DAB1 antibody
Principal name
DAB1 antibody
Alternative names for DAB1 antibody
Disabled homolog 1
Ncbi ID
NP_066566.3, NP_034144, 10090, NP_034144.1, NM_021080, NP_066566, NM_010014, NM_177259, NM_153621
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)
Background of DAB1 antibody
Dab1, a homolog of the Drosophila Disabled protein, is an adaptor protein involved in neural development. This cytoplasmic protein is tyrosine-phosphorylated during rapid expansion of the developing nervous system, and it is thought to interact with other proteins via a domain similar to the PTB domains of the Shc family. Dab1 has been shown to interact with the SH2 domains of Src, Fyn and Abl. Mutations in Dab1 result in widespread abnormalities in the brain, similar to those seen in Reelin mutants. Reelin is a secreted protein thought to play a role in directing migrating neurons. Evidence suggests that Dab1 functions downstream of Reelin in a signaling pathway involved in positioning cells in the developing brain. Dab2 (also designated DOC-2) is a mitogen-responsive phosphoprotein that binds the SH3 domain of Grb2, and it is thought to be a negative regulator of growth.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Dab1 antibody.
Western Blot analysis of DAB1 expression in transfected 293T cell line (H00001600-T01) by DAB1 MaxPab polyclonal antibody.Lane 1: DAB1 transfected lysate(60.83 KDa).Lane 2: Non-transfected lysate.
Western blot analysis usingDisabled-1 (NT) (1) and (CT) (2) on mouse brain extracts.
Western blot analysis using Disabled-1 (NT) (1) and (CT) (2) on mouse brain extracts.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using DAB1 polyclonal antibody ( Cat # PAB5309 ) .
Western Blot analysis of DAB1 expression in transfected 293T cell line (H00001600-T01) by DAB1 MaxPab polyclonal antibody.Lane 1: DAB1 transfected lysate(60.83 KDa).Lane 2: Non-transfected lysate.
Western blot analysis using Dab1 polyclonal antibody mouse brain extracts.
Western blot analysis using Dab1 polyclonal antibody mouse brain extracts.
Human THP-1; WB Suggested Anti-DAB1 Antibody Titration: 0.2-1 ug/ml. Positive Control: THP-1 cell lysate; DAB1 antibody - middle region (ARP52396_P050) in Human THP-1 cells using Western Blot
Western blot (WB) analyzes of Dab1 antibody (Cat.-No.: AP20741PU-N) in extracts from HeLa cells.
Western blot (WB) analyzes of Dab1 antibody (Cat.-No.: AP20742PU-N) in extracts from HepG2 cells.
Immunohistochemical staining of Formalin-fixed, Paraffin-embedded human intestine using anti-Dab1 antibody cat. no. AP39027PU-N
Immunofluorescence of MCF7 cells, using anti-Dab1 antibody cat. no. AP39027PU-N. Cells on the right are treated with the synthesized peptide.
Anti-Dab1 antibody cat. no. AP39027PU-N used in Western blot of extracts from HepG2 (left lane) and 293 cells (middle lane). Right lane: HepG2 cells treated with the sythesized peptide.
Immunofluorescent analysis of DAB1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of DAB1 expression in MCF7 (A); HEK293T (B) whole cell lysates.
Western blot analysis of Dab1 expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
WB Suggested Anti-DAB1 Antibody Titration: 0.2-1 ug/ml; Positive Control: THP-1 cell lysate
Western blot analysis of Dab1 phosphorylation expression in LOVO whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP05070PU-N | DAB1 (N-term) antibody |
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Sheep | IgG | Purified | Ms | WB |
0.25 mg /
€400.00
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| OriGene Technologies GmbH | |||||||
Published recently! |
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| TA325406 | DAB1 antibody |
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| Rabbit Polyclonal Dab1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA325407 | DAB1 antibody |
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| Rabbit Polyclonal Dab1 (Tyr232) Antibody (Phospho-specific) | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP02753PU-N | DAB1 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P |
0.1 mg /
€300.00
|
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| OriGene Technologies GmbH | |||||||
| AP02753PU-S | DAB1 antibody |
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Rabbit | Aff - Purified | Hu, Ms, Rt | P |
50 µg /
€230.00
|
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| OriGene Technologies GmbH | |||||||
| AP05071PU-N | DAB1 (C-term) antibody |
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 |
Sheep | IgG | Purified | Ms | IP, WB |
0.25 mg /
€400.00
|
|
| OriGene Technologies GmbH | |||||||
| AP20741PU-N | DAB1 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
| AP20742PU-N | DAB1 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
| AP39027PU-N | DAB1 antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
50 µl /
€425.00
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|
| OriGene Technologies GmbH | |||||||
 +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP314664 | DAB1 |
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| Recombinant protein of human disabled homolog 1 (Drosophila) (DAB1) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402829 | DAB1 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR220941L2V | Lenti ORF particles, Dab1 (GFP-tagged) - Mouse disabled homolog 1 (Drosophila) (Dab1), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR220947L2V | Lenti ORF particles, Dab1 (GFP-tagged) - Mouse disabled homolog 1 (Drosophila) (Dab1), transcript variant 2, 200 uL, >10^7 TU/mL |
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|
200 µl /
€1,260.00
|
|
| OriGene Technologies, Inc. | ||
| MR220941L1V | Lenti ORF particles, Dab1 (Myc-DDK-tagged) - Mouse disabled homolog 1 (Drosophila) (Dab1), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
| MR220947L1V | Lenti ORF particles, Dab1 (Myc-DDK-tagged) - Mouse disabled homolog 1 (Drosophila) (Dab1), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,140.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR202584L2V | Lenti ORF particles, Dab1 (GFP-tagged ORF) - Rat disabled homolog 1 (Drosophila) (Dab1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,260.00
|
|
| OriGene Technologies, Inc. | ||
| RR202584L1V | Lenti ORF particles, Dab1 (Myc-DDK-tagged ORF) - Rat disabled homolog 1 (Drosophila) (Dab1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,130.00
|
|
| OriGene Technologies, Inc. | ||