DARS1 antibody
Principal name
DARS1 antibody
Alternative names for DARS1 antibody
DARS, PIG40, AspRS, Aspartate-tRNA ligase, Aspartyl-tRNA synthetase cytoplasmic, Cell proliferation-inducing gene 40 protein
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of DARS1 antibody
- Kit Component:
- - KN200391G1, DARS gRNA vector 1 in pCas-Guide vector
- KN200391G2, DARS gRNA vector 2 in pCas-Guide vector
- KN200391D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Guzzo C.M., et al. (2008) Biochem. Biophys. Res. Commun. 365:718-723Imami K., et al. (2001) Anal. Sci. 24:161-166
Immunoperoxidase of monoclonal antibody to DARS on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
Western blot analysis of DARS over-expressed 293 cell line, cotransfected with DARS Validated Chimera RNAi ( Cat # H00001615-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DARS monoclonal antibody (M01), clone 2F11 (Cat # H00001615-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
DARS monoclonal antibody (M01), clone 2F11 Western Blot analysis of DARS expression in HeLa ( Cat # L013V1 ).
Western Blot analysis of DARS expression in transfected 293T cell line by DARS monoclonal antibody (M01), clone 2F11.Lane 1: DARS transfected lysate(57.1 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged DARS is approximately 0.3ng/ml as a capture antibody.
Immunofluorescence of purified MaxPab antibody to DARS on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of DARS expression in transfected 293T cell line (H00001615-T01) by DARS MaxPab polyclonal antibody.Lane 1: DARS transfected lysate(55.11 KDa).Lane 2: Non-transfected lysate.
Human Brain, cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Ganglion cells: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of DARS over-expressed 293 cell line, cotransfected with DARS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DARS monoclonal antibody, clone 2F11.
DARS monoclonal antibody, clone 2F11 Western Blot analysis of DARS expression in HeLa.
Western Blot analysis of DARS expression in transfected 293T cell line by DARS monoclonal antibody, clone 2F11.
Detection limit for recombinant GST tagged DARS is approximately 0.3 ng/ml as a capture antibody.
Formalin fixed and paraffin embedded human liver tissue stained with DARS1 Antibody (N-term) Cat.-No AP51185PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of DARS1 Antibody (N-term) Cat.-No AP51185PU-N with HepG2 cell followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of DARS (arrow) using DARS1 Antibody (N-term) Cat.-No AP51185PU-N.
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DARS gene.
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DARS gene.
Western blot analysis of DARS1 Antibody (N-term) Cat.-No AP51185PU-N in Jurkat cell line lysates (35ug/lane). This demonstrates the DARS antibody detected the DARS protein (arrow).
Host: Rabbit; Target Name: DARS; Sample Tissue: Human Fetal Lung ; Antibody Dilution: 1.0ug/ml
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP51185PU-N | DARS1 (N-term) antibody |
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Rabbit | Ig | Purified | Hu | ICC/IF, P, WB |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300391 | DARS1 |
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| Recombinant protein of human aspartyl-tRNA synthetase (DARS) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR207016L2V | Lenti ORF particles, Dars (GFP-tagged ORF) - Rat aspartyl-tRNA synthetase (Dars), 200 uL, >10^7 TU/mL |
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 |
200 µl /
€1,190.00
|
|
| OriGene Technologies, Inc. | ||
| RR207016L1V | Lenti ORF particles, Dars (Myc-DDK-tagged ORF) - Rat aspartyl-tRNA synthetase (Dars), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,060.00
|
|
| OriGene Technologies, Inc. | ||
