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CYP1A1 antibody

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Principal name

CYP1A1 antibody

Alternative names for CYP1A1 antibody

Cytochrome P450 1A1, Cytochrome P450-P1, Cytochrome P450 form 6, Cytochrome P450-C

SwissProt ID

P00184 (Mouse), P00185 (Rat), P04798 (Human), P05176 (Rabit), P56590 (Canfa), P56591 (Sheep), Q06367 (Cavpo), Q5KQT7 (Felca)

Gene ID

1543 (CYP1A1), 24296 (Cyp1a1), 13076

Ncbi ID

NP_000490, 9606, 10116, NP_000490.1, NP_001129531.1, NM_009992, NM_012540, NM_000499

Available reactivities

Hu (Human), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Can (Canine), Mky (Monkey), Sh (Sheep), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Ze (Zebrafish), Gt (Goat)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Frozen Sections (C)

Background of CYP1A1 antibody

CYP1A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme\'s endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. CYP1A1 has been associated with lung cancer risk. This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme\'s endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Ikeda,S., (2008) Am. J. Gastroenterol. 103 (6), 1476-1487

ARP41404_P050 - CYP1A1
Human kidney; . Sample Type: Human Kidney. Dilution:. 1:100; CYP1A1 antibody - middle region (ARP41404_P050) in Human kidney cells using Immunohistochemistry
ARP41404_P050 - CYP1A1
Human HeLa; WB Suggested Anti-CYP1A1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Hela cell lysate; CYP1A1 antibody - middle region (ARP41404_P050) in Human HeLa cells using Western Blot
ARP41405_P050 - CYP1A1
Human lung microsome; . Lanes: Lane 1: Human lung microsome lysate. Lane 2-5: 150 ug mouse lung microsome lysate. Primary Antibody Dilution: 1: 1000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1: 10000. Gene Name: CYP1A1. Submitted by: Jing Peng, Fox Chase Cancer Center.; CYP1A1 antibody - middle region (ARP41405_P050) in Human lung microsome cells using Western Blot
ARP41405_P050 - CYP1A1
Human Heart; WB Suggested Anti-CYP1A1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human heart; CYP1A1 antibody - middle region (ARP41405_P050) in Human Heart cells using Western Blot
AP14897PU-N - CYP1A1
Figure 2. Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CYP1A1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP14897PU-N - CYP1A1
Figure 1. (LEFT) Western blot analysis of anti-CYP1A1 Antibody (C-term) (Cat#AP14897PU-N) in K562 cell line lysates (35µg/lane). CYP1A1(arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of anti-CYP1A1 Antibody (C-term) (cat#AP14897PU-N) in mouse lung tissue lysates (35µg/lane). CYP1A1(arrow) was detected using the purified Pab.
AP14898PU-N - CYP1A1
Western blot analysis of anti-CYP1A1 Antibody (Center) in mouse lung tissue lysates (35ug/lane). CYP1A1 (arrow) was detected using the purified Pab.
H00001543-D01P - CYP1A1
Western Blot analysis of CYP1A1 expression in transfected 293T cell line (H00001543-T02) by CYP1A1 MaxPab polyclonal antibody.Lane 1: CYP1A1 transfected lysate(58.20 KDa).Lane 2: Non-transfected lysate.
AP20396PU-N - CYP1A1
Western blot (WB) analysis of CYP1A1/2 Antibody Cat.-No AP20396PU-N in extracts from RAW264.7 cells.
AP20396PU-N - CYP1A1
Immunohistochemistry analysis of CYP1A1/2 Antibody Cat.-No AP20396PU-N in paraffin-embedded human liver carcinoma tissue at 1/50 showing cytoplasmic staining. Negative control (the right) using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit Ig
C12249-1 - CYP1A1
Western blot analysis of extracts from COS7/RAW264.7/HT-29 cells, using Cytochrome P450 1A1/2 Antibody. The lane on the right is treated with the synthesized peptide.
AM06716PU-N - CYP1A1
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CYP1A1 mouse mAb with DAB staining.
AM06716PU-N - CYP1A1
Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CYP1A1 mouse mAb with DAB staining.
AM06716PU-N - CYP1A1
Western blot analysis using CYP1A1 mAb against human CYP1A1 (AA: 203-461) recombinant protein. (Expected MW is 60 kDa)
AM06716PU-N - CYP1A1
Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)
CPA1307-100ul - Cytochrome P450 1A1/2
Immunohistochemical analysis of Cytochrome P450 1A1/2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA1307-100ul - Cytochrome P450 1A1/2
Immunofluorescent analysis of Cytochrome P450 1A1/2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CPA1307-100ul - Cytochrome P450 1A1/2
Western blot analysis of Cytochrome P450 1A1/2 expression in MCF7 (A); HeLa (B) whole cell lysates.
TP305760 - CYP1A1
TA309533 - CYP1A1
TA309533 (0.1µg/ml) staining of Rat Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA311781 - CYP1A1
Western blot analysis of extracts from COS-7 cells, RAW264.7 cells and HT-29 cells, using Cytochrome P450 1A1/2 antibody.The lane on the right is treated with the synthesized peptide.
TA321142 - CYP1A1
Gel: 10%SDS-PAGE, Lysate: 30 µg, Lane: Mouse liver tissue, Primary antibody:CYP1A1 Antibody at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
TA321142 - CYP1A1
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CYP1A1 Antibody at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
TA327303 - CYP1A1
Western blot analysis of extracts of various cell lines, using CYP1A1 antibody.
TA329676 - CYP1A1
TA329676 (0.3ug/ml) staining of A549 (A) and MCF7 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA346040 - CYP1A1
WB Suggested Anti-CYP1A1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Hela cell lysate
TA346040 - CYP1A1
Sample Type: Human Kidney; Dilution: 1: 100
TA346041 - CYP1A1
WB Suggested Anti-CYP1A1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human heart
TA346041 - CYP1A1
Lanes: Lane 1: Human lung microsome lysate; Lane 2-5: 150 ug mouse lung microsome lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-rabbit HRP; Secondary Antibody Dilution: 1: 10000; Gene Name: CYP1A1; Submitted by: Jing Peng, Fox Chase Cancer Center;
TA347969 - CYP1A1
Western blot analysis of Cytochrome P450 1A1/2 expression in RAW264.7 cells
TA352863 - CYP1A1
Western Blot of recombinant CYP450s
TA352863 - CYP1A1
Immunohistochemistry staining of human liver tissue with clone MC1.
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15 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CYP1A1 antibody
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CYP1A1 mouse mAb with DAB staining. Mouse IgG1 6G5 Purified Hu E, P, WB
0.1 mg / €410.00
  OriGene Technologies GmbH
+3 additional images

