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CYP26A1 antibody

Principal name

CYP26A1 antibody

Alternative names for CYP26A1 antibody

Cytochrome P450 26A1, CYP26, Cytochrome P450RAI1, Retinoic acid-metabolizing cytochrome, P450 retinoic acid-inactivating 1, Retinoic acid 4-hydroxylase

SwissProt ID

O43174 (Human), O55127 (Mouse), O93323 (Xenla), Q9PUB4 (Chick)

Gene ID

1592 (CYP26A1), 13082 (Cyp26a1)

Ncbi ID

NP_000774.2, 9606, NP_000774, NM_000783, NM_057157, NP_031837.2

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bt (Bat), Can (Canine), Hst (Hamster), Mink, Prim (Primate), Rb (Rabbit), Bear, Bov (Bovine), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of CYP26A1 antibody

The cytochrome P450 proteins (CYPs) are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. P450 enzymes are classified into subfamilies based on their sequence similarities. CYP26A1, also referred to as retinoic acid-4-hydroxylase, is a major retinoic acid catabolic enzyme. CYP26A1 plays an important role in protecting tailbud tissues from inappropriate exposure to retinoic acid. CYP26A1 transcription is epigenetically regulated by nuclear retinoic acid receptors 2. Mutations in the gene encoding for CYP26A1 are associated with caudal agenesis and spina bifida, imperforate anus, agenesis of the caudal portions of the digestive and urogenital tracts, and malformed lumbosacral skeletal elements. CYP26A1 is upregulated in adenomatous polyposis coli mouse adenomas, human FAP adenomas, human sporadic colon carcinomas, and in the intestine of adenomatous polyposis coli(mcr) mutant zebrafish embryos.

Figure 1. Immunohistochemical staining of liposarcoma cells using CYP26A1 antibody (cat. No. AM05374PU-N).

AM05374PU-N

Figure 2. Western blot on recombinant protein corresponding to C-terminal region of CYP26A1 protein using CYP26A1 antibody (AM05374PU-N).

AM05374PU-N

Western blot analysis of CYP26A1 polyclonal antibody ( Cat # PAB2601 ) in Jurkat cell line lysates ( 35 µg/lane ) . CYP26A1 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .

PAB2601

Western Blot analysis of CYP26A1 expression in transfected 293T cell line (H00001592-T01) by CYP26A1 MaxPab polyclonal antibody.Lane 1: CYP26A1 transfected lysate(47.08 KDa).Lane 2: Non-transfected lysate.

H00001592-B01P

Western blot analysis of anti-CYP26A1 Antibody (Center) (Cat.#AP12392PU-N) in Jurkat cell line lysates (35ug/lane). CYP26A1 (arrow) was detected using the purified Pab (1:60dilution).

AP12392PU-N

Western blot on recombinant protein corresponding to C-terminal region of CYP26A1 protein using CYP26A1 monoclonal antibody, clone F27 P6 A1 ( Cat # MAB1857 ).

MAB1857

Western blot analysis of extracts from HepG2/HeLa/293 cells, using Cytochrome P450 26A1 Antibody. The lane on the right is treated with the synthesized peptide.

Brain, cortex, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP23113PU-N

Brain, cerebellum, white matter, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP23113PU-N

Immunofluorescent analysis of Cytochrome P450 26A1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of Cytochrome P450 26A1 expression in HepG2 (A); HEK293T (B) whole cell lysates.

TP316278

Western blot analysis of extracts from HepG2 cells, HeLa cells and 293 cells, using Cytochrome P450 26A1 antibody.The lane on the right is treated with the synthesized peptide.

TA311699

Western blot analysis on HepG2 cell lysate using Cytochrome P450 26A1 Antibody

TA347614

TA348981 (2.5 ug/ml) staining of PFA-fixed and TX100-treated HeLa cells after primary labelling overnight at 4? with DAPI nuclear counterstaining in blue.

TA348981

Western Blot analysis of recombinant P450 26A1 C-terminus region. Use 1/5 supernatant dilution.

TA353120

Western blot analysis of cell lysate overexpressing CYP26A1.

TA353120

Immunohistochemistry staining of liposarcoma cells with clone F27P6A1. Anti-Cytochrome P450 26A1 can be used neat or 1/5 diluted for IHC; antigen retrieval is through microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0

TA353120

Immunohistochemistry staining of normal tissue, liposarcoma and secondary lesions. Clone F27P6A1 can be used neat or 1/5 diluted; antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0

TA353120

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA311699	CYP26A1 antibody
	Rabbit polyclonal Cytochrome P450 26A1 antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
TA347614	CYP26A1 antibody
	Rabbit Polyclonal Anti-Cytochrome P450 26A1 Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	WB	0.1 mg / €335.00
						OriGene Technologies, Inc.
TA348981	CYP26A1 antibody
	Goat Polyclonal Anti-CYP26A1 Antibody
	Goat		Aff - Purified	Hu	E, ICC/IF, P, WB	0.1 mg / €360.00
						OriGene Technologies, Inc.
AP12392PU-N	CYP26A1 antibody
	Rabbit	Ig	Purified	Hu	WB	0.4 ml / €415.00
						OriGene Technologies GmbH
AP23113PU-N	CYP26A1 (485-496) antibody
	Goat		Aff - Purified	Elephant, Hu	P	50 µg / €425.00
						OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP316278	CYP26A1 (transcript variant 1)
	Recombinant protein of human cytochrome P450, family 26, subfamily A, polypeptide 1 (CYP26A1), transcript variant 1
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY400267	CYP26A1 overexpression lysate
		0.1 mg / €495.00
		OriGene Technologies, Inc.
LY409260	CYP26A1 overexpression lysate
		0.1 mg / €495.00
		OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.
RC216278L1V	Lenti ORF particles, CYP26A1 (Myc-DDK tagged) - Human cytochrome P450, family 26, subfamily A, polypeptide 1 (CYP26A1), transcript variant 1 , 200 uL, >10^7 TU/mL
		200 µl / €1,060.00
		OriGene Technologies, Inc.

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