Cullin-4A antibody
Principal name
Cullin-4A antibody
Alternative names for Cullin-4A antibody
CUL-4A, CUL4A
Ncbi ID
9606, NP_001008895.1, NM_001008895, NM_003589, NP_003580, NP_666319.2, NM_146207
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of Cullin-4A antibody
CUL4a is a core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitiantion of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9.
Western Blot analysis of CUL4A expression in transfected 293T cell line by CUL4A polyclonal antibody (A01).Lane1:CUL4A transfected lysate(87.68 KDa).Lane2:Non-transfected lysate.
Testis: Formalin-Fixed Paraffin-Embedded (FFPE)
Western blot analysis of CUL4A ( arrow ) using rabbit CUL4A polyclonal antibody ( Cat # PAB2988 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the CUL4A gene ( Lane 2 ) ( Origene Technologies ) .
CUL4A MaxPab rabbit polyclonal antibody. Western Blot analysis of CUL4A expression in mouse lung.
CUL4A MaxPab rabbit polyclonal antibody. Western Blot analysis of CUL4A expression in human placenta.
Western Blot analysis of CUL4A expression in transfected 293T cell line (H00008451-T01) by CUL4A MaxPab polyclonal antibody.Lane 1: CUL4A transfected lysate(87.70 KDa).Lane 2: Non-transfected lysate.
CUL4A MaxPab rabbit polyclonal antibody. Western Blot analysis of CUL4A expression in HeLa.
Immunohistochemical staining with CUL4A polyclonal antibody ( Cat # PAB10038 ) was diluted 1 : 500 to detect CUL4 in human testis tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Human Fetal heart; WB Suggested Anti-CUL4A Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Heart; CUL4A antibody - middle region (ARP61665_P050) in Human Fetal heart cells using Western Blot
Hum. Fetal Liver; Host:Rabbit. Target Name:CUL4A. Sample Tissue:Human Fetal Liver. Antibody Dilution: 1.0ug/ml.; CUL4A antibody - middle region (ARP61665_P050) in Hum. Fetal Liver cells using Western Blot
Hum. Fetal Lung; Host:Rabbit. Target Name:CUL4A. Sample Tissue:Human Fetal Lung. Antibody Dilution: 1.0ug/ml.; CUL4A antibody - middle region (ARP61665_P050) in Hum. Fetal Lung cells using Western Blot
Western blot analysis of CUL4a (arrow) using rabbit polyclonal CUL4a Antibody (Human N-term) (Cat.#TA302188).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CUL4a gene (Lane 2) (Origene Technologies).
Immunohistochemistry. anti-CUL4 antibody was diluted 1:500 to detect CUL4 in human testes tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Western blot analysis of Human fetal muscle and fetal brain tissue, hela cell using TA321259(CUL4A Antibody) at dilution 1/325
Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA321259(CUL4A Antibody)(left image) at dilution 1/62, the image on the right is treated with the synthetic peptide.(Original magnification: x200)
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA321259(CUL4A Antibody)(left image) at dilution 1/62, the image on the right is treated with the synthetic peptide.(Original magnification: x200)
Western blot analysis of Human fetal muscle and brain tissue using TA321260(CUL4A Antibody) at dilution 1/450
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA321260(CUL4A Antibody)(left image) at dilution 1/22, the image on the right is treated with the synthetic peptide.(Original magnification: x200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302188 | Cullin-4A (N-term) antibody |
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| Rabbit Polyclonal Antibody against CUL4A (N-term) | |||||||
 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA319142 | Cullin-4A (N-term) antibody |
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| Rabbit polyclonal anti-Cul4A antibody | |||||||
 |
Rabbit | Serum | Hu, Ms | E, IP, P, WB |
0.1 ml /
€515.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA321259 | Cullin-4A antibody |
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| Anti-CUL4A Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA321260 | Cullin-4A antibody |
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| Anti-CUL4A Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AP07575SU-N | Cullin-4A antibody |
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 |
Rabbit | Hu | E, IP, P, WB |
50 µl /
€425.00
|
|||
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN203463 | CUL4A - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203463BN | CUL4A - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203463LP | CUL4A - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203463RB | CUL4A - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303463 | Cullin-4A (transcript variant 2) |
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| Purified recombinant protein of Homo sapiens cullin 4A (CUL4A), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY400388 | CUL4A overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. | ||
| LY418559 | CUL4A overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY429154 | CUL4A overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR209429L2V | Lenti ORF particles, Cul4a (GFP-tagged) - Mouse cullin 4A (Cul4a), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,550.00
|
|
| OriGene Technologies, Inc. | ||
| MR209429L1V | Lenti ORF particles, Cul4a (Myc-DDK-tagged) - Mouse cullin 4A (Cul4a), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,390.00
|
|
| OriGene Technologies, Inc. | ||