COMT antibody
Principal name
COMT antibody
Alternative names for COMT antibody
Catechol O-methyltransferase
Ncbi ID
9606, NP_000745, NP_000745.1, NM_000754, NP_009294, NP_009294.1, NM_012531, NM_007310, NM_001135161, NM_001135162
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of COMT antibody
- Kit Component:
- - KN201275G1, COMT gRNA vector 1 in pCas-Guide vector
- KN201275G2, COMT gRNA vector 2 in pCas-Guide vector
- KN201275D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Cotton NJ., et al. (2004) J Biol Chem. 279(22):23710-8.
Dawling S., et al. (2001) Cancer Res. 61(18):6716-22.
Western Blot analysis of COMT expression in transfected 293T cell line by COMT monoclonal antibody (M01), clone 1G4-1A1.Lane 1: COMT transfected lysate(24.4 KDa).Lane 2: Non-transfected lysate.
COMT monoclonal antibody (M01), clone 1G4-1A1 Western Blot analysis of COMT expression in A-431 ( Cat # L015V1 ).
COMT MaxPab polyclonal antibody. Western Blot analysis of COMT expression in human placenta.
Western Blot analysis of COMT expression in transfected 293T cell line (H00001312-T01) by COMT MaxPab polyclonal antibody.Lane 1: COMT transfected lysate(24.31 KDa).Lane 2: Non-transfected lysate.
COMT polyclonal antibody ( Cat # PAB6722 ) ( 1 µg/ml ) staining of human testis lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
COMT polyclonal antibody ( Cat # PAB7078 ) ( 0.03 µg/ml ) staining of human testis lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
COMT MaxPab rabbit polyclonal antibody. Western Blot analysis of COMT expression in mouse liver.
Western Blot analysis of COMT expression in transfected 293T cell line (H00001312-T01) by COMT MaxPab polyclonal antibody.Lane 1: COMT transfected lysate(24.40 KDa).Lane 2: Non-transfected lysate.
Recombinant human COMT, 51-271 aa: 15% SDS-PAGE (3 µg)
Staining of Human testis lysate (35µg protein in RIPA buffer) with COMT Antibody (AP16308PU-N at 0.3 µg/ml). Primary incubation was 1 hour. Detected by chemiluminescence.
COMT MaxPab polyclonal antibody. Western Blot analysis of COMT expression in human placenta.
Western Blot analysis of COMT expression in transfected 293T cell line (H00001312-T01) by COMT MaxPab polyclonal antibody.Lane 1: COMT transfected lysate(24.31 KDa).Lane 2: Non-transfected lysate.
COMT MaxPab rabbit polyclonal antibody. Western Blot analysis of COMT expression in mouse liver.
Immunoprecipitation of COMT transfected lysate using anti-COMT MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with COMT MaxPab mouse polyclonal antibody (B01) (H00001312-B01).
Human lung microsome; . Lanes: 1. 100 ug Human lung microsome lysate. Primary Antibody Dilution: 1: 1000. Secondary Antibody: . Secondary Antibody Dilution: 1: 10000. Gene Name: COMT. Submitted by: Jing Peng, Fox Chase Cancer Center.; COMT antibody - middle region (ARP44327_P050) in Human lung microsome cells using Western Blot
Human, Mouse, Rat; COMT antibody - middle region (ARP44327_P050) validated by WB using rat liver, mouse lung, human brain at 1:1000.; COMT antibody - middle region (ARP44327_P050) in Human, Mouse, Rat cells using Western Blot
Human Stomach; WB Suggested Anti-COMT Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Stomach; COMT antibody - middle region (ARP44327_P050) in Human Stomach cells using Western Blot
Immunofluorescent analysis of COMT staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of COMT expression in HEK293T (A); Raw264.7 (B); rat liver (C) whole cell lysates.
Staining of human testis lysate (35µg protein in RIPA buffer) with A) TA303130 (0.03µg/ml) and B) TA302983 (1µg/ml). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-COMT antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-COMT antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Western blot analysis of extracts from HepG2 cells and HUVEC cells, using COMT antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-COMT Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Stomach
Lanes: 1. 100 ug Human lung microsome lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Secondary Antibody Dilution: 1: 10000; Gene Name: COMT; Submitted by: Jing Peng, Fox Chase Cancer Center;
COMT antibody - middle region validated by WB using rat liver, mouse lung, human brain at 1: 1000.
Human, Prostate: Formalin-Fixed Parafin-Embedded (FFPE)
Anti-COMT antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-COMT antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-COMT antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-COMT antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-COMT antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-COMT antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-COMT antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-COMT antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-COMT antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP16308PU-N | COMT (N-term) antibody |
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Goat | Aff - Purified | Can, Hu, Ms, Rt | WB |
0.1 mg /
€380.00
|
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| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Western blotting. |
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| TA303130 | COMT antibody |
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| Goat Polyclonal Antibody against COMT (Internal Region) | |||||||
 |
Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA318461 | COMT (N-term) antibody |
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| Anti-COMT Goat Polyclonal Antibody | |||||||
 |
Goat | Can, Hu, Ms, Rt | E, P, WB |
50 µg /
€425.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TA326243 | COMT antibody |
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| Rabbit polyclonal COMT antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA341237 | COMT antibody |
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| Rabbit Polyclonal Anti-COMT Antibody (Internal) | |||||||
 |
Rabbit | Purified | Hu, Mky |
50 µg /
€425.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TA341247 | COMT antibody |
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| Rabbit Polyclonal Anti-COMT Antibody (Internal) | |||||||
 |
Rabbit | Purified | Hu, Mky, Rb |
50 µg /
€425.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TA341303 | COMT (N-term) antibody |
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| Rabbit Polyclonal Anti-COMT Antibody (N-Terminus) | |||||||
|
Rabbit | Purified | Hu | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA341304 | COMT antibody |
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| Rabbit Polyclonal Anti-COMT Antibody (Internal) | |||||||
|
Rabbit | Purified | Hu, Ms, Rt | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA341305 | COMT antibody |
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| Rabbit Polyclonal Anti-COMT Antibody (Internal) | |||||||
|
Rabbit | IgG | Aff - Purified | Hu | P |
50 µg /
€425.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA341306 | COMT (C-term) antibody |
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| Rabbit Polyclonal Anti-COMT Antibody (C-Terminus) | |||||||
|
Rabbit | Purified | Hu | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301275 | COMT (transcript variant 1) |
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| Purified recombinant protein of Homo sapiens catechol-O-methyltransferase (COMT), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP304127 | COMT (transcript variant 4) |
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| Recombinant protein of human catechol-O-methyltransferase (COMT), transcript variant 4 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY400254 | COMT overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR205966L2V | Lenti ORF particles, Comt (GFP-tagged ORF) - Rat catechol-O-methyltransferase (Comt), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR205966L1V | Lenti ORF particles, Comt (Myc-DDK-tagged ORF) - Rat catechol-O-methyltransferase (Comt), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||