CNOT2 antibody
Principal name
CNOT2 antibody
Alternative names for CNOT2 antibody
CDC36, NOT2, HSPC131, MSTP046, CCR4-NOT transcription complex subunit 2, CCR4-associated factor 2
Ncbi ID
9606, NP_055330, NP_001186231.1, NP_001032935.2, BC063105, NM_028082, NM_001037848, NM_001037846, NM_014515
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)
Background of CNOT2 antibody
- Kit Component:
- - KN303560G1, Cnot2 gRNA vector 1 in pCas-Guide vector
- KN303560G2, Cnot2 gRNA vector 2 in pCas-Guide vector
- KN303560D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Albert T.K., Lemaire M., van Berkum N.L., Gentz R., Collart M.A., Timmers H.T.M. Nucleic Acids Res. 28:809-817(2000)
CNOT2 monoclonal antibody (M08), clone 3F1 Western Blot analysis of CNOT2 expression in A-549 ( Cat # L025V1 ).
Human Liver; WB Suggested Anti-CNOT2 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:12500. Positive Control: Human Liver; CNOT2 antibody - middle region (ARP32048_T100) in Human Liver cells using Western Blot
Detection limit for recombinant GST tagged CNOT2 is approximately 3ng/ml as a capture antibody.
CNOT2 monoclonal antibody (M08), clone 3F1 Western Blot analysis of CNOT2 expression in A-549 ( Cat # L025V1 ).
Western Blot analysis of CNOT2 expression in transfected 293T cell line (H00004848-T01) by CNOT2 MaxPab polyclonal antibody.Lane 1: CNOT2 transfected lysate(59.70 KDa).Lane 2: Non-transfected lysate.
Human THP-1; WB Suggested Anti-CNOT2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; CNOT2 antibody - middle region (ARP32721_P050) in Human THP-1 cells using Western Blot
Immunohistochemical analysis of paraffin-embedded human brain tissue using CNOT2 (Phospho-Ser101) antibody AP55827PU-N (left)or the same antibody preincubated with blocking peptide (right).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CNOT2 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808626)(1:150)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CNOT2 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808626)(1:150)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CNOT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808626)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CNOT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808626)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT2 (RC204121, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT2.(1:2000)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CNOT2 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808695)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CNOT2 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808695)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CNOT2 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808695)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CNOT2 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808696)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CNOT2 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808696)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT2 (RC204121, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT2.(1:2000)
Western blot analysis of extracts from HepG2 cells, treated with starved (24hours), using CNOT2 (Ab-101) antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-CNOT2 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:12500; Positive Control: Human Liver
WB Suggested Anti-CNOT2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: THP-1 cell lysate
Immunohistochemistry analysis of paraffin-embedded human brain tissue using CNOT2 (Phospho-Ser101) antibody.The picture on the right is treated with the synthesized peptide.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA808626 | CNOT2 antibody |
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| CNOT2 mouse monoclonal antibody, clone OTI5A12 (formerly 5A12) | |||||||
 |
Mouse | IgG2b | OTI5A12 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody!
|
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| TA314275 | CNOT2 antibody |
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| Rabbit polyclonal CNOT2 (Ab-101) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA314276 | CNOT2 antibody |
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| Rabbit polyclonal CNOT2 (Ser101) antibody(Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA808695 | CNOT2 antibody |
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| CNOT2 mouse monoclonal antibody, clone OTI6D7 (formerly 6D7) | |||||||
 |
Mouse | IgG2a | OTI6D7 | Purified | Hu | P |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA808696 | CNOT2 antibody |
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| CNOT2 mouse monoclonal antibody, clone OTI9B10 (formerly 9B10) | |||||||
 |
Mouse | IgG2b | OTI9B10 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AP55827PU-N | CNOT2 pSer101 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P |
0.1 mg /
€300.00
|
||
| OriGene Technologies GmbH | |||||||
| AP55827PU-S | CNOT2 pSer101 antibody |
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|
Rabbit | Aff - Purified | Hu, Ms, Rt | P |
50 µg /
€230.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760106 | CNOT2 (full length, N-term HIS tag) |
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| Recombinant protein of human CCR4-NOT transcription complex, subunit 2 (CNOT2), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208509L2V | Lenti ORF particles, Cnot2 (GFP-tagged) - Mouse CCR4-NOT transcription complex, subunit 2 (cDNA clone MGC:70296 IMAGE:5003026), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR208509L1V | Lenti ORF particles, Cnot2 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 2 (cDNA clone MGC:70296 IMAGE:5003026), 200 uL, >10^7 TU/mL |
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|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||