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CPSF4 antibody

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Principal name

CPSF4 antibody

Alternative names for CPSF4 antibody

CPSF30, NAR, NEB1, No arches homolog, Cleavage and polyadenylation specificity factor subunit 4, Cleavage and polyadenylation specificity factor 30 kDa subunit, NS1 effector domain-binding protein 1

SwissProt ID

O19137 (Bovin), O95639 (Human), Q5FVR7 (Rat), Q8BQZ5 (Mouse)

Gene ID

10898 (CPSF4), 54188 (Cpsf4), 304277

Ncbi ID

9606, NP_006684, NP_001075028, AAH03101, NM_006693, NM_001081559, NP_001075028.1, NP_848671.1, BC057067, NM_178576, NM_001012351

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of CPSF4 antibody

Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene.

General readings

Jenny,A., et al. (1994) Mol. Cell. Biol. 14 (12), 8183-8190Barabino,S.M., et al. (1997) Nephrol Genes Dev. 11 (13), 1703-1716

H00010898-B01 - CPSF4
Western Blot analysis of CPSF4 expression in transfected 293T cell line (H00010898-T01) by CPSF4 MaxPab polyclonal antibody.Lane 1: CPSF4 transfected lysate(26.95 KDa).Lane 2: Non-transfected lysate.
H00010898-B02 - CPSF4
Western Blot analysis of CPSF4 expression in transfected 293T cell line (H00010898-T02) by CPSF4 MaxPab polyclonal antibody.Lane 1: CPSF4 transfected lysate(26.73 KDa).Lane 2: Non-transfected lysate.
H00010898-B02P - CPSF4
Western Blot analysis of CPSF4 expression in transfected 293T cell line (H00010898-T03) by CPSF4 MaxPab polyclonal antibody.Lane 1: CPSF4 transfected lysate(26.73 KDa).Lane 2: Non-transfected lysate.
ARP40675_P050 - CPSF4
Human Jurkat; WB Suggested Anti-CPSF4 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate. CPSF4 is supported by BioGPS gene expression data to be expressed in Jurkat.; CPSF4 antibody - C-terminal region (ARP40675_P050) in Human Jurkat cells using Western Blot
AP51059PU-N - CPSF4
Western blot analysis of CPSF4 Antibody (Center) Cat.-No AP51059PU-N in Jurkat cell line lysates (35ug/lane).CPSF4 (arrow) was detected using the purified Pab.
AR50847PU-N - CPSF4
TA345981 - CPSF4
WB Suggested Anti-CPSF4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysateCPSF4 is supported by BioGPS gene expression data to be expressed in Jurkat
TA349826 - CPSF4
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Hela cells, 293T cells, Primary antibody: (CPSF4 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
TA349825 - CPSF4
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (CPSF4 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
TA349825 - CPSF4
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (CPSF4 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)

10 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CPSF4 antibody
  Rabbit Polyclonal Anti-CPSF4 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (CPSF4 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

CPSF4 antibody
  Rabbit Polyclonal Anti-CPSF4 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Hela cells, 293T cells, Primary antibody: (CPSF4 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

CPSF4 (Center) antibody
Western blot analysis of CPSF4 Antibody (Center) Cat.-No AP51059PU-N in Jurkat cell line lysates (35ug/lane).CPSF4 (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Lysates

Catalog No.    

CPSF4 overexpression lysate
CPSF4 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

CPSF4 overexpression lysate
CPSF4 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

CPSF4 overexpression lysate
CPSF4 overexpression lysate  
0.1 mg / €315.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Cpsf4 (GFP-tagged) - Mouse cleavage and polyadenylation specific factor 4 (cDNA clone MGC:67409 IMAGE:5689404), 200 uL, >10^7 TU/mL
Lenti ORF particles, Cpsf4 (GFP-tagged) - Mouse cleavage and polyadenylation specific factor 4 (cDNA clone MGC:67409 IMAGE:5689404), 200 uL, >10^7 TU/mL  
200 µl / €840.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Cpsf4 (Myc-DDK-tagged) - Mouse cleavage and polyadenylation specific factor 4 (cDNA clone MGC:67409 IMAGE:5689404), 200 uL, >10^7 TU/mL
Lenti ORF particles, Cpsf4 (Myc-DDK-tagged) - Mouse cleavage and polyadenylation specific factor 4 (cDNA clone MGC:67409 IMAGE:5689404), 200 uL, >10^7 TU/mL  
200 µl / €740.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Cpsf4 (GFP-tagged ORF) - Rat cleavage and polyadenylation specific factor 4 (Cpsf4), 200 uL, >10^7 TU/mL
Lenti ORF particles, Cpsf4 (GFP-tagged ORF) - Rat cleavage and polyadenylation specific factor 4 (Cpsf4), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Cpsf4 (Myc-DDK-tagged ORF) - Rat cleavage and polyadenylation specific factor 4 (Cpsf4), 200 uL, >10^7 TU/mL
Lenti ORF particles, Cpsf4 (Myc-DDK-tagged ORF) - Rat cleavage and polyadenylation specific factor 4 (Cpsf4), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

10 Item(s)

per page

Available Filters

Product Type
  1. Lysate (3)
  2. Mouse Lenti ORF Particle (2)
  3. Primary Antibody (3)
  4. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (3)
Target
  1. CPSF4 (3)
Property
  1. Center (1)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (2)
Applications
  1. Enzyme Immunoassay (E) (2)
  2. Western blot / Immunoblot (WB) (2)
  3. Paraffin Sections (P) (1)
Presentation
  1. Purified (2)
  2. Aff - Purified (1)
Host
  1. Rabbit (3)
Images
  1. 1 image (3)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
  2. IHC (1)
Manufacturer
  1. OriGene Technologies (10)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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