CYP2B6 antibody
Principal name
CYP2B6 antibody
Alternative names for CYP2B6 antibody
Cytochrome P450 2B6, CYPIIB6, Cytochrome P450 IIB1
SwissProt ID
P20813 (Human)
Gene ID
1555 (CYP2B6)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)
Background of CYP2B6 antibody
This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008].
General readings
1. Shah A et al. HIV gp120- and methamphetamine-mediated oxidative stress induces astrocyte apoptosis via cytochrome P450 2E1. Cell Death Dis 4:e850 (2013). Human .
Human THP-1; WB Suggested Anti-CYP2B6 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: THP-1 cell lysate; CYP2B6 antibody - middle region (ARP41805_P050) in Human THP-1 cells using Western Blot
Immunohistochemical analysis of Cytochrome P450 2B6 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Cytochrome P450 2B6 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Cytochrome P450 2B6 expression in HepG2 (A) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504328)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504328)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504328)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504328)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504328)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504328)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504328)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504328)
Anti-CYP2B6 mouse monoclonal antibody (TA504328) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP2B6(RC217236).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CYP2B6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2B6 (RC217236, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2B6.
HEK293T cells transfected with either RC217236 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CYP2B6 antibody(TA504328), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CYP2B6 antibody(TA504328),(Red), compared to a nonspecific negative control antibody,(Blue).
CYP2B6 Antibody (Center) (Cat. #TA324379) western blot analysis in 293(lane 1) and K562(lane 2) cell line lysates (35ug/lane).This demonstrates the CYP2B6 antibody detected the CYP2B6 protein (arrow).
CYP2B6 Antibody (Center) (Cat. #TA324379)immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP2B6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of CYP2B6 Antibody (Center)(Cat#TA324379) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis on HT29 cell lysate using Cytochrome P450 2B6 Antibody
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human colon cancer tissue, Primary antibody: (CYP2B6 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 4 minutes
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using (CYP2B6 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
WB Suggested Anti-CYP2B6 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: THP-1 cell lysate
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP317236 | CYP2B6 |
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| Recombinant protein of human cytochrome P450, family 2, subfamily B, polypeptide 6 (CYP2B6) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||