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DCP1A antibody

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Principal name

DCP1A antibody

Alternative names for DCP1A antibody

SMIF, mRNA-decapping enzyme 1A, Transcription factor SMIF, Smad4-interacting transcriptional co-activator

SwissProt ID

Q91YD3 (Mouse), Q9NPI6 (Human)

Gene ID

55802 (DCP1A), 75901 (Dcp1a)

Ncbi ID

9606, NP_060873, NP_060873.4, NM_133761

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of DCP1A antibody

Signal transduction of TGF-beta superfamily members is regulated by Smad proteins. In particular, Smads influence specific gene transcription by relaying signals from the cell membrane to the nucleus. Smad4 plays an essential role in TGF-beta-induced transcriptional activation wherein phosphorylated receptor-associated Smads associate with Smad4. Furthermore, SMIF (Smad4-intereacting protein) and Smad4 complex with TGF-beta and BMP4. An increase in Smad4 concentration increases the translocation of this complex to the nucleus. SMIF and Smad4 interact directly through a EVH1/WH1 domain on SMIF and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of SMIF as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of SMIF eliminates TGF-β-induced nuclear translocation of SMIF (1). The human SMIF gene is ubiquitously expressed and encodes a protein with a relative molecular mass of 70 kDa.

General readings

Lehner,B. et al., (2004) Genome Res. 14 (7), 1315-1323

H00055802-M06 - DCP1A
Immunofluorescence of monoclonal antibody to DCP1A on HeLa cell. [antibody concentration 10 ug/ml]
H00055802-M06 - DCP1A
DCP1A monoclonal antibody (M06), clone 3G4 Western Blot analysis of DCP1A expression in IMR-32 ( Cat # L008V1 ).
H00055802-M06 - DCP1A
Detection limit for recombinant GST tagged DCP1A is approximately 0.1ng/ml as a capture antibody.
H00055802-A01 - DCP1A
DCP1A polyclonal antibody (A01), Lot # 060428JCS1. Western Blot analysis of DCP1A expression in Raw 264.7.
ARP39353_T100 - DCP1A
Human Jurkat; WB Suggested Anti-DCP1A Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. DCP1A is supported by BioGPS gene expression data to be expressed in Jurkat.; DCP1A antibody - N-terminal region (ARP39353_T100) in Human Jurkat cells using Western Blot
ARP34582_T100 - DCP1A
Human Jurkat; WB Suggested Anti-DCP1A Antibody Titration: 2.5ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. DCP1A is supported by BioGPS gene expression data to be expressed in Jurkat.; DCP1A antibody - N-terminal region (ARP34582_T100) in Human Jurkat cells using Western Blot
AP06755PU-N - DCP1A
Western blot (WB) analysis of SMIF antibody (Cat.-No.: AP06755PU-N) in extracts from Jurkat cells.
AP06755PU-N - DCP1A
Immunohistochemistry (IHC) analyzes of SMIF antibody (Cat.-No.: AP06755PU-N) on paraffin-embedded sections.
PAB6970 - DCP1A
DCP1A polyclonal antibody ( Cat # PAB6970 ) ( 2 µg/ml ) staining of A-431 cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
H00055802-M01 - DCP1A
Western Blot analysis of DCP1A expression in transfected 293T cell line by DCP1A monoclonal antibody (M01), clone 2D12.Lane 1: DCP1A transfected lysate(63.3 KDa).Lane 2: Non-transfected lysate.
H00055802-M01 - DCP1A
DCP1A monoclonal antibody (M01), clone 2D12. Western Blot analysis of DCP1A expression in IMR-32 ( Cat # L008V1 ).
H00055802-M02 - DCP1A
DCP1A monoclonal antibody (M02), clone 2F11. Western Blot analysis of DCP1A expression in IMR-32 ( Cat # L008V1 ).
H00055802-M07 - DCP1A
DCP1A monoclonal antibody (M07), clone 2G10. Western Blot analysis of DCP1A expression in IMR-32 ( Cat # L008V1 ).
H00055802-B01P - DCP1A
DCP1A MaxPab polyclonal antibody. Western Blot analysis of DCP1A expression in rat brain.
H00055802-B01P - DCP1A
Western Blot analysis of DCP1A expression in transfected 293T cell line (H00055802-T01) by DCP1A MaxPab polyclonal antibody.Lane 1: DCP1A transfected lysate(64.02 KDa).Lane 2: Non-transfected lysate.
H00055802-D01 - DCP1A
Immunoprecipitation of DCP1A transfected lysate using anti-DCP1A MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with DCP1A MaxPab mouse polyclonal antibody (B01) (H00055802-B01).
C11740-1 - DCP1A
Western blot analysis of extracts from Jurkat cells, using DCP1A Antibody. The lane on the right is treated with the synthesized peptide.
TP301330 - DCP1A
TA303086 - DCP1A
TA303086 (2µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA310147 - DCP1A
Western blot analysis of HeLa cell lysates with anti-Dcp1a antibody (1:500).
TA326292 - DCP1A
Western blot analysis of extracts from Jurkat cells, using DCP1A antibody.The lane on the right is treated with the synthesized peptide.
TA337231 - DCP1A
WB Suggested Anti-DCP1A Antibody Titration: 2.5 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysateDCP1A is supported by BioGPS gene expression data to be expressed in Jurkat
TA345251 - DCP1A
WB Suggested Anti-DCP1A Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate; DCP1A is supported by BioGPS gene expression data to be expressed in Jurkat
TA347656 - DCP1A
Western blot analysis on Jurkat cell lysate using DCP1A Antibody

