CYP3A4 antibody
Principal name
CYP3A4 antibody
Alternative names for CYP3A4 antibody
CYP3A3, Cytochrome P450 3A4, CYPIIIA4, Nifedipine oxidase, Cytochrome P450 3A3, CYPIIIA3, Cytochrome P450 HLp, Cytochrome P450 NF-25, Cytochrome P450-PCN1, Albendazole monooxygenase, Albendazole sulfoxidase, Quinine 3-monooxygenase, Taurochenodeoxycholate 6-alpha-hydroxylase
SwissProt ID
P08684 (Human)
Gene ID
1576 (CYP3A4)
Ncbi ID
Available reactivities
Available hosts
Available applications
Functional assay (FN), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F), Frozen Sections (C)
Background of CYP3A4 antibody
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2011].
Western blot(WB) analyzes of CYP3A4/5 antibody (Cat.-No.: AP06777PU-N) in extracts from Jurkat cells
Immunoperoxidase of purified MaxPab antibody to CYP3A4 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
CYP3A4 MaxPab polyclonal antibody. Western Blot analysis of CYP3A4 expression in human liver.
Western Blot analysis of CYP3A4 expression in transfected 293T cell line (H00001576-T01) by CYP3A4 MaxPab polyclonal antibody.Lane 1: CYP3A4 transfected lysate(55.33 KDa).Lane 2: Non-transfected lysate.
CYP3A4 MaxPab polyclonal antibody. Western Blot analysis of CYP3A4 expression in HepG2.
Western Blot analysis of CYP3A4 expression in transfected 293T cell line (H00001576-T01) by CYP3A4 MaxPab polyclonal antibody.Lane 1: CYP3A4 transfected lysate(57.30 KDa).Lane 2: Non-transfected lysate.
CYP3A4 MaxPab rabbit polyclonal antibody. Western Blot analysis of CYP3A4 expression in HepG2.
Western blot analysis of anti-CYP3A4 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). CYP3A4 (arrow) was detected using the purified Pab.
Lane 1 : molecular weight markers.Lane 2 : CYP3A4 -100 ng.Lane 3 : CYP3A4-300 ng.
Human THP-1; WB Suggested Anti-CYP3A4 Antibody Titration: 0.2-1 ug/ml. Positive Control: THP-1 cell lysate; CYP3A4 antibody - middle region (ARP51349_P050) in Human THP-1 cells using Western Blot
Human kidney; WB Suggested Anti-CYP3A4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human kidney; CYP3A4 antibody - middle region (ARP51348_P050) in Human kidney cells using Western Blot
Immunohistochemical analysis of paraffin-embedded liver cancer tissues using CYP3A4 Mouse mAb Cat.-No AM06743PU-N with DAB staining.
Immunohistochemical analysis of paraffin-embedded human brain tissues using CYP3A4 Mouse mAb Cat.-No AM06743PU-N with DAB staining.
Immunofluorescence analysis of HepG2 cells using CYP3A4 Mouse mAb Cat.-No AM06743PU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot analysis using CYP3A4 Mouse mAb Cat.-No AM06743PU-N against human CYP3A4 (AA: 243-430) recombinant protein. (Expected MW is 47.5 kDa)
Western blot analysis using CYP3A4 Mouse mAb Cat.-No AM06743PU-N against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.
Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)
Flow cytometric analysis of HepG2 cells using CYP3A4 Mouse mAb Cat.-No AM06743PU-N (green) and negative control (red).
Western blot analysis of Cytochrome P450 3A4 expression in NCIH460 (A); human liver (B) whole cell lysates.
Immunohistochemical analysis of Cytochrome P450 3A4/5 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Cytochrome P450 3A4/5 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Cytochrome P450 3A4/5 expression in NCIH460 (A); HepG2 (B) whole cell lysates.
Western blot analysis of extracts from Jurkat cells, using Cytochrome P450 3A4/5 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from K562 cells, using P450 3A4 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Cytochrome P450 3A4 antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of Human fetal liver tissue using TA324141(CYP3A4 Antibody) at dilution 1/250
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA324141(CYP3A4 Antibody)(left image) at dilution 1/40, the image on the right is treated with the fusion protein.(Original magnification: x200)
Immunohistochemistry of paraffin-embedded Human brain tissue using TA324141(CYP3A4 Antibody)(left image) at dilution 1/40, the image on the right is treated with the fusion protein.(Original magnification: x200)
Predicted band size: 57 kDa . Positive control: Mouse liver tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds)
Predicted cell location: Cytoplasm. Positive control: Human liver cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CYP3A4 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification: x200)
WB Suggested Anti-CYP3A4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human kidney
WB Suggested Anti-CYP3A4 Antibody Titration: 0.2-1 ug/ml; Positive Control: THP-1 cell lysate
Western Blot of recombinant CYP450s
Immunohistochemistry: antibody staining of prostate cancer tissue
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- Highly Validated (1)
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM06743PU-N | CYP3A4 antibody |
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Mouse | IgG1 | 3H8 | Purified | Hu | E, F, ICC/IF, P, WB |
0.1 mg /
€410.00
|
| OriGene Technologies GmbH | |||||||
 +6 additional images |
Highly validated antibody! |
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| TA313729 | CYP3A4 antibody |
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| Rabbit polyclonal Cytochrome P450 3A4/5 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA313730 | CYP3A4 antibody |
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| Rabbit polyclonal Cytochrome P450 3A4 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA324141 | CYP3A4 antibody |
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| Anti-CYP3A4 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA324142 | CYP3A4 antibody |
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| Anti-CYP3A4 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AP06777PU-N | CYP3A4 (+ CYP3A5) antibody |
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 |
Rabbit | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| AP14581PU-N | CYP3A4 antibody |
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 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| SP1332A | CYP3A4 antibody |
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 |
Rabbit | Azide Free | Hu | FN, WB |
50 µl /
€740.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP310170 | CYP3A4 |
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| Recombinant protein of human cytochrome P450, family 3, subfamily A, polypeptide 4 (CYP3A4) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||