CRABP2 antibody
Principal name
CRABP2 antibody
Alternative names for CRABP2 antibody
CRABP-II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid-binding protein II
Ncbi ID
NP_001869, NP_001186652.1, NP_001186652, NM_001878, 9606, NP_031785.1, NM_007759, NM_017244
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of CRABP2 antibody
The cellular retinoic acid-binding protein (CRABP)-I and a related isoform CRABP-II are nuclear receptors for retinoic acid (RA), an important regulator of cell growth and differentiation in fetal and adult tissues. These CRABP proteins mediate the downstream effects of RA in distinct ways. CRABP-I negatively regulates the activity of RA by enhancing the production of RAmetabolizing enzymes and increasing the rate at which RA is degraded. CRABP-II enhances the effects of RA by directly interacting with RA receptors (RAR) and, in turn, promoting the formation of RAR-RA complexes and stimulating RA-mediated gene transcription. Both CRABP-I and CRABP-II are expressed in the embryo, and CRABP-I is ubiquitously expressed in various adult tissues. The expression of CRABP-II is elevated in cells that synthesize relatively large amounts of RA, and it is also predominantly expressed in skin, uterus, ovary, and in the choroid plexus.
General readings
1. Yao PL et al. Activation of peroxisome proliferator-activated receptor-ß/d (PPAR-ß/d) inhibits human breast cancer cell line tumorigenicity. Mol Cancer Ther 13:1008-17 (2014). Human .
2. Borland MG et al. Stable over-expression of PPARß/d and PPAR? to examine receptor signaling in human HaCaT keratinocytes. Cell Signal 23:2039-50 (2011). WB ; Human .
Immunohistochemistry: AM09062PU-N CRABP2 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Uterus at 10 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot analysis: Cell lysates of mouse eye (60 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP2 antibody (1/250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot (WB) analysis of CRABP-II antibody (Cat.-No.: AP01466PU-N) in extracts from RAW264.7 cell
Western blot analysis of CRABP2 (arrow) using CRABP2 Antibody (C-term) Cat.-No AP51069PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CRABP2 gene.
Western blot analysis of CRABP2 Antibody (C-term) Cat.-No AP51069PU-N MDA-MB453 cell line lysates (35ug/lane). This demonstrates the CRABP2 antibody detected the CRABP2 protein (arrow).
Human PANC1; WB Suggested Anti-CRABP2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: PANC1 cell lysate. CRABP2 is supported by BioGPS gene expression data to be expressed in PANC1.; CRABP2 antibody - middle region (ARP41982_P050) in Human PANC1 cells using Western Blot
Human 721_B; WB Suggested Anti-CRABP2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate; CRABP2 antibody - middle region (ARP41983_P050) in Human 721_B cells using Western Blot
Immunofluorescence of purified MaxPab antibody to CRABP2 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of CRABP2 expression in transfected 293T cell line (H00001382-T01) by CRABP2 MaxPab polyclonal antibody.Lane 1: CRABP2 transfected lysate(15.18 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CRABP2 expression in transfected 293T cell line (H00001382-T01) by CRABP2 MaxPab polyclonal antibody.Lane 1: CRABP2 transfected lysate(15.70 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical analysis of CRABP2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CRABP2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of CRABP2 expression in A431 (A); HeLa (B) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CRABP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800069)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRABP2 (RC200221, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRABP2.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CRABP2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC200221 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CRABP2 antibody(TA800069), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRABP2 (RC200221, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRABP2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CRABP2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CRABP2 mouse monoclonal antibody (UM800069). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
Western blot analysis of extracts from HT-29 cells, using CRABP2 antibody.The lane on the right is treated with the synthesized peptide.
Predicted band size: 16 kDa . Positive control: MCF-7 and HT-29 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 12%SDS-PAGE Lane1,2: MCF-7 and HT-29 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Host: Rabbit; Target Name: CRABP2; Sample Tissue: Hela Whole Cell lysates; Antibody Dilution: 1.0ug/ml
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (CRABP2 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (CRABP2 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
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- Cited (1)
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA800069 | CRABP2 antibody |
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| CRABP2 mouse monoclonal antibody, clone OTI10D6 (formerly 10D6) | |||||||
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Mouse | IgG1 | OTI10D6 | Purified | Hu | F, P, WB |
0.1 ml /
€379.00
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Top Selling product! Highly validated antibody!
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| UM800069 | CRABP2 antibody |
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| CRABP2 mouse monoclonal antibody,clone UMAB176 | |||||||
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Mouse | IgG1 | UMAB176 | Purified | Hu | P |
0.1 ml /
€468.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
Highly validated antibody!
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| UM870069 | CRABP2 antibody |
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| CRABP2 mouse monoclonal antibody,clone UMAB176 | |||||||
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Mouse | IgG1 | UMAB176 | Purified | Hu | P |
30 µl /
€180.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
Highly validated antibody!
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| TA313145 | CRABP2 antibody |
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| Rabbit polyclonal anti-CRABP2 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA324128 | CRABP2 antibody |
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| Anti-CRABP2 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Rt | WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA349827 | CRABP2 antibody |
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| Rabbit Polyclonal Anti-CRABP2 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA800027 | CRABP2 antibody |
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| CRABP2 mouse monoclonal antibody, clone OTI3C11 (formerly 3C11) | |||||||
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Mouse | IgG1 | OTI3C11 | Purified | Hu | WB |
0.1 ml /
€360.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AM09062PU-N | CRABP2 antibody |
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Mouse | IgG2a | AT2E11 | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€330.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AM09062PU-S | CRABP2 antibody |
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Mouse | IgG2a | AT2E11 | Purified | Hu, Ms | E, ICC/IF, P, WB |
50 µl /
€220.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP01466PU-N | CRABP2 antibody |
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Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€370.00
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| OriGene Technologies GmbH | |||||||
| AP51069PU-N | CRABP2 (C-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300221 | CRABP2 |
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| Recombinant protein of human cellular retinoic acid binding protein 2 (CRABP2) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419677 | CRABP2 overexpression lysate |
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0.1 mg /
€295.00
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| OriGene Technologies, Inc. | ||
Published recently! |
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Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR200863L2V | Lenti ORF particles, Crabp2 (GFP-tagged) - Mouse cellular retinoic acid binding protein II (Crabp2), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
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| OriGene Technologies, Inc. | ||
| MR200863L1V | Lenti ORF particles, Crabp2 (Myc-DDK-tagged) - Mouse cellular retinoic acid binding protein II (Crabp2), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
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| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR215086L2V | Lenti ORF particles, Crabp2 (GFP-tagged ORF) - Rat cellular retinoic acid binding protein 2 (Crabp2), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
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| OriGene Technologies, Inc. | ||
| RR215086L1V | Lenti ORF particles, Crabp2 (Myc-DDK-tagged ORF) - Rat cellular retinoic acid binding protein 2 (Crabp2), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
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| OriGene Technologies, Inc. | ||