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Cyclin L1 antibody

Principal name

Cyclin L1 antibody

Alternative names for Cyclin L1 antibody

Cyclin-L1, Cyclin-L, CCNL1, BM-001

SwissProt ID

Q52KE7 (Mouse), Q5ZJP9 (Chick), Q6GN15 (Xenla), Q9R1Q2 (Rat), Q9UK58 (Human)

Gene ID

57018 (CCNL1), 56706 (Ccnl1), 114121

Ncbi ID

9606, NP_064703, NP_064703.1, NP_064321.2, NM_019937, NM_053662, NM_020307

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Chimp (Chimpanzee), Por (Porcine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)

Background of Cyclin L1 antibody

Cell proliferation is controlled at specific stages of the cell cycle by distinct protein kinase complexes. These complexes consist of a catalytic subunit associating with a specific regulatory subunit to form the active kinase. The cyclins, which include cyclin A, B, C, D, E, F, G, H, I, K, L, T and their related proteins, including Dbf4, comprise the regulatory subunits of these kinase complexes. The controlled activation of the kinase complexes at various intervals of the cell cycle is regulated by the availability of the cyclins to the catalytic subunit. Unlike the catalytic subunit, which is expressed continually, the expression and stability of the regulatory subunit fluctuates depending on the stage of the cell cycle and, thereby, regulates the kinase activity. Cyclin L1 is a ubiquitously expressed nuclear protein that can be detected in higher levels in thymus. In neck and head squamous cell carcinomas, cyclin L1 can be overexpressed and is therefore often considered a proto-oncogene. It interacts with POLR2A, CDC2L and SFRS2. Cyclin L1 plays a role in the mRNA splicing process regulation.

General readings

Clark,H.F., et al., (2003) Genome Res. 13 (10), 2265-2270

Immunofluorescence of monoclonal antibody to CCNL1 on HeLa cell. [antibody concentration 10 ug/ml]

H00057018-M01

Western Blot analysis of CCNL1 expression in transfected 293T cell line by CCNL1 monoclonal antibody (M01), clone 1F7-1C5.Lane 1: CCNL1 transfected lysate(18 KDa).Lane 2: Non-transfected lysate.

H00057018-M01

Detection limit for recombinant GST tagged CCNL1 is approximately 3ng/ml as a capture antibody.

H00057018-M01

Human HepG2; WB Suggested Anti-CCNL1 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate. CCNL1 is supported by BioGPS gene expression data to be expressed in HepG2.; CCNL1 antibody - N-terminal region (ARP33174_T100) in Human HepG2 cells using Western Blot

ARP33174_T100

Immunofluorescence of purified MaxPab antibody to CCNL1 on HeLa cell. [antibody concentration 10 ug/ml]

H00057018-B01

Western Blot analysis of CCNL1 expression in transfected 293T cell line (H00057018-T01) by CCNL1 MaxPab polyclonal antibody.Lane 1: CCNL1 transfected lysate(57.86 KDa).Lane 2: Non-transfected lysate.

H00057018-B01

Immunohistochemistry (IHC) analyzes of Cyclin-L1 antibody (Cat.-No.: AP06277PU-N) in paraffin-embedded human breast carcinoma lung carcinoma tissue.

AP06277PU-N

Western blot (WB) analysis of Cyclin-L1 antibody (Cat.-No.: AP06277PU-N) in extracts from HepG2 cells.

AP06277PU-N

Figure 1. Staining CCNL1 in Thymus by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.

AP07894PU-N

Figure 1. Staining CCNL1 in Skeletal Muscle by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.

AP07906PU-N

Western blot using CCNL1 polyclonal antibody ( Cat # PAB9945 ) shows detectionof a band ~59 kDa corresponding to a CCNL1 ( arrowhead ) present in mouse brain whole cell lysate.Approximately 35 µg of lysate was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1 : 3,500 for 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye^TM800 conjugated Gt-a-RabbitIgG [H&L] MX for 45 min at room temperature.IRDye^TM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.

PAB9945

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Cyclin L1 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HepG2 cells, using Cyclin L1 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemical analysis of Cyclin L1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of Cyclin L1 expression in HL60 (A); NIH3T3 (B); Jurkat (C); THP1 (D); K562 (E) whole cell lysates.

Western blot analysis of extracts from HepG2 cells, using Cyclin-L1 antibody (#TA315638).The lane on the right is treated with the synthesized peptide.

TA315638

Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using Cyclin-L1 Antibody. The lane on the left is treated with systhesized peptide.

TA315638

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin-L1 antibody (#TA315638).The picture on the right is treated with the synthesized peptide.

TA315638

WB Suggested Anti-CCNL1 Antibody Titration: 2.5ug/ml; ELISA Titer: 1: 62500; Positive Control: HepG2 cell lysateCCNL1 is supported by BioGPS gene expression data to be expressed in HepG2

TA331832

Western blot analysis on HepG2 cell lysate using Cyclin L1 Antibody

TA347454

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA315638	Cyclin L1 antibody
	Rabbit polyclonal anti-Cyclin-L1 antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
+2 additional images
TA347454	Cyclin L1 antibody
	Rabbit Polyclonal Anti-Cyclin L1 Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	WB	0.1 mg / €335.00
						OriGene Technologies, Inc.
AP06277PU-N	Cyclin L1 antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
						OriGene Technologies GmbH
+1 additional image
AP07894PU-N	Cyclin L1 antibody
	Rabbit		Purified	Hu	E, IP, P, WB	50 µg / €425.00
						OriGene Technologies GmbH
AP07906PU-N	Cyclin L1 antibody
	Rabbit		Purified	Hu, Ms, Rt	E, IP, P, WB	50 µg / €425.00
						OriGene Technologies GmbH

Mouse Lenti ORF Particles

Catalog No.
MR208526L2V	Lenti ORF particles, Ccnl1 (GFP-tagged) - Mouse cyclin L1 (Ccnl1), 200 uL, >10^7 TU/mL
		200 µl / €1,230.00
		OriGene Technologies, Inc.

MR208526L1V	Lenti ORF particles, Ccnl1 (Myc-DDK-tagged) - Mouse cyclin L1 (Ccnl1), 200 uL, >10^7 TU/mL
		200 µl / €1,100.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR214465L2V	Lenti ORF particles, Ccnl1 (GFP-tagged ORF) - Rat cyclin L1 (Ccnl1), 200 uL, >10^7 TU/mL
		200 µl / €1,220.00
		OriGene Technologies, Inc.

RR214465L1V	Lenti ORF particles, Ccnl1 (Myc-DDK-tagged ORF) - Rat cyclin L1 (Ccnl1), 200 uL, >10^7 TU/mL
		200 µl / €1,100.00
		OriGene Technologies, Inc.

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