CLIC1 antibody
Principal name
CLIC1 antibody
Alternative names for CLIC1 antibody
NCC27, Chloride channel ABP, hRNCC, Nuclear chloride ion channel 27, Regulatory nuclear chloride ion channel protein, Chloride intracellular channel protein 1
SwissProt ID
O00299 (Human), Q29238 (Pig), Q5E9B7 (Bovin), Q6MG61 (Rat), Q95MF9 (Rabit), Q9Z1Q5 (Mouse)
Ncbi ID
NP_001279, 9606, NP_001279.2, NP_254279.1, NM_033444, NM_001002807, NM_001288
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)
Background of CLIC1 antibody
Chloride intracellular channel (CLIC) is a family of proteins encoded by six different genes (CLIC1-6). The localization of the various CLIC channels remains elusive and controversial since they can be either found in a soluble form in the cytoplasm or as integral membrane proteins (in intracellular compartments such as the nuclear membrane or the endoplasmic reticulum for example, or associate to the plasma membrane) via their single transmembrane domain. When membrane associated, CLIC channels indeed function as ion channels (ebling the passage of Cl ions). Non-channel functions are also attributed to CLICs and include cell cycle regulation, cell differentiation, and apoptosis. These different roles depend whether CLICs are membrane bound or cytosolic. CLIC1, which was first identified in nuclear membranes, is detected in the epithelia of airways, the gall bladder, pancreas, stomach, small intestine and colon. CLIC1 was also found to be expressed in the endothelium where it plays a role in cell growth and migration.
General readings
Warton K, et al. (2002) J Biol Chem. 277(29):26003-11.
Singh H,et al. (2007) FEBS J. 274(24):6306-16.
Human HepG2; WB Suggested Anti-CLIC1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; CLIC1 antibody - C-terminal region (ARP35044_P050) in Human HepG2 cells using Western Blot
CLIC1 monoclonal antibody (M01), clone 2D4 Western Blot analysis of CLIC1 expression in HL-60 ( Cat # L014V1 ).
Immunoperoxidase of monoclonal antibody to CLIC1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to CLIC1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.15 ug/ml]
Western Blot analysis of CLIC1 expression in transfected 293T cell line by CLIC1 monoclonal antibody (M02), clone 3F9.Lane 1: CLIC1 transfected lysate(26.9 KDa).Lane 2: Non-transfected lysate.
CLIC1 monoclonal antibody (M02), clone 3F9 Western Blot analysis of CLIC1 expression in 293 ( Cat # L026V1 ).
Human; . Sample Type: Purified Recombinant CLIC-1 protein. Primary Dilution: 1:1000.; CLIC1 antibody - C-terminal region (ARP35044_T100) in Human cells using Western Blot
Mouse renal epithelial; . Lanes: Lane 1: 30ug mouse renal epithelial lysate. Lane 2: 30ug mouse renal epithelial lysate. Lane 3: 30ug mouse renal epithelial lysate. Primary Antibody Dilution: 1:500. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:2500. Gene Name: CLIC1. Submitted by: Dr. JUNMING FAN,ECU.; CLIC1 antibody - C-terminal region (ARP35044_T100) in Mouse renal epithelial cells using Western Blot
Human; CLIC1 antibody - C-terminal region (ARP35044_T100) validated by WB using purified recombinant CLIC1 protein and transfected lysate; CLIC1 antibody - C-terminal region (ARP35044_T100) in Human cells using Western Blot
Human kidney; CLIC1 antibody - N-terminal region (ARP35045_T100) in Human kidney cells using Immunohistochemistry
Mouse renal epithelial; . Lanes: Lane 1: 30ug mouse renal epithelial lysate. Lane 2: 30ug mouse renal epithelial lysate. Lane 3: 30ug mouse renal epithelial lysate. Primary Antibody Dilution: 1:500. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:2500. Gene Name: CLIC1. Submitted by: Dr. JUNMING FAN,ECU.; CLIC1 antibody - N-terminal region (ARP35045_T100) in Mouse renal epithelial cells using Western Blot
Human Jurkat; WB Suggested Anti-CLIC1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; CLIC1 antibody - N-terminal region (ARP35045_T100) in Human Jurkat cells using Western Blot
Human; Sample Type: Purified Recombinant CLIC-1 Protein Primary Dilution: 1:1000Submitted By: Harpreet Singh, University of California Los Angeles; CLIC1 antibody - N-terminal region (ARP35045_T100) in Human cells using Western Blot
Immunoperoxidase of monoclonal antibody to CLIC1 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 3ug/ml]
CLIC1 monoclonal antibody (M01), clone 2D4 Western Blot analysis of CLIC1 expression in HL-60 ( Cat # L014V1 ).