Highly validated antibody!

CYP1A1 antibody
  Goat Polyclonal Antibody against cytochrome P450 1A1 (mouse)  
TA309533 (0.1µg/ml) staining of Rat Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Ms, Rt E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

CYP1A1 antibody
  Rabbit polyclonal Cytochrome P450 1A1/2 antibody  
Western blot analysis of extracts from COS-7 cells, RAW264.7 cells and HT-29 cells, using Cytochrome P450 1A1/2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

CYP1A1 antibody
  Anti-CYP1A1 Rabbit Polyclonal Antibody  
Gel: 10%SDS-PAGE, Lysate: 30 µg, Lane: Mouse liver tissue, Primary antibody:CYP1A1 Antibody at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

CYP1A1 antibody
  Rabbit anti-CYP1A1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using CYP1A1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

CYP1A1 antibody
  Goat Anti-cytochrome P450 1A1 Polyclonal Antibody  
TA329676 (0.3ug/ml) staining of A549 (A) and MCF7 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

CYP1A1 antibody
  Rabbit Polyclonal Anti-Cytochrome P450 1A1/2 Antibody  
Western blot analysis of Cytochrome P450 1A1/2 expression in RAW264.7 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

CYP1A1 (C-term) antibody
Figure 2. Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CYP1A1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

CYP1A1 (C-term) antibody
Western blot analysis of anti-CYP1A1 Antibody (Center) in mouse lung tissue lysates (35ug/lane). CYP1A1 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

CYP1A1 (+CYP1A2) antibody
Western blot (WB) analysis of CYP1A1/2 Antibody Cat.-No AP20396PU-N in extracts from RAW264.7 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

CRISPR

Catalog No. Species Donor Vector  

CYP1A1 - human gene knockout kit via CRISPR
CYP1A1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CYP1A1 - human gene knockout kit via CRISPR
CYP1A1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CYP1A1
  Recombinant protein of human cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1)  
CYP1A1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Cyp1a1 (GFP-tagged ORF) - Rat cytochrome P450, family 1, subfamily a, polypeptide 1 (Cyp1a1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Cyp1a1 (GFP-tagged ORF) - Rat cytochrome P450, family 1, subfamily a, polypeptide 1 (Cyp1a1), 200 uL, >10^7 TU/mL  
200 µl / €1,220.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Cyp1a1 (Myc-DDK-tagged ORF) - Rat cytochrome P450, family 1, subfamily a, polypeptide 1 (Cyp1a1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Cyp1a1 (Myc-DDK-tagged ORF) - Rat cytochrome P450, family 1, subfamily a, polypeptide 1 (Cyp1a1), 200 uL, >10^7 TU/mL  
200 µl / €1,090.00
  OriGene Technologies, Inc.
 

15 Item(s)

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15 products - FILTER YOUR RESULTS!

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  2. Monoclonal (1)
Target
  1. CYP1A1 (11)
Property
  1. +CYP1A2 (1)
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Reactivity
  1. Human (Hu) (9)
  2. Mouse (Ms) (7)
  3. Rat (Rt) (6)
Applications
  1. Western blot / Immunoblot (WB) (10)
  2. Enzyme Immunoassay (E) (5)
  3. Paraffin Sections (P) (4)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
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  2. 3+ images (1)
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Service
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