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Cyclin T2 antibody

Principal name

Cyclin T2 antibody

Alternative names for Cyclin T2 antibody

Cyclin-T2, CycT2, CCNT2

SwissProt ID

O60583 (Human)

Gene ID

905 (CCNT2)

Ncbi ID

9606, NP_001232.1, NM_001241

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of Cyclin T2 antibody

Cyclin T2 is a ubiquitously expressed nuclear protein that is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns that contribute to the temporal coordination of each mitotic event. This cyclin and its kinase partner CDK9 were found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin was reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein. Two alternatively spliced transcript variants, which encode distinct isoforms, have been described. Transcript variant a (isoform a) lacks an internal fragment, which leads to a translation frame change, when compared to variant b (isoform b). The resulting isoform a protein lacks a C-terminal 88 aa, and has an unique 21 aa C-terminus, as compared to isoform b.

CCNT2 monoclonal antibody (M03), clone 1H3. Western Blot analysis of CCNT2 expression in Jurkat(Cat # L017V1 ).

H00000905-M03

Western blot using CCNT2 polyclonal antibody ( Cat # PAB11292 ) is shown to detect two major bands ( arrowheads ) corresponding to human CCN T2a and T2b as indicated.Approximately 33 µg of a HeLa whole cell lysate was separated by 4-20% Tris Glycine SDS-PAGE.After blocking the membrane with 5% BLOTTO in PBS, the membrane was probed forovernight at 4°C with the primary antibody diluted to 1 : 500 in 5% BLOTTO in PBS.The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye^TM800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature ( 800 nm channel, green ).Molecular weight estimation was made by comparison to prestained MW markers indicated at the right ( 700 nm channel, red ).IRDye^TM 800 fluorescenceimage was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is atrademark of LI-COR, Inc.

PAB11292

Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. TA307981 diluted at 1:1000.

TA307981

Immunohistochemical analysis of paraffin-embedded H1299 Xenograft, using cyclin T2(TA307981) antibody at 1:100 dilution.

TA307981

Immunofluorescence analysis of paraformaldehyde-fixed A431, using cyclin T2(TA307981) antibody at 1:200 dilution.

TA307981

WB using Anti-Cyclin T2 (Rabbit) to detect two major bands (arrowheads) corresponding to human Cyclin T2a and T2b as indicated. The primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (700 nm channel, red).

TA319406

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA307981	Cyclin T2 antibody
	Rabbit polyclonal antibody to cyclin T2 (cyclin T2)
	Rabbit	IgG	Aff - Purified	Hu	ICC/IF, P, WB	0.1 ml / €415.00
						OriGene Technologies, Inc.
+2 additional images
TA319406	Cyclin T2 (Isoform A+B) antibody
	Rabbit polyclonal Cyclin T2 antibody
	Rabbit	IgG	Serum	Hu	E, WB	0.1 ml / €515.00
						OriGene Technologies, Inc.

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