CoA synthase antibody
Principal name
CoA synthase antibody
Alternative names for CoA synthase antibody
Coenzyme A synthase, NBP, POV-2, COASY, Bifunctional coenzyme A synthase
Ncbi ID
9606, NP_001035994, NP_001035994.1, NM_025233, NM_001042529, NP_079509, NP_082172.2, NM_001042530
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of CoA synthase antibody
- Kit Component:
- - KN303600G1, Coasy gRNA vector 1 in pCas-Guide vector
- KN303600G2, Coasy gRNA vector 2 in pCas-Guide vector
- KN303600D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot analysis of COASY over-expressed 293 cell line, cotransfected with COASY Validated Chimera RNAi ( Cat # H00080347-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with COASY monoclonal antibody (M01), clone 1H6 (Cat # H00080347-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of COASY expression in transfected 293T cell line by COASY monoclonal antibody (M01), clone 1H6.Lane 1: COASY transfected lysate(30.2 KDa).Lane 2: Non-transfected lysate.
COASY monoclonal antibody (M01), clone 1H6 Western Blot analysis of COASY expression in A-431 ( Cat # L015V1 ).
Detection limit for recombinant GST tagged COASY is approximately 0.1ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to COASY on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged COASY is approximately 1ng/ml as a capture antibody.
COASY polyclonal antibody (A01), Lot # 060613JCS1. Western Blot analysis of COASY expression in HepG2.
COASY MaxPab polyclonal antibody. Western Blot analysis of COASY expression in human liver.
Western Blot analysis of COASY expression in transfected 293T cell line (H00080347-T01) by COASY MaxPab polyclonal antibody.Lane 1: COASY transfected lysate(29.59 KDa).Lane 2: Non-transfected lysate.
COASY MaxPab rabbit polyclonal antibody. Western Blot analysis of COASY expression in mouse spleen.
Western Blot analysis of COASY expression in transfected 293T cell line (H00080347-T01) by COASY MaxPab polyclonal antibody.Lane 1: COASY transfected lysate(30.20 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of COASY expression in transfected 293T cell line (H00080347-T01) by COASY MaxPab polyclonal antibody.Lane 1: COASY transfected lysate(30.20 KDa).Lane 2: Non-transfected lysate.
COASY MaxPab rabbit polyclonal antibody. Western Blot analysis of COASY expression in mouse spleen.
Immunoprecipitation of COASY transfected lysate using anti-COASY MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with COASY MaxPab mouse polyclonal antibody (B01) (H00080347-B01).
Western blot analysis of COASY expression in HeLa (A); A431 (B); Raw264.7 (C) whole cell lysates.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COASY (RC220733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COASY.
Flow cytometric Analysis of Hela cells, using anti-COASY antibody(TA503060),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-COASY antibody(TA503060),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503343)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503343)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COASY (RC220733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COASY.
HEK293T cells transfected with either RC220733 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-COASY antibody(TA503343), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503344)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503344)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503344)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503344)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503344)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503344)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503344)
Anti-COASY mouse monoclonal antibody (TA503344) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY COASY(RC220733).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COASY (RC220733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COASY.
HEK293T cells transfected with either RC220733 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-COASY antibody(TA503344), and then analyzed by flow cytometry.
WB Suggested Anti-COASY Antibody; Titration: 1.0 ug/ml; Positive Control: A549 Whole Cell
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503343 | CoA synthase antibody |
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| COASY mouse monoclonal antibody, clone OTI2H1 (formerly 2H1) | |||||||
 |
Mouse | IgG1 | OTI2H1 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| TA503344 | CoA synthase antibody |
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| COASY mouse monoclonal antibody, clone OTI3B3 (formerly 3B3) | |||||||
 |
Mouse | IgG2a | OTI3B3 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +9 additional images |
Highly validated antibody!
|
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| TA503060 | CoA synthase antibody |
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| COASY mouse monoclonal antibody, clone OTI3D5 (formerly 3D5) | |||||||
 |
Mouse | IgG2b | OTI3D5 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP315228 | CoA synthase (transcript variant 2) |
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| Recombinant protein of human Coenzyme A synthase (COASY), nuclear gene encoding mitochondrial protein, transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP320733 | CoA synthase (transcript variant 1) |
||||
| Recombinant protein of human Coenzyme A synthase (COASY), nuclear gene encoding mitochondrial protein, transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY403068 | COASY overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY420963 | COASY overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. | ||
| LY425766 | COASY overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |