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Cyclin A2 antibody

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Principal name

Cyclin A2 antibody

Alternative names for Cyclin A2 antibody

Cyclin-A2, CCNA2, CCN1, CCNA, Cyclin A

SwissProt ID

P20248 (Human), P30274 (Bovin), P43449 (Chick), P47827 (Xenla), P51943 (Mouse)

Gene ID

890 (CCNA2), 12428 (Ccna2), 281667

Ncbi ID

NP_001228, NP_033958, 9606, NP_001228.1, 9913, NP_033958.2, NM_001237, NM_009828

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Hst (Hamster), Mink, Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), Mky (Monkey), Mam (Mammalia)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F)

Background of Cyclin A2 antibody

Kit Component:
- KN302803G1, Ccna2 gRNA vector 1 in pCas-Guide vector
- KN302803G2, Ccna2 gRNA vector 2 in pCas-Guide vector
- KN302803D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Bartolo, D. L. D., et al., Blood 111, 4731-4740 (2008).
2. Ciarallo, S., et al., Mol. Cell. Biol. 22, 2993-3002 (2002).
3. Sandhu, C., et al., Oncogene 19, 5314-5323 (2000).
4. Sandhu, C., et al., Cancer Res. 60, 2616-2622 (2000).
5. Slingerland, J. M., et al., Mol. Cell. Biol. 14, 3683-3694 (1994).
6. Adamczewski, J. P., et al., J. Virol. 67, 6551-6557 (1993)
Clone E67.1 is used in these references.

AM20281AF-N - Cyclin A2
Western blot analysis of Cyclin A expression in Jurkat cells (Lane 1), Raji cells (Lane 2), HeLa cells (Lane 3), WR19L cells (Lane 4) and PC12 cells (Lane 5) using AM20281AF-N Cyclin A antibody.
AM20282AF-N - Cyclin A2
Western blot analysis of Cyclin A expression in Jurkat (Lane 1), Raji (Lane 2), HeLa (Lane 3), WR19L (Lane 4) and PC12 (Lane 5) using AM20282AF-N Cyclin A antibody.
AM20282AF-N - Cyclin A2
Immunoprecipitation of Cyclin A from Jurkat cells with AM20282AF-N (Lane 1) or Mouse IgG (Lane 2). After immunoprecipitation with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with biotinylated anti-Cyclin A monoclonal antibody (AM20282BT-N). Jurkat crude lysate was resolved in Lane 3.
AM20282BT-N - Cyclin A2
Immunoprecipitation of Cyclin A from Jurkat cells with AM20282AF-N (Lane 1) or Mouse IgG2a Isotype Control (Lane 2). After immunoprecipitation with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with biotinylated anti-Cyclin A monoclonal antibody (AM20282BT-N).
ARP30159_P050 - Cyclin A2
Human Testis; Ccna2 antibody - C-terminal region (ARP30159_P050) validated by WB using testis; Ccna2 antibody - C-terminal region (ARP30159_P050) in Human Testis cells using Western Blot
ARP30159_P050 - Cyclin A2
Rat Muscle; WB Suggested Anti-Ccna2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Rat Muscle; Ccna2 antibody - C-terminal region (ARP30159_P050) in Rat Muscle cells using Western Blot
H00000890-B01 - Cyclin A2
Immunofluorescence of purified MaxPab antibody to CCNA2 on HeLa cell. [antibody concentration 10 ug/ml]
H00000890-B01 - Cyclin A2
Western Blot analysis of CCNA2 expression in transfected 293T cell line (H00000890-T01) by CCNA2 MaxPab polyclonal antibody.Lane 1: CCNA2 transfected lysate(47.52 KDa).Lane 2: Non-transfected lysate.
PAB0817 - Cyclin A2
Western blot expression of Ccna2 polyclonal antibody ( Cat # PAB0817 ) in Hela whole cell lysate.
PAB2867 - Cyclin A2
Western blot analysis of CCNA2 polyclonal antibody ( Cat # PAB2867 ) in HL-60 cell line lysates ( 35µg/lane ) . CCNA2 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
PAB9996 - Cyclin A2
Immunohistochemical staining with CCNA2 polyclonal antibody ( Cat # PAB9996 ) was diluted 1 : 500 to detect CCNA2 in human testis tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
AP06079PU-N - Cyclin A2
Immunohistochemical analysis in paraffin-embedded human lung carcinoma tissue using Cyclin A2 antibody (Cat.-No. AP06079PU-N).
AP06079PU-N - Cyclin A2
Western blot (WB) analysis of Cyclin A antibody (Cat.-No.: AP06079PU-N) at 1/500 dilution
Lane1:HEK293T cell lysate
Lane2:NIH-3T3 cell lysate
Lane3:Rat brain tissue lysate
AP14028PU-N - Cyclin A2
Western blot analysis of anti-CCNA2(N-term) Pab in HL60 cell line lysates (35ug/lane). CCNA2(arrow) was detected using the purified Pab (1:60 dilution).
AP14029PU-N - Cyclin A2
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CCNA2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP14029PU-N - Cyclin A2
Western blot analysis of CCNA2 (arrow) using rabbit polyclonal CCNA2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCNA2 gene (Lane 2)
AVARP03045_T100 - Cyclin A2
Human HepG2; WB Suggested Anti-CCNA2 Antibody Titration: 1ug/ml. Positive Control: HepG2 cell lysate. CCNA2 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; CCNA2 antibody - C-terminal region (AVARP03045_T100) in Human HepG2 cells using Western Blot
H00000890-D01 - Cyclin A2
Immunoprecipitation of CCNA2 transfected lysate using anti-CCNA2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CCNA2 MaxPab mouse polyclonal antibody (B01) (H00000890-B01).
AR50399PU-N - Cyclin A2
AM20281PU-S - Cyclin A2
HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with polyclonal anti-cyclin A antibody (clone E23.1). Proteins were visualized using a goat anti-mouse IgG conjugated to HRP and chemiluminescence detection.
TA590075 - Cyclin A2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCNA2 (RC211148, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCNA2.
TA590075 - Cyclin A2
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CCNA2 Polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
TA590075 - Cyclin A2
ELISA: Cyclin A2 Antibody
TP311148 - Cyclin A2
TA319146 - Cyclin A2
Western Blot of Rabbit anti- Cyclin A antibody. Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr). Lane 3: T98G cells treated with PIN1 knockdown (kd1). Load: 25 µg per lane. Primary antibody: Cyclin A antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 60kDa for Cyclin A.
TA319146 - Cyclin A2
Immunohistochemistry of Rabbit Anti-Cyclin A antibody. Tissue: human testes tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Cyclin A antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Cyclin A is nuclear and cytoplasmic. Staining: Cyclin A as precipitated red signal with hematoxylin purple nuclear counterstain.
TA330368 - Cyclin A2
WB Suggested Anti-CCNA2 Antibody Titration: 1 ug/ml; Positive Control: HepG2 cell lysateCCNA2 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells

14 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cyclin A2 antibody
Cyclin A2 Mouse IgG2a E67 Purified Hu, Mink, Mky, Ms C, F, ICC/IF, IP, P, WB
0.1 mg / €430.00
  OriGene Technologies GmbH
 

OriGene recommends this product for Immunohistochemistry.

Cyclin A2 antibody
Cyclin A2 Mouse IgG2a E67 Purified Hu, Mink, Mky, Ms C, F, ICC/IF, IP, P, WB
20 µg / €240.00
  OriGene Technologies GmbH
 

OriGene recommends this product for Immunohistochemistry.

Cyclin A2 antibody
  Rabbit polyclonal anti-Cyclin A antibody  
Western Blot of Rabbit anti- Cyclin A antibody. Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr). Lane 3: T98G cells treated with PIN1 knockdown (kd1). Load: 25 µg per lane. Primary antibody: Cyclin A antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 60kDa for Cyclin A. Rabbit Serum Hu, Ms, Rt E, P, WB
0.1 ml / €515.00
  OriGene Technologies, Inc.
+1 additional image

Cyclin A2 antibody
  Rabbit Polyclonal Cyclin A2 Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCNA2 (RC211148, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCNA2. Rabbit IgG Purified Hu, Mky E, WB
0.1 mg / €409.00
  OriGene Technologies, Inc.
+2 additional images

Cyclin A2 antibody
Western blot analysis of Cyclin A expression in Jurkat (Lane 1), Raji (Lane 2), HeLa (Lane 3), WR19L (Lane 4) and PC12 (Lane 5) using AM20282AF-N Cyclin A antibody. Mouse IgG2a E67.1 Azide Free Hu, Ms, Rt IP, WB
0.1 mg / €340.00
  OriGene Technologies GmbH
+1 additional image

Cyclin A2 antibody
Immunoprecipitation of Cyclin A from Jurkat cells with AM20282AF-N (Lane 1) or Mouse IgG2a Isotype Control (Lane 2). After immunoprecipitation with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with biotinylated anti-Cyclin A monoclonal antibody (AM20282BT-N). Mouse IgG2a E67.1 Biotin Hu, Ms, Rt IP, WB
1 ml / €375.00
  OriGene Technologies GmbH

Cyclin A2 antibody
Cyclin A2 Mouse IgG2a CY-28 Purified Hu, Ms ICC/IF, WB
0.1 mg / €390.00
  OriGene Technologies GmbH

Cyclin A2 antibody
Immunohistochemical analysis in paraffin-embedded human lung carcinoma tissue using Cyclin A2 antibody (Cat.-No. AP06079PU-N). Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

Cyclin A2 (N-term) antibody
Western blot analysis of anti-CCNA2(N-term) Pab in HL60 cell line lysates (35ug/lane). CCNA2(arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Cyclin A2 (C-term) antibody
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CCNA2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Cyclin A2
  Recombinant protein of human cyclin A2 (CCNA2)  
Cyclin A2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

CCNA2 overexpression lysate
CCNA2 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Ccna2 (GFP-tagged) - Mouse cyclin A2 (Ccna2), 200 uL, >10^7 TU/mL
Lenti ORF particles, Ccna2 (GFP-tagged) - Mouse cyclin A2 (Ccna2), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Ccna2 (Myc-DDK-tagged) - Mouse cyclin A2 (Ccna2), 200 uL, >10^7 TU/mL
Lenti ORF particles, Ccna2 (Myc-DDK-tagged) - Mouse cyclin A2 (Ccna2), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

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  2. Polyclonal (5)
Target
  1. Cyclin A2 (11)
Property
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Reactivity
  1. Human (Hu) (10)
  2. Mouse (Ms) (7)
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Applications
  1. Western blot / Immunoblot (WB) (10)
  2. Paraffin Sections (P) (5)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (4)
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  5. Enzyme Immunoassay (E) (3)
  6. Frozen sections (C) (2)
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Manufacturer
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