8 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DCP1A antibody
  Goat Polyclonal Antibody against DCP1A  
TA303086 (2µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

DCP1A antibody
  Rabbit anti-Dcp1a polyclonal antibody  
Western blot analysis of HeLa cell lysates with anti-Dcp1a antibody (1:500). Rabbit Purified Hu, Ms WB
0.1 ml / €220.00
  OriGene Technologies, Inc.

DCP1A antibody
  Rabbit polyclonal DCP1A antibody  
Western blot analysis of extracts from Jurkat cells, using DCP1A antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

DCP1A antibody
  Rabbit Polyclonal Anti-DCP1A Antibody  
Western blot analysis on Jurkat cell lysate using DCP1A Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

DCP1A antibody
Western blot (WB) analysis of SMIF antibody (Cat.-No.: AP06755PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DCP1A
  Recombinant protein of human DCP1 decapping enzyme homolog A (S. cerevisiae) (DCP1A)  
DCP1A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Dcp1a (GFP-tagged) - Mouse DCP1 decapping enzyme homolog A (S. cerevisiae) (Dcp1a), 200 uL, >10^7 TU/mL
Lenti ORF particles, Dcp1a (GFP-tagged) - Mouse DCP1 decapping enzyme homolog A (S. cerevisiae) (Dcp1a), 200 uL, >10^7 TU/mL  
200 µl / €1,330.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Dcp1a (Myc-DDK-tagged) - Mouse DCP1 decapping enzyme homolog A (S. cerevisiae) (Dcp1a), 200 uL, >10^7 TU/mL
Lenti ORF particles, Dcp1a (Myc-DDK-tagged) - Mouse DCP1 decapping enzyme homolog A (S. cerevisiae) (Dcp1a), 200 uL, >10^7 TU/mL  
200 µl / €1,190.00
  OriGene Technologies, Inc.
 

8 Item(s)

per page

Available Filters

Product Type
  1. Mouse Lenti ORF Particle (2)
  2. Primary Antibody (5)
  3. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (5)
Target
  1. DCP1A (6)
Reactivity
  1. Human (Hu) (5)
  2. Mouse (Ms) (5)
  3. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Paraffin Sections (P) (1)
Presentation
  1. Purified (3)
  2. Aff - Purified (2)
Host
  1. Rabbit (4)
  2. Goat (1)
Images
  1. 1 image (6)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (8)
Service
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