Cullin-3 antibody
Principal name
Cullin-3 antibody
Alternative names for Cullin-3 antibody
CUL-3, CUL3, KIAA0617
Ncbi ID
9606, NP_003581.1, NM_003590, NP_003581, NP_057925.1, NM_016716
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P)
Background of Cullin-3 antibody
Cullin-3(Cul 3), is a protein that in humans is encoded by the CUL3 gene. Kipreos et al. (1996) identified a conserved gene family, designated cullins, with at least 5 members in nematodes, 6 in humans, and 3 in S. cerevisiae. Human CUL3 is an ortholog of nematode cul3. Hartz (2012) mapped the CUL3 gene to chromosome 2q36.2 based on an alignment of the CUL3 sequence with the genomic sequence (GRCh37). Sumara et al. (2007) found that KLHL9, KLHL13, and CUL3 interacted directly in a 370-kD protein complex in HeLa cell lysates. Rondou et al. (2008) showed that interaction between KLHL12 and the CUL3 ubiquitin ligase complex directed ubiquitination of dopamine receptor D4 (DRD4). KLHL12 interacted directly with CUL3 and with the polymorphic intracellular loop-3 of D4. By immunoprecipitation analysis of HeLa cells and HCT116 human colon carcinoma cells expressing epitope-tagged proteins, Kigoshi et al. (2011) showed that KLHL7 interacted with CUL3 and ROC1 (RBX1).
Breast: Formalin-Fixed Paraffin-Embedded (FFPE)
Immunohistochemical staining with CUL3 polyclonal antibody ( Cat # PAB10037 ) was diluted 1 : 500 to detect CUL3 in human breast tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Human Testis; Immunohistochemistry with Testis tissue at an antibody concentration of 10ug/ml using anti-CUL3 antibody (ARP61264_P050); CUL3 antibody - C-terminal region (ARP61264_P050) in Human Testis cells using Immunohistochemistry
Human Fetal liver; WB Suggested Anti-CUL3 Antibody Titration: 1 ug/ml. Positive Control: Fetal liver cell lysate; CUL3 antibody - C-terminal region (ARP61264_P050) in Human Fetal liver cells using Western Blot
Immunohistochemical analysis of Cullin 3 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of Cullin 3 expression in MDAMB231 (A); MDAMB468 (B); NIH3T3 (C); PC12 (D) whole cell lysates.
Cullin 3 antibody detects Cullin 3 protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human CUL3-transfected 293T cells. 7.5 % SDS-PAGE. Cullin 3 antibody (TA308790) dilution: 1:5000
Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using Cullin 3 antibody.The picture on the right is treated with the synthesized peptide.
Anti-CUL3 antibody was diluted 1:500 to detect CUL3 in human breast tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Western blot analysis of extracts from HepG2 cells, treated with Na2VO3 (0.3nM, 40mins), using ITGB4 (Phospho-Tyr1510) antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cell using CUL3 antibody. Blue: DAPI for nuclear staining.
TA329091 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
WB Suggested Anti-CUL3 Antibody Titration: 1 ug/ml; Positive Control: Fetal liver cell lysate
Immunohistochemistry with Testis tissue at an antibody concentration of 10 ug/ml using anti-CUL3 antibody
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: 293T cells, Primary antibody: (CUL3 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (CUL3 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (CUL3 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308790 | Cullin-3 antibody |
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| Rabbit polyclonal antibody to CUL-3 (cullin 3) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | WB |
0.1 ml /
€345.00
|
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| OriGene Technologies, Inc. | |||||||
| TA312110 | Cullin-3 antibody |
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| Rabbit polyclonal anti-Cullin 3 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA319171 | Cullin-3 (N-term) antibody |
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| Rabbit polyclonal anti-Cul3 antibody | |||||||
 |
Rabbit | Serum | Hu, Ms | E, IP, P, WB |
0.1 ml /
€515.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA326917 | Cullin-3 antibody |
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| Rabbit anti-CUL3 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA329091 | Cullin-3 antibody |
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| Cullin 3 Goat Polyclonal Antibody | |||||||
 |
Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA349289 | Cullin-3 antibody |
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| Rabbit Polyclonal Anti-CUL3 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| AP07573SU-N | Cullin-3 antibody |
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 |
Rabbit | Hu | E, IP, P, WB |
50 µl /
€425.00
|
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| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308066 | Cullin-3 |
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| Recombinant protein of human cullin 3 (CUL3) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR210550L2V | Lenti ORF particles, Cul3 (GFP-tagged) - Mouse cullin 3 (Cul3), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,560.00
|
|
| OriGene Technologies, Inc. | ||
| MR210550L1V | Lenti ORF particles, Cul3 (Myc-DDK-tagged) - Mouse cullin 3 (Cul3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,400.00
|
|
| OriGene Technologies, Inc. | ||
