CROT antibody
Principal name
CROT antibody
Alternative names for CROT antibody
COT, Peroxisomal carnitine O-octanoyltransferase
Ncbi ID
9606, NP_066974, NP_114193.1, NM_001143935, NP_066974.2, NP_076222.1, NM_023733, NM_031987, NM_021151
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F)
Background of CROT antibody
- Kit Component:
- - KN303846G1, Crot gRNA vector 1 in pCas-Guide vector
- KN303846G2, Crot gRNA vector 2 in pCas-Guide vector
- KN303846D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Morillas,M., (2002) J. Biol. Chem. 277 (13), 11473-11480
CROT monoclonal antibody (M01), clone 1A6. Western Blot analysis of CROT expression in NIH/3T3 ( Cat # L018V1 ).
CROT monoclonal antibody (M01), clone 1A6. Western Blot analysis of CROT expression in Raw 264.7 ( Cat # L024V1 ).
CROT monoclonal antibody (M01), clone 1A6. Western Blot analysis of CROT expression in PC-12 ( Cat # L012V1 ).
CROT monoclonal antibody (M01), clone 1A6 Western Blot analysis of CROT expression in HeLa ( Cat # L013V1 ).
CROT polyclonal antibody (A01), Lot # 051205JC01 Western Blot analysis of CROT expression in MES-SA/Dx5 ( Cat # L021V1 ).
Western Blot analysis of CROT expression in transfected 293T cell line (H00054677-T01) by CROT MaxPab polyclonal antibody.Lane 1: CROT transfected lysate(67.32 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CROT expression in transfected 293T cell line (H00054677-T02) by CROT MaxPab polyclonal antibody.Lane 1: CROT transfected lysate(70.20 KDa).Lane 2: Non-transfected lysate.
Human HepG2; WB Suggested Anti-CROT Antibody Titration: 2.5ug/ml. Positive Control: HepG2 cell lysate; CROT antibody - N-terminal region (ARP48873_T100) in Human HepG2 cells using Western Blot
Formalin-fixed and paraffin-embedded human brain tissue reacted with CROT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of CROT Antibody (N-term) (Cat. #AP51088PU-N) in K562 cell line and mouse liver tissue lysates (35µg/lane). CROT (arrow) was detected using the purified Pab.
CROT Antibody (N-term)(Cat. #AP51088PU-N) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Staining of Mouse Liver lysate using CROT Antibpdy Cat.-No AP32804PU-N at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Sample (50 ug of whole cell lysate). A: mouse liver. 7.5% SDS PAGE. TA307959 diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: 293T. B: A431 . 7.5% SDS PAGE. COT antibody. TA307959 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using CROT(TA307959) antibody at 1:100 dilution.
(0.3ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: 293T cells, mouse kidney tissue, Primary antibody: (CROT Antibody) at dilution 1/270, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (CROT Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (CROT Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
WB Suggested Anti-CROT Antibody Titration: 2.5 ug/ml; Positive Control: HepG2 cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP32804PU-N | CROT (178-199) antibody |
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Goat | Aff - Purified | Can, Ms, Rt | E, WB |
0.1 mg /
€380.00
|
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| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Western blotting. |
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| TA307959 | CROT antibody |
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| Rabbit Polyclonal antibody to CROT (carnitine O-octanoyltransferase) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA311543 | CROT (C-term) antibody |
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| Goat Anti-Crot (rat) Antibody | |||||||
 |
Goat | Aff - Purified | Rt | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA349832 | CROT antibody |
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| Rabbit Polyclonal Anti-CROT Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| AP51088PU-N | CROT (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu, Ms | F, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307888 | CROT (transcript variant 2) |
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| Recombinant protein of human carnitine O-octanoyltransferase (CROT), transcript variant 2 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY428407 | CROT overexpression lysate |
|
 |
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR209410L1V | Lenti ORF particles, Crot (Myc-DDK-tagged) - Mouse carnitine O-octanoyltransferase (Crot), 200 uL, >10^7 TU/mL |
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|
200 µl /
€1,210.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR201019L2V | Lenti ORF particles, Crot (GFP-tagged ORF) - Rat carnitine O-octanoyltransferase (Crot), 200 uL, >10^7 TU/mL |
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|
200 µl /
€1,350.00
|
|
| OriGene Technologies, Inc. | ||
| RR201019L1V | Lenti ORF particles, Crot (Myc-DDK-tagged ORF) - Rat carnitine O-octanoyltransferase (Crot), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,210.00
|
|
| OriGene Technologies, Inc. | ||