CYP2A6 antibody
Principal name
CYP2A6 antibody
Alternative names for CYP2A6 antibody
CYP2A3,Cytochrome P450 2A6, CYPIIA6, CYPIIA3, Coumarin 7-hydroxylase
SwissProt ID
P11509 (Human)
Gene ID
1548 (CYP2A6)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F)
Background of CYP2A6 antibody
This gene, CYP2A6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to hydroxylate coumarin, and also metabolizes nicotine, aflatoxin B1, nitrosamines, and some pharmaceuticals. Individuals with certain allelic variants are said to have a poor metabolizer phenotype, meaning they do not efficiently metabolize coumarin or nicotine. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. The gene was formerly referred to as CYP2A3; however, it has been renamed CYP2A6. [provided by RefSeq, Jul 2008].
Western blot (WB) analyzes of CYP2A6 antibody (Cat.-No.: AP06774PU-N) in extracts from Jurkat cells.
Left: Western blot using CYP2A6 antibody on recombinant cytochrome p450 at 1 µg/ml. Right: Staining using CYP2A6 antibody on normal human liver tissue at 1 µg/ml.
Western blot analysis using CYP2A6 monoclonal antibody, clone F16 P2 D8 ( Cat # MAB1858 ) on recombinant CYP2A6 proteins at 1 µg/ml.
Immunohistochemistry staining using CYP2A6 monoclonal antibody, clone F16 P2 D8 ( Cat # MAB1858 ) on normal human liver tissue at 1 µg/ml.
CYP2A6 MaxPab polyclonal antibody. Western Blot analysis of CYP2A6 expression in human liver.
Western Blot analysis of CYP2A6 expression in transfected 293T cell line (H00001548-T01) by CYP2A6 MaxPab polyclonal antibody.Lane 1: CYP2A6 transfected lysate(54.34 KDa).Lane 2: Non-transfected lysate.
CYP2A6 MaxPab rabbit polyclonal antibody. Western Blot analysis of CYP2A6 expression in human liver.
Western Blot analysis of CYP2A6 expression in transfected 293T cell line (H00001548-T02) by CYP2A6 MaxPab polyclonal antibody.Lane 1: CYP2A6 transfected lysate(56.50 KDa).Lane 2: Non-transfected lysate.
Human COLO205; WB Suggested Anti-CYP2A6 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: COLO205 cell lysate; CYP2A6 antibody - C-terminal region (ARP60126_P050) in Human COLO205 cells using Western Blot
Western blot analysis of Cytochrome P450 2A6 expression in HepG2 (A) whole cell lysates.
Immunofluorescent analysis of Cytochrome P450 2A6 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Cytochrome P450 2A6 expression in HeLa (A); Jurkat (B); MCF7 (C) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CYP2A6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503805)
Anti-CYP2A6 mouse monoclonal antibody (TA503805) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP2A6(RC222995).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2A6 (RC222995, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2A6.
HEK293T cells transfected with either RC222995 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CYP2A6 antibody(TA503805), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CYP2A6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503827)
Anti-CYP2A6 mouse monoclonal antibody (TA503827) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP2A6(RC222995).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2A6 (RC222995, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2A6.
HEK293T cells transfected with either RC222995 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CYP2A6 antibody(TA503827), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CYP2A6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503832)
Anti-CYP2A6 mouse monoclonal antibody (TA503832) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP2A6(RC222995).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2A6 (RC222995, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2A6.
HEK293T cells transfected with either RC222995 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CYP2A6 antibody(TA503832), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CYP2A6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503857)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CYP2A6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503857)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CYP2A6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503857)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CYP2A6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503857)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CYP2A6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503857)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-CYP2A6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503857)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CYP2A6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503857)
Western blot analysis of extracts (10ug) from 7 Human tissue by using anti-CYP2A6 monoclonal antibody ( 1: Uterus; 2: Breast; 3: Brain; 4: Liver; 5: Ovary; 6: Thyroid gland; 7: colon) at 1:200 dilution.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2A6 (RC222995, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2A6.
Flow cytometric Analysis of Jurkat cells, using anti-CYP2A6 antibody(TA503857),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CYP2A6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504099)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CYP2A6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504099)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CYP2A6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504099)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2A6 (RC222995, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2A6.
Western blot analysis of extracts from Jurkat cells, using Cytochrome P450 2A6 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts of various cell lines, using CYP2A6 antibody.
WB Suggested Anti-CYP2A6 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: COLO205 cell lysate
WB Suggested Anti-CYP2A6 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Small Intestine
Western Blot of recombinant CYP450.
Immunohistochemistry staining of human liver tissue with clone F16 P2 D8.
Western Blot of recombinant CYP450s
- Quick-Filters:
- Highly Validated (5)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503857 | CYP2A6 antibody |
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| CYP2A6 (Cytochrome P450 2A6) mouse monoclonal antibody, clone OTI1D2 (formerly 1D2) | |||||||
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Mouse | IgG2b | OTI1D2 | Purified | Hu, Mky, Ms, Rt | F, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +10 additional images |
Top Selling product! Highly validated antibody!
|
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| TA503805 | CYP2A6 antibody |
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| CYP2A6 (Cytochrome P450 2A6) mouse monoclonal antibody, clone OTI5F8 (formerly 5F8) | |||||||
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Mouse | IgG2b | OTI5F8 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA503827 | CYP2A6 antibody |
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| CYP2A6 (Cytochrome P450 2A6) mouse monoclonal antibody, clone OTI2A8 (formerly 2A8) | |||||||
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Mouse | IgG2b | OTI2A8 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA503832 | CYP2A6 antibody |
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| CYP2A6 (Cytochrome P450 2A6) mouse monoclonal antibody, clone OTI3C1 (formerly 3C1) | |||||||
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Mouse | IgG2a | OTI3C1 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA504099 | CYP2A6 antibody |
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| CYP2A6 (Cytochrome P450 2A6) mouse monoclonal antibody, clone OTI9G3 (formerly 9G3) | |||||||
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Mouse | IgG2b | OTI9G3 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA313312 | CYP2A6 antibody |
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| Rabbit polyclonal Cytochrome P450 2A6 antibody | |||||||
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Rabbit | IgG | Purified | Hu | WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA332933 | CYP2A6 antibody |
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| Rabbit anti-CYP2A6 Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| AM05370PU-N | CYP2A6 antibody |
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Mouse | IgG1 | F16 P2 D8 | Purified | Hu | C, E, WB |
0.2 mg /
€400.00
|
| OriGene Technologies GmbH | |||||||
| AP06774PU-N | CYP2A6 antibody |
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Rabbit | Aff - Purified | Hu | ICC/IF, WB |
0.1 mg /
€370.00
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| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP322995 | CYP2A6 |
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| Recombinant protein of human cytochrome P450, family 2, subfamily A, polypeptide 6 (CYP2A6) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY424530 | CYP2A6 overexpression lysate |
|
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0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
