CRKL antibody
Principal name
CRKL antibody
Alternative names for CRKL antibody
Crk-like protein, v-crk avian sarcoma virus CT10 oncogene homolog-like
Ncbi ID
NP_005198, NP_005198.1, 9606, NM_005207, NP_031790.2, NM_007764
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of CRKL antibody
SH2 and SH3 (Src homology) domains were originally identified as critical functional domains within non-receptor proteins with tyrosine kinase activity. A subset of these proteins appears to exist predominately of SH2/SH3 domains in the absence of detectable catalytic domains. One of the first members of the family to be identified, Crk, is a transformation-specific protein that induces elevation of cellular phosphotyrosine levels, but lacks tyrosine kinase activity itself. A second protein, Nck, consists solely of three SH3 domains and one SH2 domain, while GRB2 contains an SH2 domain flanked on both sides by SH3 domains. A member of this protein class, Crk-L, is encoded by a gene located on chromosome 22, band 11, centromeric of the chronic myelogenous leukemia breakpoint region. Crk-L encodes a 303 amino acid protein with one SH2 and two SH3 domains, and a predicted molecular weight of 36 kDa.
General readings
Zhang,W., et al., (2003) J. Biol. Chem. 278 (26), 23978-23983
Human K562; WB Suggested Anti-CRKL Antibody Titration: 0.2-1 ug/ml. Positive Control: K562 cell lysate. CRKL is strongly supported by BioGPS gene expression data to be expressed in Human K562 cells.; CRKL antibody - middle region (AVARP01043_P050) in Human K562 cells using Western Blot
Proximity Ligation Analysis (PLA) of protein-protein interactions between HCK and CRKL Mahlavu cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-CRKL mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Proximity Ligation Analysis (PLA) of protein-protein interactions between PTK2 and CRKL. Huh7 cells were stained with anti-PTK2 rabbit purified polyclonal 1:600 and anti-CRKL mouse monoclonal antibody 1:100. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Proximity Ligation Analysis (PLA) of protein-protein interactions between GAB1 and CRKL. HeLa cells were stained with anti-GAB1 rabbit purified polyclonal 1:1200 and anti-CRKL mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of CRKL expression in transfected 293T cell line by CRKL monoclonal antibody (M03), clone 4B5.Lane 1: CRKL transfected lysate(33.8 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of CRKL over-expressed 293 cell line, cotransfected with CRKL Validated Chimera RNAi ( Cat # H00001399-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CRKL monoclonal antibody (M03), clone 4B5 (Cat # H00001399-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Detection limit for recombinant GST tagged CRKL is approximately 0.3ng/ml as a capture antibody.
Immunofluorescence of purified MaxPab antibody to CRKL on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of CRKL expression in transfected 293T cell line (H00001399-T01) by CRKL MaxPab polyclonal antibody.Lane 1: CRKL transfected lysate(33.33 KDa).Lane 2: Non-transfected lysate.
CRKL polyclonal antibody ( Cat # PAB6084 ) staining ( 0.01 µg/ml ) of K-562 lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Immunofluorescence of purified MaxPab antibody to CRKL on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of CRKL expression in transfected 293T cell line (H00001399-T01) by CRKL MaxPab polyclonal antibody.Lane 1: CRKL transfected lysate(33.33 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Analysis (PLA) of protein-protein interactions between CRKL and PTPN11. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal 1:600 and anti-PTPN11 mouse purified polyclonal antibody 1:100. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Proximity Ligation Analysis (PLA) of protein-protein interactions between CRKL and SOS1. Huh7 cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-SOS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Proximity Ligation Analysis (PLA) of protein-protein interactions between CRKL and EGFR. HeLa cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-EGFR mouse purified polyclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of CRKL expression in transfected 293T cell line (H00001399-T01) by CRKL MaxPab polyclonal antibody.Lane 1: CRKL transfected lysate(33.80 KDa).Lane 2: Non-transfected lysate.
CRKL MaxPab rabbit polyclonal antibody. Western Blot analysis of CRKL expression in A-431.
Immunohistochemistry analyzes of CrkL antibody (AP20279PU-N) in paraffin-embedded human lung carcinoma tissue.
Western blot analysis of CrkL antibody (AP20279PU-N) in extracts from COS7 cells.
Immunohistochemistry (IHC) analyzes of p-CrkL (Try207) antibody (Cat.-No.: AP20935PU-N) in paraffin-embedded human lung adenocarcinoma tissue.
Immunohistochemistry (IHC) analyzes of CrkL antibody (Cat.-No.: AP21023PU-N) in paraffin-embedded human lung adenocarcinoma tissue.
Recombinant human CRKL, 1-303 aa, His-tagged
Western blot analysis using CRKL mAb against human CRKL (AA: 100-303) recombinant protein. (Expected MW is 60 kDa)
Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)
Flow cytometric analysis of NIH/3T3 cells using CRKL mouse mAb (blue) and negative control (red).
TA302748 (0.01µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
WB Suggested Anti-CRKL Antibody Titration: 0.2-1 ug/ml; Positive Control: K562 cell lysate CRKL is strongly supported by BioGPS gene expression data to be expressed in Human K562 cells
Gel: 15%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Lovo cells, Raji cells, A172 cells, Primary antibody: (CRKL Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (CRKL Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using (CRKL Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
WB Suggested Anti-CRKL Antibody; Titration: 1.0 ug/ml; Positive Control: K562 Whole CellCRKL is strongly supported by BioGPS gene expression data to be expressed in Human K562 cells
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302748 | CRKL (C-term) antibody |
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| Goat Polyclonal Antibody against CRKL | |||||||
 |
Goat | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA351105 | CRKL antibody |
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| Rabbit Polyclonal Anti-CRKL Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| AM06643SU-N | CRKL antibody |
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 |
Mouse | IgG1 | 5E12 | Ascites | Hu | E, WB |
0.1 ml /
€410.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AP20279PU-N | CRKL antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP20935PU-N | CRKL pTyr207 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| AP21023PU-N | CRKL antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| SP1398P | CRKL antibody |
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 |
Goat | Purified | Hu, Ms | WB |
0.1 mg /
€740.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308129 | CRKL |
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| Recombinant protein of human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY417444 | CRKL overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |