CNOT4 antibody
Principal name
CNOT4 antibody
Alternative names for CNOT4 antibody
NOT4Hp, NOT4, CCR4-NOT transcription complex subunit 4, E3 ubiquitin-protein ligase CNOT4, CCR4-associated factor 4, Potential transcriptional repressor NOT4Hp
Ncbi ID
9606, NP_001008226, NP_001008226.1, NM_013316, NP_037448, NP_001157883.1, NM_001164412, NM_001164413, NM_016877, NM_001164411, NM_001164414, NM_001008225, NM_001190848, NM_001190849, NM_001190850
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)
Background of CNOT4 antibody
- Kit Component:
- - KN303562G1, Cnot4 gRNA vector 1 in pCas-Guide vector
- KN303562G2, Cnot4 gRNA vector 2 in pCas-Guide vector
- KN303562D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot analysis of CNOT4 ( arrow ) using rabbit CNOT4 polyclonal antibody ( Cat # PAB3119 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the CNOT4 gene ( Lane 2 ) ( Origene Technologies ) .
Western blot analysis of CNOT4 polyclonal antibody ( Cat # PAB3120 ) in CEM cell line lysates ( 35 µg/lane ) . CNOT4 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Western blot analysis of CNOT4 Antibody (C-term) (AP11452PU-N) in CEM cell line lysates (35 µg/lane). CNOT4 (arrow) was detected using the purified Pab (1:60 dilution).
Human NCI-H226; WB Suggested Anti-CNOT4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: NCI-H226 cell lysate. CNOT4 is strongly supported by BioGPS gene expression data to be expressed in NCI-H226.; CNOT4 antibody - middle region (ARP57877_P050) in Human NCI-H226 cells using Western Blot
Human HEK293T; WB Suggested Anti-CNOT4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate. CNOT4 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.; CNOT4 antibody - N-terminal region (ARP34307_P050) in Human HEK293T cells using Western Blot
Immunohistochemistry (IHC) analyzes of CNOT4 antibody (Cat.-No.: AP21144PU-N) in paraffin-embedded human testis tissue.
Western blot (WB) analysis of CNOT4 antibody (Cat.-No.: AP21144PU-N) in extracts from PC12 cells.
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of CNOT4 in A549 lysate with CNOT4 antibody (AP22977PU-N) at (A) 1 and (B) 2 µg/ml.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CNOT4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Flow cytometric Analysis of Hela cells, using anti-CNOT4 antibody(TA504447),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-CNOT4 antibody(TA504447),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4.
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-CNOT4 Mouse monoclonal antibody. (TA800048; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-CNOT4 Mouse monoclonal antibody. (TA800048; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CNOT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800157)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CNOT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800157)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CNOT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800157)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CNOT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800157)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CNOT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800157)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CNOT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800157)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CNOT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800157)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CNOT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800157)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CNOT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800157)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CNOT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800157)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CNOT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800157)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4.
Western blot analysis of CNOT4 (arrow) using rabbit polyclonal CNOT4 Antibody (N-term) (RB11916). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CNOT4 gene (Lane 2) (Origene Technologies).
CNOT4 Antibody (N-term) (Cat. #TA302282) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CNOT4 antibody detected the CNOT4 protein (arrow).
Western blot analysis of CNOT4 in A549 lysate with CNOT4 antibody at (A) 1 and (B) 2 µg/ml.
Western Blot: CNOT4 Antibody [TA336994] - analysis of CNOT4 in human skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide using CNOT4 antibody at 0.05 ug/ml.
Immunohistochemistry-Paraffin: CNOT4 Antibody [TA336994] - Formalin-fixed paraffin-embedded human prostate tissue stained with CNOT4 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate bu
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA800157 | CNOT4 antibody |
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| CNOT4 mouse monoclonal antibody, clone OTI1H2 (formerly 1H2) | |||||||
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Mouse | IgG1 | OTI1H2 | Purified | Hu | P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +11 additional images |
Highly validated antibody!
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| TA302282 | CNOT4 (N-term) antibody |
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| Rabbit Polyclonal Antibody against CNOT4 (N-term) | |||||||
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Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA306651 | CNOT4 antibody |
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| Rabbit Polyclonal CNOT4 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA336994 | CNOT4 antibody |
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| Rabbit Polyclonal CNOT4 Antibody | |||||||
![Western Blot: CNOT4 Antibody [TA336994] - analysis of CNOT4 in human skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide using CNOT4 antibody at 0.05 ug/ml.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-24527-1-W.jpg) |
Rabbit | Purified | Bov, Chk, Hu, Ms, Rt, Xen | P, WB |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA504447 | CNOT4 antibody |
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| CNOT4 mouse monoclonal antibody, clone OTI3D11 (formerly 3D11) | |||||||
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Mouse | IgG1 | OTI3D11 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA504472 | CNOT4 antibody |
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| CNOT4 mouse monoclonal antibody, clone OTI3A3 (formerly 3A3) | |||||||
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Mouse | IgG1 | OTI3A3 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA504473 | CNOT4 antibody |
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| CNOT4 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5) | |||||||
 |
Mouse | IgG1 | OTI2A5 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA504474 | CNOT4 antibody |
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| CNOT4 mouse monoclonal antibody, clone OTI4B2 (formerly 4B2) | |||||||
 |
Mouse | IgG1 | OTI4B2 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA504475 | CNOT4 antibody |
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| CNOT4 mouse monoclonal antibody, clone OTI4C7 (formerly 4C7) | |||||||
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Mouse | IgG1 | OTI4C7 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| TA800009 | CNOT4 antibody |
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| CNOT4 mouse monoclonal antibody, clone OTI2H4 (formerly 2H4) | |||||||
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Mouse | IgG1 | OTI2H4 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA800047 | CNOT4 antibody |
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| CNOT4 mouse monoclonal antibody, clone OTI3B8 (formerly 3B8) | |||||||
 |
Mouse | IgG1 | OTI3B8 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA800048 | CNOT4 antibody |
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| CNOT4 mouse monoclonal antibody, clone OTI1F10 (formerly 1F10) | |||||||
 |
Mouse | IgG1 | OTI1F10 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA800049 | CNOT4 antibody |
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| CNOT4 mouse monoclonal antibody, clone OTI2F10 (formerly 2F10) | |||||||
 |
Mouse | IgG1 | OTI2F10 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| TA800050 | CNOT4 antibody |
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| CNOT4 mouse monoclonal antibody, clone OTI1F9 (formerly 1F9) | |||||||
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Mouse | IgG1 | OTI1F9 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA800051 | CNOT4 antibody |
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| CNOT4 mouse monoclonal antibody, clone OTI1G3 (formerly 1G3) | |||||||
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Mouse | IgG1 | OTI1G3 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA800052 | CNOT4 antibody |
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| CNOT4 mouse monoclonal antibody, clone OTI7D7 (formerly 7D7) | |||||||
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Mouse | IgG1 | OTI7D7 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AP11452PU-N | CNOT4 (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| AP21144PU-N | CNOT4 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP22977PU-N | CNOT4 (N-term) antibody |
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 |
Rabbit | Purified | Hu, Ms, Rt | P, WB |
50 µg /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307541 | CNOT4 (transcript variant 2) |
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| Recombinant protein of human CCR4-NOT transcription complex, subunit 4 (CNOT4), transcript variant 2 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||