Collagen type V antibody
Principal name
Collagen type V antibody
Alternative names for Collagen type V antibody
COL5A1
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Radioimmunoassay (R), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Dot blot (Dot), Immunoelectron Microscopy (EM)
Background of Collagen type V antibody
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Extensively purified collagens are used for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.
Staining of FFPE Human lung parenchyma (10x and 40x) with Collagen type V Antibody Cat.-No AF6110 (Clone V-3C9) at 2 µg/ml. Antibody positive in epithelial cells of basement membrane and in alveolar interstitium.
Anti Collagen type V Antibody (Cat.-No R1042 Lot 22063, 1:200, 45 min RT) showed strong staining in FFPE sections of human lung (left) with strong staining within alveoli, vessels, and in connective tissue spaces; and placenta (right) with strong staining observed in stromal and connective tissue spaces and vessel walls. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA
Anti-Collagen type V Antibody (Cat.-No R1042 Lot 22063, 1/200, 45 min RT) showed strong staining in FFPE sections of Human lung (left) with strong staining within alveoli, vessels, and in connective tissue spaces; and placenta (right) with strong staining observed in stromal and connective tissue spaces and vessel walls. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA
COL5A1 monoclonal antibody (M01), clone 2F4 Western Blot analysis of COL5A1 expression in HeLa NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged COL5A1 is approximately 3ng/ml as a capture antibody.
Human Kidney (formalin-fixed, paraffin-embedded) stained with COL5A1 antibody AP09015BT-N at 1.25 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry (IHC) analyzes of COL5A1 antibody (Cat.-No.: AP20478PU-N) in paraffin-embedded human heart tissue.
Western blot (WB) analysis of COL5A1 antibody (Cat.-No.: AP20478PU-N) in extracts from HeLa cells.
Human MCF-7; WB Suggested Anti-COL5A1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; COL5A1 antibody - N-terminal region (ARP60015_P050) in Human MCF-7 cells using Western Blot
COL5A1 antibody (N-term) (Cat. #AP51017PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COL5A1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
COL5A1 Antibody (N-term) (Cat. #AP51017PU-N) western blot analysis in mouse bladder tissue lysates (35µg/lane).This demonstrates the COL5A1 antibody detected the COL5A1 protein (arrow).
COL5A1 Antibody (N-term) (Cat. #AP51017PU-N) western blot analysis in U251 cell line lysates (35µg/lane).This demonstrates the COL5A1 antibody detected the COL5A1 protein (arrow).
Collagen V alpha-1 chain N-propeptide-NC2 domain (COL5A1) antibody (18G5) Cat.-No AM10158PU-N
staining of human formalin-fixed paraffin-embedded skin after heat induced antigen retrieval.
staining of human formalin-fixed paraffin-embedded skin after heat induced antigen retrieval.
Collagen V alpha-1 chain N-propeptide -NC2 domain antibody (13F6) (Cat.-No AM10159PU-N) Anti-COL5A1 antibody ICC staining of human derma fibroblasts (24h).
WB : Collagen V alpha-1 chain N-propeptide -NC2 domain antibody (13F6) Cat.-No AM10159PU-N staining of reombinant pNalpha1 homotrimer (1) and pepsinized collagen V heterotrimer (2).
Collagen V alpha-1 chain N-propeptide -NC2 domain antibody (13F6) Cat.-No AM10159PU-N staining of human formalin-fixed paraffin-embedded tissue skin after heat induced antigen retrieval.
Collagen V alpha-1 chain N-propeptide -VR domain (COL5A1) antibody (6A7) Cat.-No AM10160PU-N EM staining of human dermal fibroblasts cells (5 days).
Collagen V alpha-1 chain N-propeptide -VR domain (COL5A1) antibody (6A7) Cat.-No AM10160PU-N ICC staining of human dermal fibroblasts cells (24h).
Collagen V alpha-1 chain N-propeptide -VR domain (COL5A1) antibody (6A7) (Cat.-No AM10160PU-N) staining of recombinant pNalpha1 homotrimer (1) and pepsinized collagen V heterotrimer (2).
Collagen V alpha-1 chain N-propeptide-VR domain (COL5A1) antibody (6A7) Cat.-No AM10160PU-N staining of human skin. Immunohistochemistry of formalin-fixed paraffin-embedded tissue after heat induced antigen retrieval.
Immunohistochemical analysis of Collagen 5 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Collagen 5 alpha 1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Collagen 5 alpha 1 expression in HeLa (A) whole cell lysates.
Western blot analysis of extracts from HeLa cells, using Collagen V a1 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human heart tissue using Collagen V a1 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Collagen V a1 antibody.The picture on the right is treated with the synthesized peptide.
Predicted band size: 184 kDa . Positive control: Lung tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
WB Suggested Anti-COL5A1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: MCF7 cell lysate
Western blot analysis on HeLa cell lysate using Collagen Valpha 1 Antibody
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN212443 | COL5A1 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||