Immunofluorescence of purified MaxPab antibody to CLIC1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of CLIC1 expression in transfected 293T cell line (H00001192-T02) by CLIC1 MaxPab polyclonal antibody.Lane 1: CLIC1 transfected lysate(26.90 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CLIC1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-CLIC1 Antibody in A375 cell line lysates (35ug/lane). CLIC1(arrow) was detected using the purified Pab (1:60dilution).
Immunofluorescence of purified MaxPab antibody to CLIC1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of CLIC1 expression in transfected 293T cell line (H00001192-T02) by CLIC1 MaxPab polyclonal antibody.Lane 1: CLIC1 transfected lysate(26.90 KDa).Lane 2: Non-transfected lysate.
Recombinant human CLIC1, 1-241 aa, His-tagged: 15% SDS-PAGE (3 ug)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
CLIC1 monoclonal antibody, clone 2D4 Western Blot analysis of CLIC1 expression in HL-60.
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot analysis of CLIC1 expression in transfected 293T cell line by CLIC1 monoclonal antibody, clone 3F9.
CLIC1 monoclonal antibody, clone 3F9 Western Blot analysis of CLIC1 expression in 293.
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
TA326744 (2µg/ml) staining of U937 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA326745 (2µg/ml) staining of U937 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CLIC1 Antibody (Center) (Cat. #TA328109) western blot analysis in HL-60,MCF-7,Raji,WiDr cell line and human placenta tissue lysates (35ug/lane).This demonstrates the CLIC1 antibody detected the CLIC1 protein (arrow).
Western blot analysis of human colorectal adenocarcinoma HT-29 (lanes 1 and 5), T-84 (lanes 2 and 6), human pancreatic carcinoma PANC-1 (lanes 3 and 7) and rat kidney (lanes 4 and 8) lysates: 1- 4. Anti-CLIC1 antibody , (1:200). 5- 8. Anti-CLIC1 antibody, preincubated with the control peptide antigen.
Expression of CLIC1 human colorectal adenocarcinoma HT-29 cells. Immunocytochemical staining of fixed and permeabilized human colorectal adenocarcinoma HT-29 cells. A. Cells were stained with Anti-CLIC1 antibody , (1:200), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Merge of the two images.
WB Suggested Anti-CLIC1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
Lanes: ; Lane 1: 30ug mouse renal epithelial lysate; Lane 2: 30ug mouse renal epithelial lysate; Lane 3: 30ug mouse renal epithelial lysate; Primary Antibody Dilution: ; 1:500; Secondary Antibody: ; Anti-rabbit-HRP; Secondary Antibody Dilution: ; 1:2500;
CLIC1 antibody - C-terminal region validated by WB using purified recombinant CLIC1 protein and transfected lysate
Sample Type: Purified Recombinant CLIC-1 proteinPrimary Dilution: 1:1000
Lanes: ; Lane 1: 30ug mouse renal epithelial lysate; Lane 2: 30ug mouse renal epithelial lysate; Lane 3: 30ug mouse renal epithelial lysate; Primary Antibody Dilution: ; 1:500; Secondary Antibody: ; Anti-rabbit-HRP; Secondary Antibody Dilution: ; 1:2500;
WB Suggested Anti-CLIC1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate
Sample Type: Purified Recombinant CLIC-1 Protein Primary Dilution: 1:1000Submitted By: Harpreet Singh, University of California Los Angeles
Human kidney
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Human testis tissue, Primary antibody: (CLIC1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using (CLIC1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (CLIC1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
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- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM20871PU-N | CLIC1 antibody |
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Mouse | IgG2a | 3F9 | Hu | E, P, WB |
50 µg /
€475.00
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| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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