COL20A1 antibody
Principal name
COL20A1 antibody
Alternative names for COL20A1 antibody
COL20A1, KIAA1510, Collagen alpha-1 XX chain
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB)
Background of COL20A1 antibody
- Kit Component:
- - KN303626G1, Col20a1 gRNA vector 1 in pCas-Guide vector
- KN303626G2, Col20a1 gRNA vector 2 in pCas-Guide vector
- KN303626D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Detection limit for recombinant GST tagged COL20A1 is approximately 0.03ng/ml as a capture antibody.
Immunohistochemical analysis of Collagen 20 alpha 1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Collagen 20 alpha 1 staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Collagen 20 alpha 1 expression in HT29 (A); NCIH292 (B) whole cell lysates.
Western blot analysis of extracts from HUVEC cells, using Collagen XX a1 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Collagen XX a1 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of NIH/3T3 cells, using Collagen XX a1antibody.The picture on the right is treated with the synthesized peptide.
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (COL20A1 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using (COL20A1 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315679 | COL20A1 antibody |
||||||
| Rabbit polyclonal Collagen XX a1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|||||||
| TA349818 | COL20A1 antibody |
||||||
| Rabbit Polyclonal Anti-COL20A1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY412244 | COL20A1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
