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Cullin-2 antibody

Principal name

Cullin-2 antibody

Alternative names for Cullin-2 antibody

CUL-2, CUL2

SwissProt ID

Q13617 (Human), Q9D4H8 (Mouse)

Gene ID

8453 (CUL2), 71745 (Cul2)

Ncbi ID

9606, NP_003582, NP_001185706.1, NM_003591, NP_003582.2, NP_083678.2, NM_029402, NM_001198778

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), African clawed frog, Chk (Chicken), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)

Background of Cullin-2 antibody

Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF(Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1 mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3 facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin- like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, or vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.

Immunohistochemical (IHC) analysis in paraffin-embedded human lung carcinoma tissue using Cullin-2 antibody (Cat-No. AP06078PU-N).

AP06078PU-N

Western blot (WB) analysis of CUL-2 antibody (Cat.-No.: AP06078PU-N) at 1/500 dilution
Lane1:MCF-7 cell lysate
Lane2:Mouse liver tissue lysate
Lane3:Rat kidney tissue lysate

AP06078PU-N

Prostate: Formalin-Fixed Paraffin-Embedded (FFPE)

AP07572SU-N

AP15508PU Cullin-2 antibody staining of Human Tonsil.

Immunohistochemical staining with CUL2 polyclonal antibody ( Cat # PAB10036 ) was diluted 1 : 500 to detect CUL2 in human prostate tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

PAB10036

Immunohistochemical staining of CUL2 on formalin fixed, paraffin embedded human tonsil with CUL2 polyclonal antibody.

PAB11632

Human Jurkat; WB Suggested Anti-CUL2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. CUL2 is supported by BioGPS gene expression data to be expressed in Jurkat.; CUL2 antibody - middle region (AVARP03036_P050) in Human Jurkat cells using Western Blot

AVARP03036_P050

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Cullin 2 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of NIH-3T3 cells, using Cullin 2 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from LOVO cells, using Cullin 2 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemical analysis of Cullin 2 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Cullin 2 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of Cullin 2 expression in MCF7 (A); mouse liver (B); rat kidney (C); NIH3T3 (D) whole cell lysates.

TP303304

(1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA311273

Western blot analysis of extracts from LOVO cells, using Cullin 2 antibody (#TA314751).The lane on the right is treated with the synthesized peptide.

TA314751

Western blot analysis of extracts from HepG2 cells (Lane 2), using Cullin 2 Antibody. The lane on the left is treated with systhesized peptide.

TA314751

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cullin 2 antibody (#TA314751).The picture on the right is treated with the synthesized peptide.

TA314751

Immunofluorescence analysis of NIH/3T3 cells, using Cullin 2 antibody (#TA314751).The picture on the right is treated with the synthesized peptide.

TA314751

Anti-CUL2 antibody was diluted 1:500 to detect CUL2 in human prostate tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

TA319397

Western blot analysis of PC3 cell using TA322839(CUL2 Antibody) at dilution 1/440

TA322839

Western blot analysis of extracts of various cell lines, using CUL2 antibody.

TA332601

Immunofluorescence analysis of MCF-7 cell using CUL2 antibody. Blue: DAPI for nuclear staining.

TA332601

Western blot analysis on LOVO cell lysate using Cullin 2 Antibody

TA347363

Human Tonsil stained with anti-Cullin-2 (CUL-2) antibody.

TA353768

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA314751	Cullin-2 antibody
	Rabbit polyclonal anti-Cullin 2 antibody
	Rabbit	IgG	Purified	Hu, Ms	E, ICC/IF, P, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
+3 additional images	Highly validated antibody!
TA311273	Cullin-2 (C-term) antibody
	Goat Anti-CUL2 Antibody
	Goat		Aff - Purified	Bov, Can, Hu, Ms, Rt	E, WB	0.1 mg / €360.00
						OriGene Technologies, Inc.
TA319397	Cullin-2 (C-term) antibody
	Rabbit polyclonal anti-Cul2 antibody
	Rabbit		Serum	Hu	E, IP, WB	0.1 ml / €515.00
						OriGene Technologies, Inc.
TA322839	Cullin-2 antibody
	Anti-CUL2 Rabbit Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
AP07572SU-N	Cullin-2 antibody
	Rabbit			Hu	E, IP, P, WB	50 µl / €425.00
						OriGene Technologies GmbH

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