CUGBP1 antibody
Principal name
CUGBP1 antibody
Alternative names for CUGBP1 antibody
CELF-1, BRUNOL2, CELF1, CUGBP, NAB50, Bruno-like protein 2, CUG-BP- and ETR-3-like factor 1,RNA-binding protein BRUNOL-2, CUG triplet repeat RNA-binding protein 1, Deadenylation factor CUG-BP, 50 kDa nuclear polyadenylated RNA-binding protein, Embryo deadenylation element-binding protein homolog
SwissProt ID
P28659 (Mouse), Q28HE9 (Xentr), Q4QQT3 (Rat), Q5F3T7 (Chick), Q8R532 (Mouse), Q92879 (Human)
Ncbi ID
NP_001020767, NP_001020767.1, 9606, NM_006560, NM_198700, NM_001172640, NP_941989, NP_006551, NM_001025596, NM_001172639, NP_059064.2, NM_198683, NM_017368, NM_001025421
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of CUGBP1 antibody
Myotonic dystrophy (DM) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase gene (DMPK). CUG-BP1 and CUG-BP2 are proteins that bind specifically to (CUG)8 oligonucleotides in vitro. While CUG-BP1 has the major binding activity in normal cells, nuclear CUG-BP2 binding activity increases in DM cells. Both CUG-BP1 and CUG-BP2 are isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. CUG-BP1, an RNA CUG triplet repeat binding protein, regulates splicing and translation of various RNAs. Expansion of RNA CUG repeats in the DMPK in DM is associated with alterations in binding activity of CUG-BP1 as well as alterations in the translation of the C/EBPbeta transcription factor. CUG-BP1 is an important regulator of initiation from different AUG codons of C/EBPbeta mRNA. In normal cells, CUG-BP1 up-regulates the p21 protein during differentiation by inducing the translation of p21 via binding to a GC-rich sequence located within the 5' region of p21 mRNA. In DM cells, failure to accumulate CUG-BP1 leads to a reduction of p21 and alterations in other proteins responsible for cell cycle withdrawal.
Immunohistochemistry (IHC) analyzes of CUG-BP1/2 antibody (Cat.-No.: AP20451PU-N) in paraffin-embedded human brain tissue.
Western blot (WB) analysis of CUG-BP1/2 antibody (Cat.-No.: AP20451PU-N) in extracts from HeLa cells.
Human Liver; Liver; CUGBP1 antibody - middle region (ARP40327_P050) in Human Liver cells using Immunohistochemistry
Western Blot analysis of CUGBP1 expression in transfected 293T cell line (H00010658-T01) by CUGBP1 MaxPab polyclonal antibody.Lane 1: CUGBP1 transfected lysate(53.13 KDa).Lane 2: Non-transfected lysate.
CUGBP1 MaxPab rabbit polyclonal antibody. Western Blot analysis of CUGBP1 expression in mouse liver.
Western Blot analysis of CUGBP1 expression in transfected 293T cell line (H00010658-T02) by CUGBP1 MaxPab polyclonal antibody.Lane 1: CUGBP1 transfected lysate(51.60 KDa).Lane 2: Non-transfected lysate.
Detection of the subcellular distribution of CUGBP1 ( nuclear, non-nucleolar ) in normal and DM1 ( dystrophia myotonica ) myoblasts using CUGBP1 monoclonal antibody, clone 3B1 ( Cat #MAB2351 ).
Western Blot Analysis detection of CUGBP1 in several cell lysates using CUGBP1 monoclonal antibody, clone 3B1 ( Cat #MAB2351 ).
Immunohistochemical analysis of CUGBP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CUGBP1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of CUGBP1 expression in HL60 (A); HeLa (B); NIH3T3 (C); Sol8 (D) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500951, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500951, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500951, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500951, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500951, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500951, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500951, Dilution 1:50)
Anti-CUGBP1 mouse monoclonal antibody (TA500951) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CUGBP1(RC206275).
Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-CUGBP1 monoclonal antibody.(1:200)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CUGBP1 (RC206275, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CUGBP1.
Detection of CUG-BP1 in several cell lysates
Detection of the subcellular distribution of CUGBP1 (nuclear, non-nucleolar) in normal and DM1 (dystrophia myotonica) myoblasts
Western blot analysis of extracts from HeLa cells and HepG2 cells, using CELF-1 antibody.The lane on the right is treated with the synthesized peptide.
Liver
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- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500951 | CUGBP1 antibody |
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| anti-CUGBP1 mouse monoclonal antibody, clone OTI5B8 (formerly 5B8) | |||||||
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Mouse | IgG2b | OTI5B8 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +9 additional images |
Highly validated antibody!
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| TA301501 | CUGBP1 antibody |
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| Mouse Monoclonal Antibody against CUG-BP1 (3B1) | |||||||
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Mouse | IgG1 | 3B1 | Purified | Bov, Hu, Ms, Por, Rb, Rt | WB |
0.1 ml /
€475.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA312319 | CUGBP1 antibody |
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| Rabbit polyclonal anti-CELF-1 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| AP20451PU-N | CUGBP1 (+CUGBP2) antibody |
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Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€370.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP306275 | CUGBP1 (transcript variant 1) |
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| Recombinant protein of human CUG triplet repeat, RNA binding protein 1 (CUGBP1), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP321943 | CUGBP1 (transcript variant 2) |
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| Recombinant protein of human CUG triplet repeat, RNA binding protein 1 (CUGBP1), transcript variant 2 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401964 | CELF1 overexpression lysate |
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0.1 mg /
€295.00
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| OriGene Technologies, Inc. | ||
| LY404819 | CELF1 overexpression lysate |
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0.1 mg /
€495.00
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| OriGene Technologies, Inc. | ||
| LY422453 | CELF1 overexpression lysate |
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0.1 mg /
€495.00
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| OriGene Technologies, Inc. | ||
| LY433163 | CELF1 overexpression lysate |
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0.1 mg /
€315.00
|
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| OriGene Technologies, Inc. | ||
| LY433208 | CELF1 overexpression lysate |
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0.1 mg /
€315.00
|
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| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR220707L2V | Lenti ORF particles, Celf1 (GFP-tagged) - Mouse CUGBP, Elav-like family member 1 (Celf1), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€1,210.00
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| OriGene Technologies, Inc. | ||
| MR220691L2V | Lenti ORF particles, Celf1 (GFP-tagged) - Mouse CUGBP, Elav-like family member 1 (Celf1), transcript variant 2, 200 uL, >10^7 TU/mL |
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200 µl /
€1,170.00
|
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| OriGene Technologies, Inc. | ||
| MR220707L1V | Lenti ORF particles, Celf1 (Myc-DDK-tagged) - Mouse CUGBP, Elav-like family member 1 (Celf1), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€1,080.00
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| OriGene Technologies, Inc. | ||
| MR220691L1V | Lenti ORF particles, Celf1 (Myc-DDK-tagged) - Mouse CUGBP, Elav-like family member 1 (Celf1), transcript variant 2, 200 uL, >10^7 TU/mL |
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200 µl /
€1,040.00
|
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| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR204942L2V | Lenti ORF particles, Celf1 (GFP-tagged ORF) - Rat CUG triplet repeat, RNA binding protein 1 (Cugbp1), 200 uL, >10^7 TU/mL |
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200 µl /
€1,170.00
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| OriGene Technologies, Inc. | ||
| RR204942L1V | Lenti ORF particles, Celf1 (Myc-DDK-tagged ORF) - Rat CUG triplet repeat, RNA binding protein 1 (Cugbp1), 200 uL, >10^7 TU/mL |
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200 µl /
€1,050.00
|
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| OriGene Technologies, Inc. | ||