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Cryptochrome-2 antibody

Principal name

Cryptochrome-2 antibody

Alternative names for Cryptochrome-2 antibody

CRY2, KIAA0658

SwissProt ID

Q49AN0 (Human), Q8QG60 (Chick), Q923I8 (Rat), Q9R194 (Mouse)

Gene ID

1408 (CRY2), 12953 (Cry2), 170917

Ncbi ID

NP_001120929.1, 9606, NP_001120929, NP_066940, NP_001106804.1, BC054794, BC066799, NM_009963, NM_001113333, NM_133405, NM_001127457

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Rt (Rat), Sh (Sheep), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of Cryptochrome-2 antibody

Various biochemical, physiological and behavioural processes display circadian rhythms controlled by an internal biological clock. The central gears driving this clock appear to be composed of an autoregulatory transcription/posttranslation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.

Figure 1. Staining Cryptochrome 2 (CRY2) in Skeletal Muscle by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.

AP07904PU-N

Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with CRY2 polyclonal antibody ( Cat # PAB1976 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

PAB1976

The CRY2 polyclonal antibody ( Cat # PAB1976 ) is used in Western blot to detect CRY2 in mouse liver tissue lysate.

PAB1976

Detection limit for recombinant GST tagged CRY2 is approximately 1ng/ml as a capture antibody.

H00001408-M03

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

AP13098PU-N

The anti-Cry2 C-term Pab is used in Western blot to detect Cry2 in mouse liver tissue lysate.

AP13098PU-N

Immunofluorescence of purified MaxPab antibody to CRY2 on HeLa cell. [antibody concentration 10 ug/ml]

H00001408-B01P

Western Blot analysis of CRY2 expression in transfected 293T cell line (H00001408-T02) by CRY2 MaxPab polyclonal antibody.Lane 1: CRY2 transfected lysate(65.23 KDa).Lane 2: Non-transfected lysate.

H00001408-B01P

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502905)

TA502905

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502905)

TA502905

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502905)

TA502905

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502905)

TA502905

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502905)

TA502905

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502905)

TA502905

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502905)

TA502905

Anti-CRY2 mouse monoclonal antibody (TA502905) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRY2(RC207337 ).

TA502905

Immunofluorescent staining of HeLa cells using anti-CRY2 mouse monoclonal antibody (TA502905).

TA502905

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRY2 (RC207337 , Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRY2.

TA502905

Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-CRY2 monoclonal antibody.(1:200)

TA502905

Flow cytometric Analysis of Hela cells, using anti-CRY2 antibody(TA502905),(Red), compared to a nonspecific negative control antibody,(Blue).

TA502905

Flow cytometric Analysis of Jurkat cells, using anti-CRY2 antibody(TA502905),(Red), compared to a nonspecific negative control antibody,(Blue).

TA502905

TP307337

WB Suggested Anti-CRY2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: U937 cell lysate

TA340193

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA502905	Cryptochrome-2 antibody
	CRY2 mouse monoclonal antibody, clone OTI1H5 (formerly 1H5)
	Mouse	IgG2a	OTI1H5	Purified	Hu	F, ICC/IF, P, WB	0.1 ml / €379.00
							OriGene Technologies, Inc.
+12 additional images	Highly validated antibody!
AP07904PU-N	Cryptochrome-2 (579-593) antibody
	Rabbit			Purified	Hu	E, P	50 µg / €425.00
							OriGene Technologies GmbH
AP13098PU-N	Cryptochrome-2 (C-term) antibody
	Rabbit	Ig		Purified	Hu, Ms	P, WB	0.4 ml / €415.00
							OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP307337	Cryptochrome-2 (transcript variant 1)
	Recombinant protein of human cryptochrome 2 (photolyase-like) (CRY2), transcript variant 1
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR209229L2V	Lenti ORF particles, Cry2 (GFP-tagged) - Mouse cryptochrome 2 (photolyase-like) (cDNA clone MGC:65642 IMAGE:6834104), 200 uL, >10^7 TU/mL
		200 µl / €1,240.00
		OriGene Technologies, Inc.

MR209230L2V	Lenti ORF particles, Cry2 (GFP-tagged) - Mouse cryptochrome 2 (photolyase-like) (cDNA clone MGC:76472 IMAGE:30103169), 200 uL, >10^7 TU/mL
		200 µl / €1,240.00
		OriGene Technologies, Inc.

MR209229L1V	Lenti ORF particles, Cry2 (Myc-DDK-tagged) - Mouse cryptochrome 2 (photolyase-like) (cDNA clone MGC:65642 IMAGE:6834104), 200 uL, >10^7 TU/mL
		200 µl / €1,110.00
		OriGene Technologies, Inc.

MR209230L1V	Lenti ORF particles, Cry2 (Myc-DDK-tagged) - Mouse cryptochrome 2 (photolyase-like) (cDNA clone MGC:76472 IMAGE:30103169), 200 uL, >10^7 TU/mL
		200 µl / €1,110.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR210144L2V	Lenti ORF particles, Cry2 (GFP-tagged ORF) - Rat cryptochrome 2 (photolyase-like) (Cry2), 200 uL, >10^7 TU/mL
		200 µl / €1,320.00
		OriGene Technologies, Inc.

RR210144L1V	Lenti ORF particles, Cry2 (Myc-DDK-tagged ORF) - Rat cryptochrome 2 (photolyase-like) (Cry2), 200 uL, >10^7 TU/mL
		200 µl / €1,180.00
		OriGene Technologies, Inc.

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