DCUN1D1 antibody
Principal name
DCUN1D1 antibody
Alternative names for DCUN1D1 antibody
DCN1-like protein 1, DCUN1L1, RP42, SCCRO, SCRO, Tes3, Defective in cullin neddylation protein 1-like protein 1, Squamous cell carcinoma-related oncogene
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)
Background of DCUN1D1 antibody
- Kit Component:
- - KN304348G1, Dcun1d1 gRNA vector 1 in pCas-Guide vector
- KN304348G2, Dcun1d1 gRNA vector 2 in pCas-Guide vector
- KN304348D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Estilo C.L., et al. (2003). Clin. Cancer Res, 9: 2300-2306.
Lum L., et al. (2004). Science., 304: 1755-1759.
Immunofluorescence of monoclonal antibody to DCUN1D1 on HeLa cell. [antibody concentration 10 ug/ml]
DCUN1D1 monoclonal antibody (M01), clone 3D7. Western Blot analysis of DCUN1D1 expression in NIH/3T3(Cat # L018V1 ).
DCUN1D1 monoclonal antibody (M01), clone 3D7. Western Blot analysis of DCUN1D1 expression in Raw 264.7(Cat # L024V1 ).
DCUN1D1 monoclonal antibody (M01), clone 3D7 Western Blot analysis of DCUN1D1 expression in HepG2 ( Cat # L019V1 ).
DCUN1D1 monoclonal antibody (M01), clone 3D7. Western Blot analysis of DCUN1D1 expression in PC-12(Cat # L012V1 ).
DCUN1D1 polyclonal antibody (A01), Lot # 060524JCS1 Western Blot analysis of DCUN1D1 expression in Hela NE ( Cat # L013V3 ).
DCUN1D1 monoclonal antibody (M02), clone 4B5. Western Blot analysis of DCUN1D1 expression in NIH/3T3(Cat # L018V1 ).
DCUN1D1 monoclonal antibody (M02), clone 4B5. Western Blot analysis of DCUN1D1 expression in Raw 264.7(Cat # L024V1 ).
DCUN1D1 monoclonal antibody (M02), clone 4B5. Western Blot analysis of DCUN1D1 expression in HepG2 ( Cat # L019V1 ).
DCUN1D1 monoclonal antibody (M02), clone 4B5. Western Blot analysis of DCUN1D1 expression in PC-12(Cat # L012V1 ).
Western Blot analysis of DCUN1D1 expression in transfected 293T cell line (H00054165-T01) by DCUN1D1 MaxPab polyclonal antibody.Lane 1: DCUN1D1 transfected lysate(28.49 KDa).Lane 2: Non-transfected lysate.
Human kidney; DCUN1D1 antibody - N-terminal region (ARP42570_P050) in Human kidney cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-DCUN1D1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate. DCUN1D1 is supported by BioGPS gene expression data to be expressed in Jurkat.; DCUN1D1 antibody - N-terminal region (ARP42570_P050) in Human Jurkat cells using Western Blot
DCUN1D1 MaxPab rabbit polyclonal antibody. Western Blot analysis of DCUN1D1 expression in mouse liver.
Western Blot analysis of DCUN1D1 expression in transfected 293T cell line (H00054165-T02) by DCUN1D1 MaxPab polyclonal antibody.Lane 1: DCUN1D1 transfected lysate(30.10 KDa).Lane 2: Non-transfected lysate.
DCUN1D1 MaxPab rabbit polyclonal antibody. Western Blot analysis of DCUN1D1 expression in mouse liver.
Immunoprecipitation of DCUN1D1 transfected lysate using anti-DCUN1D1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with DCUN1D1 MaxPab mouse polyclonal antibody (B01) (H00054165-B01).
Human PANC1; WB Suggested Anti-DCUN1D1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: PANC1 cell lysate. DCUN1D1 is supported by BioGPS gene expression data to be expressed in PANC1.; DCUN1D1 antibody - middle region (ARP42571_P050) in Human PANC1 cells using Western Blot
Recombinant human DCUN1D1, 1-259aa, His-tagged
(0.5ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-DCUN1D1 antibody (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
WB Suggested Anti-DCUN1D1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysateDCUN1D1 is supported by BioGPS gene expression data to be expressed in Jurkat
Human kidney
WB Suggested Anti-DCUN1D1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: PANC1 cell lysateDCUN1D1 is supported by BioGPS gene expression data to be expressed in PANC1
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA311491 | DCUN1D1 antibody |
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| Goat Anti-DCUN1D1 Antibody | |||||||
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Goat | Aff - Purified | Bov, Hu, Ms | E, P, WB |
0.1 mg /
€360.00
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303427 | DCUN1D1 |
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| Recombinant protein of human DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) (DCUN1D1) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP720243 | DCUN1D1 |
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| Recombinant protein of human DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) (DCUN1D1) | |||||
 |
Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
€299.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203355L2V | Lenti ORF particles, Dcun1d1 (GFP-tagged) - Mouse DCUN1D1 DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) (cDNA clone MGC:28191, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR203355L1V | Lenti ORF particles, Dcun1d1 (Myc-DDK-tagged) - Mouse DCUN1D1 DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) (cDNA clone MGC:28191, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||