CYB5R2 antibody
Principal name
CYB5R2 antibody
Alternative names for CYB5R2 antibody
NADH-cytochrome b5 reductase 2, b5R.2
Ncbi ID
9606, NM_001014244, NP_057313, NP_057313.2, NM_016229, NP_001192156.1, NM_177216
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F)
Background of CYB5R2 antibody
- Kit Component:
- - KN304070G1, Cyb5r2 gRNA vector 1 in pCas-Guide vector
- KN304070G2, Cyb5r2 gRNA vector 2 in pCas-Guide vector
- KN304070D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Baker MA, Krutskikh A, Curry BJ, et al. (2005). "Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa". Biol. Reprod. 73 (2): 334–42.
Western Blot analysis of CYB5R2 expression in transfected 293T cell line (H00051700-T02) by CYB5R2 MaxPab polyclonal antibody.Lane 1: CYB5R2 transfected lysate(26.07 KDa).Lane 2: Non-transfected lysate.
Rat Lung; WB Suggested Anti-Cyb5r2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Rat Lung; Cyb5r2 antibody - C-terminal region (ARP53714_P050) in Rat Lung cells using Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R2 (RC217261, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R2.
HEK293T cells transfected with either RC217261 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CYB5R2 antibody(TA503251), and then analyzed by flow cytometry.
WB Suggested Anti-Cyb5r2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Rat Lung
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503251 | CYB5R2 antibody |
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| CYB5R2 mouse monoclonal antibody, clone OTI4B9 (formerly 4B9) | |||||||
 |
Mouse | IgG2a | OTI4B9 | Purified | Hu | F, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP317261 | CYB5R2 |
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| Recombinant protein of human cytochrome b5 reductase 2 (CYB5R2) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
