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COG2 antibody

Principal name

COG2 antibody

Alternative names for COG2 antibody

COG complex subunit 2, LDLC, Conserved oligomeric Golgi complex subunit 2, Component of oligomeric Golgi complex 2, Low density lipoprotein receptor defect C-complementing protein

SwissProt ID

Q14746 (Human), Q921L5 (Mouse)

Gene ID

22796 (COG2), 76332 (Cog2)

Ncbi ID

9606, NP_031383, NP_031383.1, NP_084022.2, NM_029746, NM_007357

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Ze (Zebrafish), Bov (Bovine), Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F)

Background of COG2 antibody

Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Several complexes have been identified, including the Golgi transport complex (GTC), the LDLC complex, which is involved in glycosylation reactions, and the SEC34 complex, which is involved in vesicular transport. These 3 complexes are identical and have been termed the conserved oligomeric Golgi (COG) complex, which includes COG2. Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Several complexes have been identified, including the Golgi transport complex (GTC), the LDLC complex, which is involved in glycosylation reactions, and the SEC34 complex, which is involved in vesicular transport. These 3 complexes are identical and have been termed the conserved oligomeric Golgi (COG) complex, which includes COG2 (Ungar et al., 2002 [PubMed 11980916]).[supplied by OMIM].

General readings

Sohda,M., (2007) Traffic 8 (3), 270-284

Western blot analysis of COG2 over-expressed 293 cell line, cotransfected with COG2 Validated Chimera RNAi ( Cat # H00022796-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with COG2 monoclonal antibody (M09), clone 4C8 (Cat # H00022796-M09 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

H00022796-M09

Western Blot analysis of COG2 expression in transfected 293T cell line by COG2 monoclonal antibody (M09), clone 4C8.Lane 1: COG2 transfected lysate(83.2 KDa).Lane 2: Non-transfected lysate.

H00022796-M09

COG2 polyclonal antibody (A01), Lot # 070226JCSa. Western Blot analysis of COG2 expression in Raw 264.7.

H00022796-A01

COG2 polyclonal antibody (A01), Lot # 070226JCSa Western Blot analysis of COG2 expression in Hela NE ( Cat # L013V3 ).

H00022796-A01

COG2 polyclonal antibody (A01), Lot # 070226JCSa. Western Blot analysis of COG2 expression in PC-12.

H00022796-A01

Western Blot analysis of COG2 expression in transfected 293T cell line (H00022796-T01) by COG2 MaxPab polyclonal antibody.Lane 1: COG2 transfected lysate(81.18 KDa).Lane 2: Non-transfected lysate.

H00022796-B01

COG2 monoclonal antibody (M01), clone 3H8. Western Blot analysis of COG2 expression in IMR-32 ( Cat # L008V1 ).

H00022796-M01

Western Blot analysis of COG2 expression in transfected 293T cell line (H00022796-T01) by COG2 MaxPab polyclonal antibody.Lane 1: COG2 transfected lysate(81.18 KDa).Lane 2: Non-transfected lysate.

H00022796-B01P

Western Blot analysis of COG2 expression in transfected 293T cell line (H00022796-T02) by COG2 MaxPab polyclonal antibody.Lane 1: COG2 transfected lysate(83.20 KDa).Lane 2: Non-transfected lysate.

H00022796-D01P

Human HepG2; WB Suggested Anti-COG2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate. COG2 is supported by BioGPS gene expression data to be expressed in HepG2.; COG2 antibody - N-terminal region (ARP54815_P050) in Human HepG2 cells using Western Blot

ARP54815_P050

COG2 Antibody (N-term) (AP51004PU-N) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COG2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

AP51004PU-N

Western blot analysis of COG2 Antibody (N-term) (Cat. #AP51004PU-N) in Hela cell line lysates (35µg/lane). COG2 (arrow) was detected using the purified Pab.

AP51004PU-N

COG2 Antibody (N-term) (Cat. #AP51004PU-N) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

AP51004PU-N

TP304855

WB Suggested Anti-COG2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HepG2 cell lysateCOG2 is supported by BioGPS gene expression data to be expressed in HepG2

TA334420

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
AP51004PU-N	COG2 (N-term) antibody
	Rabbit	Ig	Aff - Purified	Hu	F, P, WB	0.4 ml / €415.00
						OriGene Technologies GmbH
+2 additional images

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP304855	COG2 (transcript variant 1)
	Recombinant protein of human component of oligomeric golgi complex 2 (COG2), transcript variant 1
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR210339L2V	Lenti ORF particles, Cog2 (GFP-tagged) - Mouse component of oligomeric golgi complex 2 (Cog2), 200 uL, >10^7 TU/mL
		200 µl / €1,520.00
		OriGene Technologies, Inc.

MR210339L1V	Lenti ORF particles, Cog2 (Myc-DDK-tagged) - Mouse component of oligomeric golgi complex 2 (Cog2), 200 uL, >10^7 TU/mL
		200 µl / €1,360.00
		OriGene Technologies, Inc.

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