	N222/6	Supernatant	Hu, Ms, Rt	C, ICC/IF, WB	5 ml / €490.00
							Antibodies Incorporated

75-260	CLCN4 antibody
	Mouse	IgG1	N222/6	Purified	Hu, Ms, Rt	C, ICC/IF, WB	0.1 ml / €570.00
							Antibodies Incorporated

AP01391PU-N	CLCN4 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	ICC/IF, WB	0.1 mg / €370.00
							OriGene Technologies GmbH